滇重楼内生真菌分离与多样性研究(5)
[Abstract] The paper is aimed at studying the diversity of endophytic fungi community from Paris polyphylla var.yunnanensis， and to provide a scientific basis for the utilization value of the endophytic fungi as bioactive material resources.In the present study， endophytic fungi were isolated from roots， rhizomes and leaves of wild P.polyphylla var.yunnanensis collected from Baoshan， Heqing county and Songming city of Yunnan province， and identified and classified by morphological methods together with its ITS sequence analysis.Seven and forty-nine strains of endophytic fungi were isolated from P.polyphylla var.yunnanensis.They were identified belonging to 41 genus.In these 41 genus， 3 genus exist in root only， 12 genus only exist in rhizome and 8 genus only exist in leaf.There was difference in endophytic fungi isolated from different sample sites.Endophytic fungi diversity from rhizomes of Heqing site was the highest.Endophytic fungi similarity coefficient was low among different sites and tissues.Based on these results， it is reasonable to propose that endophytic fungi of P.polyphylla var.yunnanensis from different tissue and different sample sites has a certain difference which is possibly relate to their different habitats， different structure and composition of each tissue.
[Key words] Paris polyphylla var.yunnanensis； endophytic fungi； isolation； identification； diversity
doi：10.4268/cjcmm20132209
[责任编辑 吕冬梅], http://www.100md.com(王茜 申仕康 张爱丽 吴春妍 吴富勤 张新军 王跃华)
[Key words] Paris polyphylla var.yunnanensis； endophytic fungi； isolation； identification； diversity
doi：10.4268/cjcmm20132209
[责任编辑 吕冬梅], http://www.100md.com(王茜 申仕康 张爱丽 吴春妍 吴富勤 张新军 王跃华)