诱导培养条件显著改变甘草愈伤组织次生代谢产物的多样性(4)
diversity of callus cell lines of Glycyrrhiza sp.
LIU Feng-cai1， LV Jian-ming2， WU Xiu-zhen1，2， ZHANG Wei1，2*
(1.Department of Biochemical Engineering， School of Chemical Engineering， Tianjin University， Tianjin 300072， China；
2.Tianjin Acelbio Biotechnology Co.， Ltd.， Tianjin International Joint Academy of Biotechnology and Medicine，Tianjin 300457， China)
[Abstract] The purpose of this study was to evaluate the impact of callus induction and culture conditions on secondary metabolic diversity of the callus cell lines of traditional Chinese medicinal plant Glycyrrhiza sp. (Glycyrrhiza) by combined chemical analysis and HPLC fingerprint. These callus induction conditions included two Glycyrrhiza species， two types of explants， light and dark conditions， and two combinations of hormones. The evaluation was firstly based on the contents of total flavonoids in the callus by chemical analysis and one way ANOVA. The content of total flavonoids in callus was significantly (P<0.05) influenced by Glycyrrhiza species， light condition， and the combination of hormones. The callus was further evaluated using diversity factor based on the comparison of HPLC fingerprints of these callus cell lines. Diversity factor varies significantly for calli induced under different conditions， with the highest being at 0.45 under light condition and combination of hormones. These results provide important knowledge for the selection of suitable callus cell lines for the production of pharmacologically important secondary metabolites or bioactive fractions by in vitro culture of Glycyrrhiza sp.
[Key words] Glycyrrhiza； callus； secondary metabolite； fingerprint； HPLC； diversity factor
doi：10.4268/cjcmm20132313, 百拇医药(刘凤彩 吕建明 吴秀祯 张卫)
LIU Feng-cai1， LV Jian-ming2， WU Xiu-zhen1，2， ZHANG Wei1，2*
(1.Department of Biochemical Engineering， School of Chemical Engineering， Tianjin University， Tianjin 300072， China；
2.Tianjin Acelbio Biotechnology Co.， Ltd.， Tianjin International Joint Academy of Biotechnology and Medicine，Tianjin 300457， China)
[Abstract] The purpose of this study was to evaluate the impact of callus induction and culture conditions on secondary metabolic diversity of the callus cell lines of traditional Chinese medicinal plant Glycyrrhiza sp. (Glycyrrhiza) by combined chemical analysis and HPLC fingerprint. These callus induction conditions included two Glycyrrhiza species， two types of explants， light and dark conditions， and two combinations of hormones. The evaluation was firstly based on the contents of total flavonoids in the callus by chemical analysis and one way ANOVA. The content of total flavonoids in callus was significantly (P<0.05) influenced by Glycyrrhiza species， light condition， and the combination of hormones. The callus was further evaluated using diversity factor based on the comparison of HPLC fingerprints of these callus cell lines. Diversity factor varies significantly for calli induced under different conditions， with the highest being at 0.45 under light condition and combination of hormones. These results provide important knowledge for the selection of suitable callus cell lines for the production of pharmacologically important secondary metabolites or bioactive fractions by in vitro culture of Glycyrrhiza sp.
[Key words] Glycyrrhiza； callus； secondary metabolite； fingerprint； HPLC； diversity factor
doi：10.4268/cjcmm20132313, 百拇医药(刘凤彩 吕建明 吴秀祯 张卫)