姜黄素可通过内质网应激途径诱导乳腺癌细胞MDA—MB—231凋亡(3)
[5] Liu D，Chen Z. The effect of curcumin on breast cancer cells [J]. J Breast Cancer，2013，16(2)：133.
[6] White-Gilbertson S，Hua Y，Liu B. The role of endoplasmic reticulum stress in maintaining and targeting multiple myeloma：a double-edged sword of adaptation and apoptosis [J]. Front Genet，2013，11(4)：109.
[7] Scull C M，Tabas I. Mechanisms of ER stress-induced apoptosis in atherosclerosis[J]. Arterioscler Thromb Vasc Biol，2011，31(12)：2792.
Effect of curcumin in inducing apoptosis of MDA-MB-213
cells by activating endoplasmic reticulum stress
HONG Ri， WU Yong-qiang， WU Yue
(Diagnosis and Treatment Center of Mastopathy， the First Hospital Affiliated to Zhejiang University of
Traditional Chinese Medicine， Hangzhou 310006， China)
[Abstract] Objective： To explore the possible mechanism of curcumin in inducing the apoptosis of breast cancer cell MDA-MB-231. Method： Curcumin of different concentrations at 0，10 25，50，100，150，200 μmol·L-1 were used to intervene breast cancer cells MDA-MB-231 for 24 hours. MTT was used to observe its effect on the proliferation of breast cancer cells. The flow cytometry was used to detect its effect on the cell apoptosis. The real-time quantitative PCR and Western blot was used to assess the expression levels of GRP78 and CHOP in breast cancer cells. Result： Curcumin could inhibit the proliferative ability of breast cancer cells by inducing them in a concentration-dependent manner. Curcumin could significantly increase the expression levels of GRP78 and CHOP in breast cancer cells. Conclusion： Curcumin could induce the apoptosis of breast cancer cells MDA-MB-231 by activating endoplasmic reticulum stress.
[Key words] curcumin； breast cancer； endoplasmic reticulum stress
doi：10.4268/cjcmm20140826
[责任编辑 张宁宁], http://www.100md.com(洪日 吴永强 吴越)
[6] White-Gilbertson S，Hua Y，Liu B. The role of endoplasmic reticulum stress in maintaining and targeting multiple myeloma：a double-edged sword of adaptation and apoptosis [J]. Front Genet，2013，11(4)：109.
[7] Scull C M，Tabas I. Mechanisms of ER stress-induced apoptosis in atherosclerosis[J]. Arterioscler Thromb Vasc Biol，2011，31(12)：2792.
Effect of curcumin in inducing apoptosis of MDA-MB-213
cells by activating endoplasmic reticulum stress
HONG Ri， WU Yong-qiang， WU Yue
(Diagnosis and Treatment Center of Mastopathy， the First Hospital Affiliated to Zhejiang University of
Traditional Chinese Medicine， Hangzhou 310006， China)
[Abstract] Objective： To explore the possible mechanism of curcumin in inducing the apoptosis of breast cancer cell MDA-MB-231. Method： Curcumin of different concentrations at 0，10 25，50，100，150，200 μmol·L-1 were used to intervene breast cancer cells MDA-MB-231 for 24 hours. MTT was used to observe its effect on the proliferation of breast cancer cells. The flow cytometry was used to detect its effect on the cell apoptosis. The real-time quantitative PCR and Western blot was used to assess the expression levels of GRP78 and CHOP in breast cancer cells. Result： Curcumin could inhibit the proliferative ability of breast cancer cells by inducing them in a concentration-dependent manner. Curcumin could significantly increase the expression levels of GRP78 and CHOP in breast cancer cells. Conclusion： Curcumin could induce the apoptosis of breast cancer cells MDA-MB-231 by activating endoplasmic reticulum stress.
[Key words] curcumin； breast cancer； endoplasmic reticulum stress
doi：10.4268/cjcmm20140826
[责任编辑 张宁宁], http://www.100md.com(洪日 吴永强 吴越)