LC—Q—TOF—MS及LC—IT—MS分析酸枣仁汤的化学成分(6)
Identification of constituents in Suanzaoren tang by
LC-Q-TOF-MS and LC-IT-MS
ZHU Yun-xiang， CHEN Lu-lin， GONG Jing-ru， WANG Shu-fang(Pharmaceutical Informatics Institute， Zhejiang University， Hangzhou 310058， China)
[Abstract] LC-Q-TOF-MS and LC-IT-MS in positive and negative ion mode were applied to simultaneously characterize the constituents in Suanzaoren tang. Analysis was performed on an Agilent Zorbax SB-C18， Rapid Resolution HT column(4.6 mm×50 mm，1.8 μm) with gradient elution of acetonitrile(A)-aqueous solution containing 0.05% formic acid(B) at a flow rate of 0.6 mL·min-1 and the column temperature was 30 ℃. By comparing MS fragmentation， accurate molecular weight， literature date and standard compounds information， a total of 48 compounds were successfully identified or speculated. The origins of these compounds were assigned to the corresponding Chinese medicine. Thirty-one compounds were reported in Suanzaoren tang for the first time. LC-Q-TOF-MS combined with LC-IT-MS is a simple and rapid tool for the identification of constituents of Suanzaoren tang， and the results could provide evidence for the research on quality combined and effective constituents of Suanzaoren tang.
[Key words] LC-Q-TOF-MS； LC-IT-MS； Suanzaoren tang； chemical costituents
doi：10.4268/cjcmm20140319
[责任编辑 孔晶晶], http://www.100md.com(朱云祥 陈璐琳 龚婧如 王书芳)
LC-Q-TOF-MS and LC-IT-MS
ZHU Yun-xiang， CHEN Lu-lin， GONG Jing-ru， WANG Shu-fang(Pharmaceutical Informatics Institute， Zhejiang University， Hangzhou 310058， China)
[Abstract] LC-Q-TOF-MS and LC-IT-MS in positive and negative ion mode were applied to simultaneously characterize the constituents in Suanzaoren tang. Analysis was performed on an Agilent Zorbax SB-C18， Rapid Resolution HT column(4.6 mm×50 mm，1.8 μm) with gradient elution of acetonitrile(A)-aqueous solution containing 0.05% formic acid(B) at a flow rate of 0.6 mL·min-1 and the column temperature was 30 ℃. By comparing MS fragmentation， accurate molecular weight， literature date and standard compounds information， a total of 48 compounds were successfully identified or speculated. The origins of these compounds were assigned to the corresponding Chinese medicine. Thirty-one compounds were reported in Suanzaoren tang for the first time. LC-Q-TOF-MS combined with LC-IT-MS is a simple and rapid tool for the identification of constituents of Suanzaoren tang， and the results could provide evidence for the research on quality combined and effective constituents of Suanzaoren tang.
[Key words] LC-Q-TOF-MS； LC-IT-MS； Suanzaoren tang； chemical costituents
doi：10.4268/cjcmm20140319
[责任编辑 孔晶晶], http://www.100md.com(朱云祥 陈璐琳 龚婧如 王书芳)