大鼠口服芍苓消银片后血液中芍药苷?落新妇苷?迷迭香酸?异嗪皮啶?甘草苷的药物动力学研究(3)
[Abstract] To establish a method for the determination of astilbin， peoniflorin， rasmarinci acid， isofraxidin and liquiritin contained in Shaolin Xiaoyin tablets， in order to lay a foundation for designing late-stage dosage forms and clinical medication schemes. In this paper， efforts were made to establish a method for the determination of the blood concentration of the five components and study the in vivo pharmacokinetics in rats. The blood concentration was determined by HPLC. Phenomenex C18 column (4.6 mm×250 mm， 5 μm) was adopted and eluted with methanol-acetonitrile-0.05% formic acid， the flow rate was 0.8 mL·min-1， and the wavelength was 275 nm. The samples were processed by the solid phase extraction method. After oral administration of Shaoling Xiaoyin tablets， the rat bloods were collected at different time points to determine the blood concentrations. The experimental results showed that the baseline separation could be adopted for the five components， and astilbin， peoniflorin， rasmarinci acid， isofraxidin and liquiritin showed good linear relations within ranges of 2.48-248， 0.213 6-21.36， 0.531-53.1，0.704-70.4， 0.253-25.3 mg·L-1. All the five components could be absorbed in blood and excreted quickly. The method established in this paper is rapid and accurate， and could be used for in vivo analysis on preparations containing similar components. The main components in Shaoling Xiaoyin tablets could be absorbed and excreted quickly， and thus suitable to be made into sustained release tablets. Common preparations are required to be taken for 4-6 times a day.
[Key words] peoniflorin； isofraxidin； astilbin； rosmarinci acid； liquiritin； Shaoling Xiaoyin tablets； pharmacokinetics
doi：10.4268/cjcmm20141339
[责任编辑 陈玲], http://www.100md.com(赵瑞芝 王银洁 冯丽敏 卢传坚)
[Key words] peoniflorin； isofraxidin； astilbin； rosmarinci acid； liquiritin； Shaoling Xiaoyin tablets； pharmacokinetics
doi：10.4268/cjcmm20141339
[责任编辑 陈玲], http://www.100md.com(赵瑞芝 王银洁 冯丽敏 卢传坚)