白术与苍术及其混伪品DNA条形码鉴定研究(4)
2. Institute of Medicinal Plant Development，Chinese Academy of Medical Science & Peking Union Medical College，Beijing 100193，China；
3.Institute of Chinese Materia Medica，China Academy of Chinese Medical Sciences，Beijing 100700，China)
[Abstract] Atractylodis Macrocephalae Rhizoma and Atractylodis Rhizoma were widely used in strengthening spleen under different disease conditions，and were easily and often misused each other. Therefore，DNA barcode was used to distinguish Atractylodis Macrocephalae Rhizoma and Atractylodis Rhizoma from their adulterants to ensure the safe use.The sequence lengths of ITS2 of Atractylodes macrocephala，Atractylodis Rhizoma (A. lancea，A. japonica and A. coreana) were both 229 bp. Among the ITS2 sequences of A. macrocephala，only one G/C transversion was detected at site 98，and the average GC content was 69.42%. No variable site was detected in the ITS2 sequences of A. lancea. The maximum K2P intraspecific genetic distances of both A. japonica and A. coreana were 0.013. The maximum K2P intraspecific genetic distances of A. macrocephala，A. lancea，A. japonica and A. coreana were less than the minimum interspecific genetic distance of adulterants. The ITS2 sequences in each of these polytypic species were separated into pairs of divergent clusters in the NJ tree. DNA barcoding could be used as a fast and accurate identification method to distinguish Atractylodis Macrocephalae Rhizoma，Atractylodis Rhizoma，from their adulterants to ensure its safe use.
[Key words] Atractylodis Macrocephalae Rhizoma；Atractylodis Rhizoma；adulterants；DNA barcoding
doi：10.4268/cjcmm20141209, http://www.100md.com(余亚东 石林春 马晓冲 孙伟 叶萌 向丽)
3.Institute of Chinese Materia Medica，China Academy of Chinese Medical Sciences，Beijing 100700，China)
[Abstract] Atractylodis Macrocephalae Rhizoma and Atractylodis Rhizoma were widely used in strengthening spleen under different disease conditions，and were easily and often misused each other. Therefore，DNA barcode was used to distinguish Atractylodis Macrocephalae Rhizoma and Atractylodis Rhizoma from their adulterants to ensure the safe use.The sequence lengths of ITS2 of Atractylodes macrocephala，Atractylodis Rhizoma (A. lancea，A. japonica and A. coreana) were both 229 bp. Among the ITS2 sequences of A. macrocephala，only one G/C transversion was detected at site 98，and the average GC content was 69.42%. No variable site was detected in the ITS2 sequences of A. lancea. The maximum K2P intraspecific genetic distances of both A. japonica and A. coreana were 0.013. The maximum K2P intraspecific genetic distances of A. macrocephala，A. lancea，A. japonica and A. coreana were less than the minimum interspecific genetic distance of adulterants. The ITS2 sequences in each of these polytypic species were separated into pairs of divergent clusters in the NJ tree. DNA barcoding could be used as a fast and accurate identification method to distinguish Atractylodis Macrocephalae Rhizoma，Atractylodis Rhizoma，from their adulterants to ensure its safe use.
[Key words] Atractylodis Macrocephalae Rhizoma；Atractylodis Rhizoma；adulterants；DNA barcoding
doi：10.4268/cjcmm20141209, http://www.100md.com(余亚东 石林春 马晓冲 孙伟 叶萌 向丽)