芍药苷人工抗原的合成与鉴定(3)
[13] 屈会化，赵琰，李翼飞，等. 中药小分子活性成分人工抗原合成的技术要点[J]. 中草药，2012，43(10)：1880.
[14] 张丽芳，李伟岭，薛飞群. 人工抗原的鉴定[J]. 畜牧与饲料科学，2009(10)：116.
Synthesis and identification of artificial antigens of paneoniflorin
QU Hui-hua， ZHAO Yan， SU Xin， HE Na-na， SUN Ye， KONG Hui， ZHAO Yan， WANG Qing-guo
(1.School of Basic Medical Sciences， Beijing University of Chinese Medicine， Beijing 100029 ， China；
2.School of Chinese Materia Medica， Beijing University of Chinese Medicine， Beijing 100102， China；
3.Center of Scientific Research， Beijing University of Chinese Medicine， Beijing 100029， China；
4.″Classical Prescription Application Foundation Research″ Innovation Team， Beijing University of
Chinese Medicine， Beijing 100029， China)
[Abstract] Oxidation method with sodium iodide was used to synthesize immunogenic antigen (PF-BSA) and coating antigen (PF-OVA) of paeoniflorin. UV spectroscopy showed that paeoniflorin was successfully conjugated with BSA and OVA. After immunized by PF-BSA， the mice can produce anti-paeoniflorin antibodies specifically. The ELISA test results showed the high titer (1∶12 800) and specificity ( IC50=0.791 mg·L-1 )of the antiserum from mice injected with PF-BSA. Also， the antiserum showed low cross activities against nine traditional Chinese medicine (TCM) of small molecules. These artificial antigens were successfully synthesized and the anti-paeoniflorin antibody well prepared， which provides the experimental basis for the further study of ELISA and its kit.
[Key words] paeoniflorin； artificial antigen； sodium periodate oxidation； immunoassay method； anti-paeoniflorin antibodies
doi：10.4268/cjcmm20141119
[责任编辑 孔晶晶], 百拇医药(屈会化 赵妍 苏歆 贺娜娜 孙晔 孔慧 赵琰 王庆国)
[14] 张丽芳，李伟岭，薛飞群. 人工抗原的鉴定[J]. 畜牧与饲料科学，2009(10)：116.
Synthesis and identification of artificial antigens of paneoniflorin
QU Hui-hua， ZHAO Yan， SU Xin， HE Na-na， SUN Ye， KONG Hui， ZHAO Yan， WANG Qing-guo
(1.School of Basic Medical Sciences， Beijing University of Chinese Medicine， Beijing 100029 ， China；
2.School of Chinese Materia Medica， Beijing University of Chinese Medicine， Beijing 100102， China；
3.Center of Scientific Research， Beijing University of Chinese Medicine， Beijing 100029， China；
4.″Classical Prescription Application Foundation Research″ Innovation Team， Beijing University of
Chinese Medicine， Beijing 100029， China)
[Abstract] Oxidation method with sodium iodide was used to synthesize immunogenic antigen (PF-BSA) and coating antigen (PF-OVA) of paeoniflorin. UV spectroscopy showed that paeoniflorin was successfully conjugated with BSA and OVA. After immunized by PF-BSA， the mice can produce anti-paeoniflorin antibodies specifically. The ELISA test results showed the high titer (1∶12 800) and specificity ( IC50=0.791 mg·L-1 )of the antiserum from mice injected with PF-BSA. Also， the antiserum showed low cross activities against nine traditional Chinese medicine (TCM) of small molecules. These artificial antigens were successfully synthesized and the anti-paeoniflorin antibody well prepared， which provides the experimental basis for the further study of ELISA and its kit.
[Key words] paeoniflorin； artificial antigen； sodium periodate oxidation； immunoassay method； anti-paeoniflorin antibodies
doi：10.4268/cjcmm20141119
[责任编辑 孔晶晶], 百拇医药(屈会化 赵妍 苏歆 贺娜娜 孙晔 孔慧 赵琰 王庆国)