热毒宁注射液对细菌内毒素性脂多糖致热大鼠的解热作用研究(3)
[11] Sugiyama S， Kugiyama K， Aikawa M， et， al. Hypochlorous acid， a macrophage product， induces endothelial apoptosis and tissue factor expression involvement of myeloperoxidase mediated oxidant in plaque erosin and thrombogenesis[J]. Arterioscler thromb Vasc Bid， 2004， 24(7)：1309.
[12] 王兰兰，王乐，曹宇.在LPS致大鼠发热过程中下丘脑TRPV4对体温及cAMP含量、[Ca2+]浓度的影响[J]. 中国药理学通报， 2008， 24(9)：1174.
Study on antipyretic effect of Reduning injection on
lipopolysaccharide-induced fever rats
TANG Lu-ping 1， HE Rong-rong 1， LI Yi-fang 1， LI Hai-bo 2， YAO Xin-sheng 1， Hiroshi Kurihara1*， XIAO Wei 2*
(1. Pharmacy College， Jinan University， Guangzhou 510632， China；
2. State Key Laboratory of New-tech for Chinese Medicine Pharmaceutical Process， Lianyungang 222001， China)
[Abstract] To observe the antipyretic effect of Reduning injection (RDN) on lipopolysaccharide (LPS)-induced fever rats and its impact on centric fever medium. Rats were randomly divided into the blank control group， the model group， the Metamizole group， and high and low-dose RDN groups. Except for the blank control group， all of the rats were injected intraperitoneally with LPS (80 μg·kg-1) to observe their body temperature changes. The double-antibody sandwich ELSIA method was adopted to determine cAMP content in hypothalamus and MPO in lung tissues of fever peak rats. The high-dose RDN group can obviously reduce the temperature rise in fever rats， and cAMP and MPO content in hypothalamus. RDN showed significant antipyretic effect， which may be related with the reduction of cAMP content in hypothalamus and MPO in lung tissues.
[Key words] Reduning injection； antipyretic； cAMP； MPO
doi：10.4268/cjcmm20131432, http://www.100md.com(唐陆平 何蓉蓉 李怡芳 李海波 姚新生 栗原博 萧伟)
[12] 王兰兰，王乐，曹宇.在LPS致大鼠发热过程中下丘脑TRPV4对体温及cAMP含量、[Ca2+]浓度的影响[J]. 中国药理学通报， 2008， 24(9)：1174.
Study on antipyretic effect of Reduning injection on
lipopolysaccharide-induced fever rats
TANG Lu-ping 1， HE Rong-rong 1， LI Yi-fang 1， LI Hai-bo 2， YAO Xin-sheng 1， Hiroshi Kurihara1*， XIAO Wei 2*
(1. Pharmacy College， Jinan University， Guangzhou 510632， China；
2. State Key Laboratory of New-tech for Chinese Medicine Pharmaceutical Process， Lianyungang 222001， China)
[Abstract] To observe the antipyretic effect of Reduning injection (RDN) on lipopolysaccharide (LPS)-induced fever rats and its impact on centric fever medium. Rats were randomly divided into the blank control group， the model group， the Metamizole group， and high and low-dose RDN groups. Except for the blank control group， all of the rats were injected intraperitoneally with LPS (80 μg·kg-1) to observe their body temperature changes. The double-antibody sandwich ELSIA method was adopted to determine cAMP content in hypothalamus and MPO in lung tissues of fever peak rats. The high-dose RDN group can obviously reduce the temperature rise in fever rats， and cAMP and MPO content in hypothalamus. RDN showed significant antipyretic effect， which may be related with the reduction of cAMP content in hypothalamus and MPO in lung tissues.
[Key words] Reduning injection； antipyretic； cAMP； MPO
doi：10.4268/cjcmm20131432, http://www.100md.com(唐陆平 何蓉蓉 李怡芳 李海波 姚新生 栗原博 萧伟)