雷公藤内酯醇调控COX2/PGE2轴抑制Th17细胞分化的研究(4)
Guangzhou 510120， China； 2.Department of Gynecology， Second Affiliated Hospital of
Guangzhou University of Chinese Medicine， Guangzhou 510120， China)
[Abstract] Triptolide (TPT)， an active compound extracted from Chinese herb Tripterygium wilfordii ， has been used in therapy of rheumatoid arthritis (RA). In this study， after synovial fibroblasts from rheumatoid arthritis (RASFs) were treated with TPT， we investigated its effect on the differentiation of Th17 cells. Firstly， the mRNA level of cyclooxygenase (COX) wad detected by qRT-PCR and the protein level of prostaglandin E2 (PGE2) was tested by ELISA in RASFs treated with different concentrations (0， 10， 50， 100 nmol·L-1) of TPT. Then after TPT pre-treated RASFs and RA CD4+T cells were co-cultured for 3 days in the presence or absence of PGE2， IL-17 and IFN-γ production in CD4+T cell subsets were detected by flow cytometry. The results showed TPT decreased the mRNA experssion of COX2 and the secretion of PGE2 in RASFs in a dose-dependent manner(P<0.05). We further found that differentiation of Th17 cells was downregulated in a dose-dependent manner， and exogenous PGE2 could reverse the inhibition of Th17 cell differentiation(P<0.05). Taken together， our results demonstrated that TPT inhibited the mRNA level of COX2 and the secretion of PGE2 in RASFs， which partly led to impaired Th17 cell differentiation in vitro.
[Key words] triptolide； Th17 cells； prostaglandin E2； synovial fibroblasts
doi：10.4268/cjcmm20140334
[责任编辑 陈玲], 百拇医药(彭桉平 王小云 庄俊华)
Guangzhou University of Chinese Medicine， Guangzhou 510120， China)
[Abstract] Triptolide (TPT)， an active compound extracted from Chinese herb Tripterygium wilfordii ， has been used in therapy of rheumatoid arthritis (RA). In this study， after synovial fibroblasts from rheumatoid arthritis (RASFs) were treated with TPT， we investigated its effect on the differentiation of Th17 cells. Firstly， the mRNA level of cyclooxygenase (COX) wad detected by qRT-PCR and the protein level of prostaglandin E2 (PGE2) was tested by ELISA in RASFs treated with different concentrations (0， 10， 50， 100 nmol·L-1) of TPT. Then after TPT pre-treated RASFs and RA CD4+T cells were co-cultured for 3 days in the presence or absence of PGE2， IL-17 and IFN-γ production in CD4+T cell subsets were detected by flow cytometry. The results showed TPT decreased the mRNA experssion of COX2 and the secretion of PGE2 in RASFs in a dose-dependent manner(P<0.05). We further found that differentiation of Th17 cells was downregulated in a dose-dependent manner， and exogenous PGE2 could reverse the inhibition of Th17 cell differentiation(P<0.05). Taken together， our results demonstrated that TPT inhibited the mRNA level of COX2 and the secretion of PGE2 in RASFs， which partly led to impaired Th17 cell differentiation in vitro.
[Key words] triptolide； Th17 cells； prostaglandin E2； synovial fibroblasts
doi：10.4268/cjcmm20140334
[责任编辑 陈玲], 百拇医药(彭桉平 王小云 庄俊华)