2+在水杨酸诱导丹参幼苗迷迭香酸生物合成过程中的作用> 胞内、外Ca2+在水杨酸诱导丹参幼苗(7)
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Regulation effects of intracellular and extracellular Ca2+ on biosynthesis of
rosmarinic acid induced by salicylic acid in young seedlings of Salvia miltiorrhiza
CAO Rong-rong， DANG Xiao-lin， XING Bing-yu， ZHANG Jing-yi， DONG Juan-e*
(College of Life Sciences， Northwest Agricultural and Forestry University， Yangling 712100， China)
[Abstract] Objective： To investigate the effect of intracellular and extracellular Ca2+ on the biosynthesis of rosmarinic acid (RA) induced by salicylic acid in young seedlings of Salvia miltiorrhiza. Method： Young seedlings of S. miltiorrhiza were used to select an optimal concentration of salicylic acid(SA)， and then use the optimal concentration of SA to investigate the effects of extracellular Ca2+ channel inhibitors Verapamil，LaCl3， intracelluar calmodulin antagonist TFP and intracelluar Ca2+ channel inhibitors LiCl on the biosynthesis of RA and related enzymes. Result： SA increased the accumulation of RA and the activities of PAL and TAT， especially the SA of 2 mmol·L-1 after 24 h. SA improved the accumulation of RA to (40.51±2.16) mg·g1， which was 1.97 times than that of control， and the activities of PAL， TAT were 1.42 times and 1.29 times than those of the control. However， Vp，LaCl3， TFP， LiCl inhibited the effects of SA evidently. Conclusion： Ca2+ plays a key role in the regulation of the induction process.
[Key words] Salvia miltiorrhiza； salicylic acid； PAL and TAT； rosmarinic acid； Ca2+ channel blockers； calmodulin antagonist
doi：10.4268/cjcmm20132004, 百拇医药(曹蓉蓉 党小琳 行冰玉 张婧一 董娟娥)
Regulation effects of intracellular and extracellular Ca2+ on biosynthesis of
rosmarinic acid induced by salicylic acid in young seedlings of Salvia miltiorrhiza
CAO Rong-rong， DANG Xiao-lin， XING Bing-yu， ZHANG Jing-yi， DONG Juan-e*
(College of Life Sciences， Northwest Agricultural and Forestry University， Yangling 712100， China)
[Abstract] Objective： To investigate the effect of intracellular and extracellular Ca2+ on the biosynthesis of rosmarinic acid (RA) induced by salicylic acid in young seedlings of Salvia miltiorrhiza. Method： Young seedlings of S. miltiorrhiza were used to select an optimal concentration of salicylic acid(SA)， and then use the optimal concentration of SA to investigate the effects of extracellular Ca2+ channel inhibitors Verapamil，LaCl3， intracelluar calmodulin antagonist TFP and intracelluar Ca2+ channel inhibitors LiCl on the biosynthesis of RA and related enzymes. Result： SA increased the accumulation of RA and the activities of PAL and TAT， especially the SA of 2 mmol·L-1 after 24 h. SA improved the accumulation of RA to (40.51±2.16) mg·g1， which was 1.97 times than that of control， and the activities of PAL， TAT were 1.42 times and 1.29 times than those of the control. However， Vp，LaCl3， TFP， LiCl inhibited the effects of SA evidently. Conclusion： Ca2+ plays a key role in the regulation of the induction process.
[Key words] Salvia miltiorrhiza； salicylic acid； PAL and TAT； rosmarinic acid； Ca2+ channel blockers； calmodulin antagonist
doi：10.4268/cjcmm20132004, 百拇医药(曹蓉蓉 党小琳 行冰玉 张婧一 董娟娥)