银杏外种皮多糖的单糖组成分析(4)
[13]Honda S，Akao E，Suzuki S，et al.High-performance liquid chroma-tography of reducing carbohydrates as strongly ultraviolet-absorbing and electrochemically sensitive 1-phenyl-3-methyl-5-pyrazolonederivatives[J].Anal Biochem，1989，180(2)：351.
[14]吕永磊，卜海博，杨蕾，等. 乌头母根子根须根多糖的比较[J].中国中药杂志，2011，36(9)：1154.
[15]Dai J，Wu Y， Chen S W，et al.Sugar compositional determination of polysaccharides from Dunaliella salina by modified RP-HPLC method of precolumn derivatization with 1-phenyl-3-methyl-5-pyrazolone[J].Carbohydr Polym，2010，82：629.
[16]宋根萍，许爱华，陈华圣，等.银杏外种皮多糖的成分分析[J].中药材，1997，20(9)：461.
Analysis of monosaccharide compositions in polysaccharides
from exopleura of Ginkgo biloba
MAO Lei-lei， CHEN Ying， HU Bi-yuan， XU Ai-hua*
(Medical College of Yangzhou University， Yangzhou 225001， China)
[Abstract]A high-performance liquid chromatography method of pre-column derivatization with 1-phenyl-3-methyl-5-pyrazolone (PMP) has been established for determination of 6 kinds of monosaccharides simultaneously. A special Agilent HC-C18 column (4.6 mm×250 mm，5 μm)， optimized for the separation of PMP derivatives， was used at ambient temperature of 40 ℃. The PMP derivatives elution was performed with a mixture of 0.1 mol·L-1 phosphate buffer (pH 6.8) and acetonitrile in a ratio of 84∶16 at a flow rate of 1 mL·min-1， and UV absorbance of the effluent was monitored at 245 nm. The results showed that the polysaccharides from exopleura of Ginkgo biloba were acidic heteropolysaccharides mainly containing mannose，rhamnose， D-galacturonic acid，glucose， galactose， arabinose， with the molar ratio of 0.032∶0.14∶0.296∶0.403∶0.106∶0.046.
[Key words]Ginkgo biloba； polysaccharides； monosaccharide； PMP； HPLC
doi：10.4268/cjcmm20140220
[责任编辑孔晶晶], 百拇医药(茅蕾蕾 陈颖 胡碧原 许爱华)
[14]吕永磊，卜海博，杨蕾，等. 乌头母根子根须根多糖的比较[J].中国中药杂志，2011，36(9)：1154.
[15]Dai J，Wu Y， Chen S W，et al.Sugar compositional determination of polysaccharides from Dunaliella salina by modified RP-HPLC method of precolumn derivatization with 1-phenyl-3-methyl-5-pyrazolone[J].Carbohydr Polym，2010，82：629.
[16]宋根萍，许爱华，陈华圣，等.银杏外种皮多糖的成分分析[J].中药材，1997，20(9)：461.
Analysis of monosaccharide compositions in polysaccharides
from exopleura of Ginkgo biloba
MAO Lei-lei， CHEN Ying， HU Bi-yuan， XU Ai-hua*
(Medical College of Yangzhou University， Yangzhou 225001， China)
[Abstract]A high-performance liquid chromatography method of pre-column derivatization with 1-phenyl-3-methyl-5-pyrazolone (PMP) has been established for determination of 6 kinds of monosaccharides simultaneously. A special Agilent HC-C18 column (4.6 mm×250 mm，5 μm)， optimized for the separation of PMP derivatives， was used at ambient temperature of 40 ℃. The PMP derivatives elution was performed with a mixture of 0.1 mol·L-1 phosphate buffer (pH 6.8) and acetonitrile in a ratio of 84∶16 at a flow rate of 1 mL·min-1， and UV absorbance of the effluent was monitored at 245 nm. The results showed that the polysaccharides from exopleura of Ginkgo biloba were acidic heteropolysaccharides mainly containing mannose，rhamnose， D-galacturonic acid，glucose， galactose， arabinose， with the molar ratio of 0.032∶0.14∶0.296∶0.403∶0.106∶0.046.
[Key words]Ginkgo biloba； polysaccharides； monosaccharide； PMP； HPLC
doi：10.4268/cjcmm20140220
[责任编辑孔晶晶], 百拇医药(茅蕾蕾 陈颖 胡碧原 许爱华)