人参皂苷Ro碱水解动力学研究及水解产物结构解析(4)
[9] 张毅，翁代群，王海军，等.乌头碱的水解动力学研究[J].中草药，2009，40(S1)：120.
[10] Peng W，Sun J，Lin F，et al.Facile synthesis of ginsenoside Ro[J].Synlett， 2004(2)：259.
[11] 邱楠楠.紫红参化学成分、指纹图谱及生物活性的研究[D].长春：吉林大学，2013.
[12] Rukunga G M，Waterman P G.A new oleanane glycoside from the stem bark of Albizia gummifera[J].Fitoterapia，2001，72(2)：140.
[13] Hasegawa H Fau-Matsumiya S，Matsumiya S Fau-Uchiyama M，Uchiyama M Fau-Kurokawa T，et al.Inhibitory effect of some triterpenoid saponins on glucose transport in tumor cells and its application to in vitro cytotoxic and antiviral activities[J].Planta Med，1994，60(3)：240.
[14] 曲正义，刘宏群，郑培和，等.齐墩果酸型皂苷药理活性研究[J].中成药，2012，34(6)：1143.
Study on hydrolysis kinetics of ginsenoside-Ro in alkaline medium and
structural analysis of its hydrolyzate
ZHU Qiong， LI De-kun， ZHOU Da-zheng， YE Zheng-liang， LIU Wen-yuan
(1.Department of Pharmaceutical Analysis， China Pharmaceutical University， Nanjing 210009， China；
2.Tianjin Tasly Pride Pharmaceutical Company Limited， Tianjin 300402， China)
[Abstract] The ginsenoside-Ro is sensitive to be hydrolyzed in an alkaline medium.This paper investigated the hydrolysis kinetics of ginsenoside-Ro under different pH and temperature values.The results showed ginsenoside-Ro in alkaline solution followed pseudo-first-order reaction.Hydrolysis kinetics of ginsenoside-Ro has not been reported previously.The hydrolysis rate was independent of initial concentration.On the basis of UFLC-MS/MS， NMR， as well as chemical evidence，the structure of hydrolyzate was assigned as 3-O-[β-D-glucuronopyranosyl-(1→2)-β-D-glucopyranosyl]-oleanolic acid.
[Key words] ginsenoside-Ro； hydrolysis kinetics； structural analysis； zingibroside-R1
doi：10.4268/cjcmm20140522
[责任编辑 孔晶晶], 百拇医药(朱琼 李德坤 周大铮 叶正良 柳文媛)
[10] Peng W，Sun J，Lin F，et al.Facile synthesis of ginsenoside Ro[J].Synlett， 2004(2)：259.
[11] 邱楠楠.紫红参化学成分、指纹图谱及生物活性的研究[D].长春：吉林大学，2013.
[12] Rukunga G M，Waterman P G.A new oleanane glycoside from the stem bark of Albizia gummifera[J].Fitoterapia，2001，72(2)：140.
[13] Hasegawa H Fau-Matsumiya S，Matsumiya S Fau-Uchiyama M，Uchiyama M Fau-Kurokawa T，et al.Inhibitory effect of some triterpenoid saponins on glucose transport in tumor cells and its application to in vitro cytotoxic and antiviral activities[J].Planta Med，1994，60(3)：240.
[14] 曲正义，刘宏群，郑培和，等.齐墩果酸型皂苷药理活性研究[J].中成药，2012，34(6)：1143.
Study on hydrolysis kinetics of ginsenoside-Ro in alkaline medium and
structural analysis of its hydrolyzate
ZHU Qiong， LI De-kun， ZHOU Da-zheng， YE Zheng-liang， LIU Wen-yuan
(1.Department of Pharmaceutical Analysis， China Pharmaceutical University， Nanjing 210009， China；
2.Tianjin Tasly Pride Pharmaceutical Company Limited， Tianjin 300402， China)
[Abstract] The ginsenoside-Ro is sensitive to be hydrolyzed in an alkaline medium.This paper investigated the hydrolysis kinetics of ginsenoside-Ro under different pH and temperature values.The results showed ginsenoside-Ro in alkaline solution followed pseudo-first-order reaction.Hydrolysis kinetics of ginsenoside-Ro has not been reported previously.The hydrolysis rate was independent of initial concentration.On the basis of UFLC-MS/MS， NMR， as well as chemical evidence，the structure of hydrolyzate was assigned as 3-O-[β-D-glucuronopyranosyl-(1→2)-β-D-glucopyranosyl]-oleanolic acid.
[Key words] ginsenoside-Ro； hydrolysis kinetics； structural analysis； zingibroside-R1
doi：10.4268/cjcmm20140522
[责任编辑 孔晶晶], 百拇医药(朱琼 李德坤 周大铮 叶正良 柳文媛)