n分析> 苦碟子药材及其注射液中化学成分的HPLC—ESI—MSn分析(5)
LIU Ying1，2， LU Jian-qiu1， ZHAGN Jia-yuWANG Fang2， WANG Qing2， QIAO Yan-jiang(1. Center of Scientific Experiment， Beijing University of Chinese Medicine， Beijing 100029， China；
2. School of Chinese Pharmacy， Beijing University of Chinese Medicine， Beijing 100102， China)
[Abstract] A rapid analytical method was developed for the identification of components in Ixeris sonchifolia and its injection by HPLC-ESI-MSn. The characterization of various components was analyzed according to the retention time of reference standard and mass spectrometry information. A profile of I. sonchifolia and its injection constituents was described and a total of three nucleosides， sixteen phenolic acids， seven flavonoids and twelve sesquiterpene lactones were identified or tentatively characterised. Twelve compounds were accurately identified with reference standards. Eleven of them， guanosine， 1-caffeoylquinic acid， 3-caffeoylquinic acid， 4-caffeoylquinic acid， caffeic anhydride， 3，4-dicaffeoylquinic acid， 3，5-dicaffeoylquinic acid， 3-p-coumaroylquinic acid， 3-feruloylquinic acid， 5-p-coumaroylquinic acid and 4-feruloylquinic acid were reported for the first time in I. sonchifolia and its preparations. The structures of five isomers were deduced by theirs mass information and the fragmentation pattern of known compounds. The developed method was useful for the quality control and evaluation of this herb and its preparations. The HPLC-ESI-MSn could be a promising tool for the rapid analysis of components from herbal medicines.
[Key words] Ixeris sonchifolia ； HPLC-ESI-MSn； fragmentation pattern； nucleosides； phenolic acids； flavonoids； sesquiterpene lactones
doi：10.4268/cjcmm20131627
[责任编辑 孔晶晶], http://www.100md.com(刘颖 卢建秋 张加余 王放 王青 乔延江)
2. School of Chinese Pharmacy， Beijing University of Chinese Medicine， Beijing 100102， China)
[Abstract] A rapid analytical method was developed for the identification of components in Ixeris sonchifolia and its injection by HPLC-ESI-MSn. The characterization of various components was analyzed according to the retention time of reference standard and mass spectrometry information. A profile of I. sonchifolia and its injection constituents was described and a total of three nucleosides， sixteen phenolic acids， seven flavonoids and twelve sesquiterpene lactones were identified or tentatively characterised. Twelve compounds were accurately identified with reference standards. Eleven of them， guanosine， 1-caffeoylquinic acid， 3-caffeoylquinic acid， 4-caffeoylquinic acid， caffeic anhydride， 3，4-dicaffeoylquinic acid， 3，5-dicaffeoylquinic acid， 3-p-coumaroylquinic acid， 3-feruloylquinic acid， 5-p-coumaroylquinic acid and 4-feruloylquinic acid were reported for the first time in I. sonchifolia and its preparations. The structures of five isomers were deduced by theirs mass information and the fragmentation pattern of known compounds. The developed method was useful for the quality control and evaluation of this herb and its preparations. The HPLC-ESI-MSn could be a promising tool for the rapid analysis of components from herbal medicines.
[Key words] Ixeris sonchifolia ； HPLC-ESI-MSn； fragmentation pattern； nucleosides； phenolic acids； flavonoids； sesquiterpene lactones
doi：10.4268/cjcmm20131627
[责任编辑 孔晶晶], http://www.100md.com(刘颖 卢建秋 张加余 王放 王青 乔延江)