高效液相质谱联用技术鉴定刺五加注射液中苯丙素类化学成分(5)
2. ZBD Pharmaceutical Company， Haerbin 150060， China)
[Abstract] There are reports about the chemical compounds of Ciwujia herbs， but with no study report about the chemical material basis of Ciwujia injection (CWJI). In this study， LC-MSn and LC-Q-TOF-MS techniques were adopted for a qualitative analysis on phenylpropanoids in CWJI. The UltmateTMXB-C18 column (4.6 mm×250 mm， 5 μm) was adopted and eluted with the mobile phase of 0.5% formic acid-water and acetonitrile， with the flow rate at 0.8 mL·min-1 and the column temperature at 20 ℃. Based on the data of high-resolution and multi-stage MS， control products and literatures， altogether 54 phenylpropanoids were identified in Ciwujia Injection， including 34 phenylpropanoids， 16 ligans and 4 coumarins. Among them， 28 were reported for the first time in Ciwujia， and 14 compound structures were identified in comparison with the control products. The method established in this study could be used to simply and rapidly identify phenylpropanoids in CWJI. The findings provide scientific data for defining the chemical material basis of CWJI.
[Key words] Ciwujia injection； LC-MSn； LC-Q-TOF-MS； phenylpropanoids
doi：10.4268/cjcmm20141331
[责任编辑 马超一], http://www.100md.com(黄婧 邵青 项彦华 葛志伟 范骁辉)
[Abstract] There are reports about the chemical compounds of Ciwujia herbs， but with no study report about the chemical material basis of Ciwujia injection (CWJI). In this study， LC-MSn and LC-Q-TOF-MS techniques were adopted for a qualitative analysis on phenylpropanoids in CWJI. The UltmateTMXB-C18 column (4.6 mm×250 mm， 5 μm) was adopted and eluted with the mobile phase of 0.5% formic acid-water and acetonitrile， with the flow rate at 0.8 mL·min-1 and the column temperature at 20 ℃. Based on the data of high-resolution and multi-stage MS， control products and literatures， altogether 54 phenylpropanoids were identified in Ciwujia Injection， including 34 phenylpropanoids， 16 ligans and 4 coumarins. Among them， 28 were reported for the first time in Ciwujia， and 14 compound structures were identified in comparison with the control products. The method established in this study could be used to simply and rapidly identify phenylpropanoids in CWJI. The findings provide scientific data for defining the chemical material basis of CWJI.
[Key words] Ciwujia injection； LC-MSn； LC-Q-TOF-MS； phenylpropanoids
doi：10.4268/cjcmm20141331
[责任编辑 马超一], http://www.100md.com(黄婧 邵青 项彦华 葛志伟 范骁辉)