戊己丸活性代表成分在大鼠肝脏中分布的研究(5)
3.Guang′anmen Hospital， China Academy of Chinese Medical Sciences， Beijing 100053， China；
4.College of Chemistry & Environment protection Engineering， Southwest University for Nationalities， Chengdu 610041， China)
[Abstract] A L9(34) orthogonal design table to be used to get nine combinations of extraction of three herbs of Wuji pill： Coptis chinensis， Tetradium ruticarpum and Paeonia lactiflora Pall.，and nine extraction of single herbs correspondingly， altogether eighteen combinations. Quantification of five representative bioactive ingredients： berberine， palmatine， evodiamine， rutaecarpine， paeoniflorin in rat liver by ultra high liquid chromatography-tandem mass spectrometry after oral administration at 2 h time point of eighteen combinations. The result shows the bioactive ingredients have different concentrations betweem different combinations and the single herb with the same dosage significantly as well as the same dose combinations. C. chinensis with evodiamine concentration of low and high dose T. ruticarpum was positively correlated. T. ruticarpum with berberine concentration of low dose C. chinensis was negatively correlated and of meddle dose C. chinensis was correlated positively. T. ruticarpum with paeoniflorin concentration of middle dose P. lactiflora was correlated positively. P. lactiflora with palmatine concentration of middle dose C. chinensis was negatively correlated and with evodiamine and rutaecarpine concentration of middle dose T. ruticarpum was negatively correlated. These shows the three single herbs interactions resulted in the differences of each ingredients concentration in rat liver. The orthogonal analysis indicates the combination 12∶6∶6 make the maximum concentration in rat liver.
[Key words] Wuji pill； combinations； distribution in liver
doi：10.4268/cjcmm20140930, 百拇医药(张瑞杰 陈颖 巩仔鹏 董宇 张海先 杨庆 翁小刚 李玉洁 朱晓新)
4.College of Chemistry & Environment protection Engineering， Southwest University for Nationalities， Chengdu 610041， China)
[Abstract] A L9(34) orthogonal design table to be used to get nine combinations of extraction of three herbs of Wuji pill： Coptis chinensis， Tetradium ruticarpum and Paeonia lactiflora Pall.，and nine extraction of single herbs correspondingly， altogether eighteen combinations. Quantification of five representative bioactive ingredients： berberine， palmatine， evodiamine， rutaecarpine， paeoniflorin in rat liver by ultra high liquid chromatography-tandem mass spectrometry after oral administration at 2 h time point of eighteen combinations. The result shows the bioactive ingredients have different concentrations betweem different combinations and the single herb with the same dosage significantly as well as the same dose combinations. C. chinensis with evodiamine concentration of low and high dose T. ruticarpum was positively correlated. T. ruticarpum with berberine concentration of low dose C. chinensis was negatively correlated and of meddle dose C. chinensis was correlated positively. T. ruticarpum with paeoniflorin concentration of middle dose P. lactiflora was correlated positively. P. lactiflora with palmatine concentration of middle dose C. chinensis was negatively correlated and with evodiamine and rutaecarpine concentration of middle dose T. ruticarpum was negatively correlated. These shows the three single herbs interactions resulted in the differences of each ingredients concentration in rat liver. The orthogonal analysis indicates the combination 12∶6∶6 make the maximum concentration in rat liver.
[Key words] Wuji pill； combinations； distribution in liver
doi：10.4268/cjcmm20140930, 百拇医药(张瑞杰 陈颖 巩仔鹏 董宇 张海先 杨庆 翁小刚 李玉洁 朱晓新)