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http://www.100md.com 2014年1月15日 《中国中药杂志》 2014年第2期
     [21]Aldridge D C, Galt S, Giles D, et al. Metabolites of Lasiodiplodia theobromae [J]. J Chem Soc, 1971(C):1623.

    Study of production of sesquiterpenes of Aquilaria senensis

    stimulated by Lasiodiplodia theobromae

    HAN Xiao-min1, LIANG liang2, ZHANG Zheng3,4*, LI Xiu-jin1*, YANG Yun4, MENG Hui4, GAO Zhi-hui3, XU Yan-hong3

    (1.School of Environmental and Chemical Engineering, Yan Shan University, Qinhuangdao 066004, China;

    2.Shandong Traditional Chinese Medicine University, College of Pharmacy, Ji′nan 250012, China;

    3.Instituent of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking

    Union Medical College, Beijing 100193, China;

    4.Hainan Branch of Institute of Medicinal Plant Development, Chinese Academy of

    Medical Sciences & Peking Union Medical College,Hainan Provincial Key Laboratory of Resourse Conservation and Development of Southern Medicine, Wanning 571533, China)

    [Abstract]To investigate the mechanism of agarwood formation in Aquilaria sinensis induced by Lasiodiplodia theobromae, the fermentation liquor of L. theobromae was analyzed qualitatively and quantitatively by gas chromatography-mass spectrometry (GC-MS). JAs were detected in the fermentation liquor. The effect of the fermentation liquor on the abundance of sesquiterpenes in the callus of A. sinensis was analyzed by solid phase microextraction-gas chromatography-mass spectrometry (SPME-GC-MS). And the fermentation liquor stimulated α-guaiene, α-humulene and δ-guaiene biosynthesis in calli. It was inferred that L. theobromae produced JAs, which resulted in a significant increase of sesquiterpenes in A. sinensis.

    [Key words]Lasiodiplodia theobromae; jasmonates; Aquilaria senensis; sesquiterpenes

    doi:10.4268/cjcmm20140207

    [责任编辑吕冬梅], http://www.100md.com(韩晓敏 梁良 张争 李秀锦 杨云 孟慧 高志晖 徐艳红)
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