药用真菌猪苓菌核渗出液理化性质的研究(4)
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Physicochemical properties of medicinal fungus
Polyporus umbellatus sclerotial exudate
XING Yong-mei， LI Hong-lian， GUO Shun-xing
(1. Institute of Medicinal Plant Development， Chinese Academy of Medical Sciences &
Peking Union Medical College， Beijing 100193， China；
2. Agriculture Committee of Guxian Country， Guxian 042400， China)
[Abstract] This study was conducted to investigate the physicochemical properties of Polyporus umbellatus sclerotial exudate. Morphological characteristics of the sclerotia and its exudate were observed during different stages of sclerotial formation. The pH of the exudate was detected at different time during cultivation. A phenol-sulfuric acid method was employed to determine the polysaccharide content of P. umbellatus sclerotial exudate during cultivating time. Additionally， the protein content was measured by means of BCA protein assay. Furthermore， CAT content was detected using ultraviolet absorption method. That the protein content of the exudate and CAT specific activity rose gradually during the passage of the cultivating time indicated a high level of oxidative stress during P. umbellatus sclerotial exudate formation. The results showed that the pH of the exudate increased gradually and then dropped down during sclerotial formation. That the pH of the exudate maintained the acidity state during the cultivation indirectly indicated that acidic environment would help sclerotial formation. The exudate produced gradually and was absorbed by the sclerotia itself.
[Key words] Polyporus umbellatus； sclerotia； exudate； polysaccharide； CAT
doi：10.4268/cjcmm20140108
[责任编辑 吕冬梅], http://www.100md.com(邢咏梅 李红莲 郭顺星)
Physicochemical properties of medicinal fungus
Polyporus umbellatus sclerotial exudate
XING Yong-mei， LI Hong-lian， GUO Shun-xing
(1. Institute of Medicinal Plant Development， Chinese Academy of Medical Sciences &
Peking Union Medical College， Beijing 100193， China；
2. Agriculture Committee of Guxian Country， Guxian 042400， China)
[Abstract] This study was conducted to investigate the physicochemical properties of Polyporus umbellatus sclerotial exudate. Morphological characteristics of the sclerotia and its exudate were observed during different stages of sclerotial formation. The pH of the exudate was detected at different time during cultivation. A phenol-sulfuric acid method was employed to determine the polysaccharide content of P. umbellatus sclerotial exudate during cultivating time. Additionally， the protein content was measured by means of BCA protein assay. Furthermore， CAT content was detected using ultraviolet absorption method. That the protein content of the exudate and CAT specific activity rose gradually during the passage of the cultivating time indicated a high level of oxidative stress during P. umbellatus sclerotial exudate formation. The results showed that the pH of the exudate increased gradually and then dropped down during sclerotial formation. That the pH of the exudate maintained the acidity state during the cultivation indirectly indicated that acidic environment would help sclerotial formation. The exudate produced gradually and was absorbed by the sclerotia itself.
[Key words] Polyporus umbellatus； sclerotia； exudate； polysaccharide； CAT
doi：10.4268/cjcmm20140108
[责任编辑 吕冬梅], http://www.100md.com(邢咏梅 李红莲 郭顺星)