基于ITS2条形码的中药材天南星及其混伪品DNA分子鉴定(3)
[19]Tamura K，Stecher G，Peterson D，et al. MEGA6： molecular evolutionary genetics analysis version 6.0[J]. Mol Biol Evol，2013，30： 2725.
[20]张丽春. 中药“虎掌”与“天南星”的本草学考证[J]. 兰州医学院学报，2002，28(3)：37.
[21]楼之岑. 常用中药材品种整理和质量研究. 第1册[M]. 北京：北京医科大学&中国协和医科大学联合出版社，1995.
DNA barcoding identification between Arisaematis Rhizoma and its
adulterants based on ITS2 sequences
SHI Lin-chun1，CHEN Jun1，XIANG Li2，SONG Jing-yuan1，YAO Hui1*
(1. Institute of Medicinal Plant Development，Chinese Academy of Medical Sciences，Peking Union Medical College，Beijing 100193，China；
2. Institute of Chinese Materia Medica，China Academy of Chinese Medical Sciences，Beijing 100700，China)
[Abstract] Fifty-eight samples belonging to 7 species of Arisaematis Rhizoma and its adulterants were collected. The ITS2 locus was employed as a DNA barcode and amplified，sequenced and assembled for all of the collected samples. Then，ITS2 sequences have been annotated using HMM-based method. The intra- and inter-specific variations were calculated and NJ tree was constructed using MEGA 6.0 software. The results showed that inter-specific K2P distances were significantly larger than intra-specific distances for all of the three origin species of Arisaematis Rhizoma. Furthermore，three origin species，Arisaema amurense， A. erubescens and A. heterophyllum，can be respectively formed to be a single branch with high bootstrap values. It is concluded that ITS2 can be used to correctly identify Arisaematis Rhizoma from its adulterants and the application of ITS2 in the identification of traditional Chinese medicine has an important prospective.
[Key words] Arisaematis Rhizoma；ITS2；DNA barcoding；identification
doi：10.4268/cjcmm20141205, http://www.100md.com(石林春 陈俊 向丽 宋经元 姚辉)
[20]张丽春. 中药“虎掌”与“天南星”的本草学考证[J]. 兰州医学院学报，2002，28(3)：37.
[21]楼之岑. 常用中药材品种整理和质量研究. 第1册[M]. 北京：北京医科大学&中国协和医科大学联合出版社，1995.
DNA barcoding identification between Arisaematis Rhizoma and its
adulterants based on ITS2 sequences
SHI Lin-chun1，CHEN Jun1，XIANG Li2，SONG Jing-yuan1，YAO Hui1*
(1. Institute of Medicinal Plant Development，Chinese Academy of Medical Sciences，Peking Union Medical College，Beijing 100193，China；
2. Institute of Chinese Materia Medica，China Academy of Chinese Medical Sciences，Beijing 100700，China)
[Abstract] Fifty-eight samples belonging to 7 species of Arisaematis Rhizoma and its adulterants were collected. The ITS2 locus was employed as a DNA barcode and amplified，sequenced and assembled for all of the collected samples. Then，ITS2 sequences have been annotated using HMM-based method. The intra- and inter-specific variations were calculated and NJ tree was constructed using MEGA 6.0 software. The results showed that inter-specific K2P distances were significantly larger than intra-specific distances for all of the three origin species of Arisaematis Rhizoma. Furthermore，three origin species，Arisaema amurense， A. erubescens and A. heterophyllum，can be respectively formed to be a single branch with high bootstrap values. It is concluded that ITS2 can be used to correctly identify Arisaematis Rhizoma from its adulterants and the application of ITS2 in the identification of traditional Chinese medicine has an important prospective.
[Key words] Arisaematis Rhizoma；ITS2；DNA barcoding；identification
doi：10.4268/cjcmm20141205, http://www.100md.com(石林春 陈俊 向丽 宋经元 姚辉)