凉血化瘀方拮抗内质网应激引起的L02肝细胞凋亡作用及机制研究(4)
(Nanjing University of Chinese Medicine， Nanjing 210023， China)
[Abstract] Endoplasmic reticulum stress (ERS) is a new pathway inducing cell apoptosis that has been discovered in recent years. This study focused on the protective effect of Liangxue Huayu recipe (LHR) on tumor necrosis factor-α (TNF-α) and D-GalN-induced hepatocyte apoptosis. It found that TNF-α and D-GalN could obviously inhibit hepatocyte proliferation， induce cell apoptosis， and significantly increase free calcium ions in cytoplasms， as well as protein expressions of ERS apoptosis-related signal molecules phosphorylated PERK， phosphorylated elF2α， cleaved Caspase-12， GRP78 and CHOP. After the administration of LHR of different concentrations， compared with the TNF-α/GalN injury group， LHR could significantly alleviated L02 hepatocyte proliferation， decreased cell apoptosis， inhibited growth of intracytoplasmic free calcium content， and gradually reduced the protein expressions of phosphorylated PERK， phosphorylated elF2α， cleaved Caspase-12， GRP78 and CHOP. These findings indicated that LHR has the inhibitory effect on TNF-α and D-GalN-induced hepatocyte apoptosis. Its mechanism may be related to down-regulation of ERS apoptosis-related signal molecules phosphorylated PERK， phosphorylated elF2α， cleaved Caspase-12， GRP78 and CHOP that maintain calcium homeostasis in endoplasmic reticulum.
[Key words] Liangxue Huayu recipe (LHR)； hepatocyte apoptosis； endoplasmic reticulum stress (ERS)
doi：10.4268/cjcmm20132027, 百拇医药(姜泽群 姚志华 邓正亭 蒋鑫超 颜晓静 陈卫平)
[Abstract] Endoplasmic reticulum stress (ERS) is a new pathway inducing cell apoptosis that has been discovered in recent years. This study focused on the protective effect of Liangxue Huayu recipe (LHR) on tumor necrosis factor-α (TNF-α) and D-GalN-induced hepatocyte apoptosis. It found that TNF-α and D-GalN could obviously inhibit hepatocyte proliferation， induce cell apoptosis， and significantly increase free calcium ions in cytoplasms， as well as protein expressions of ERS apoptosis-related signal molecules phosphorylated PERK， phosphorylated elF2α， cleaved Caspase-12， GRP78 and CHOP. After the administration of LHR of different concentrations， compared with the TNF-α/GalN injury group， LHR could significantly alleviated L02 hepatocyte proliferation， decreased cell apoptosis， inhibited growth of intracytoplasmic free calcium content， and gradually reduced the protein expressions of phosphorylated PERK， phosphorylated elF2α， cleaved Caspase-12， GRP78 and CHOP. These findings indicated that LHR has the inhibitory effect on TNF-α and D-GalN-induced hepatocyte apoptosis. Its mechanism may be related to down-regulation of ERS apoptosis-related signal molecules phosphorylated PERK， phosphorylated elF2α， cleaved Caspase-12， GRP78 and CHOP that maintain calcium homeostasis in endoplasmic reticulum.
[Key words] Liangxue Huayu recipe (LHR)； hepatocyte apoptosis； endoplasmic reticulum stress (ERS)
doi：10.4268/cjcmm20132027, 百拇医药(姜泽群 姚志华 邓正亭 蒋鑫超 颜晓静 陈卫平)