水翁花黄酮DMC诱导肝癌细胞SMMC—7721凋亡的机制研究(4)
cells by DMC， a chalcone from buds of Cleistocalyx operculatus
YE Chun-lin， LAI Yi-feng， LIU Xuan-gan， HUANG Qi
(School of Biological and Chemical Engineering， Zhejiang University of Science and Technology， Hangzhou 310023， China)
[Abstract] Objective： To study the in-vitro inducing apoptosis mechanism of human hepatoma SMMC-7721 cells by 2′，4′-dihydroxy-6′-methoxy-3′，5′-dimethylchalcone (DMC)， a chalcone compound from Cleistocalyx operculatus. Method： Quantitative DNA fragmentation assay was carried out to detect the effect of DMC of different concentrations on SMMC-7721 cells，according to the method of Sellins and Cohen with some modifications. Telomerase activities of the cells were determined by PCR-ELISA methods. The expression quantity of c-myc and hTERT mRNA were determined by semi-quantitative RT-PCR. The effect of DMC on expression levels of cmyc and hTERT protein were measured by western blot. Result： The percentage of DNA fragmentation increased with notable concentration dependence， after treatment with DMC for 48 h. Compared with that of control group， the telomerase activity of the cells decreased by (66.2±2.1)% after 48 h treatment with 20 μmol·L-1 DMC， the mRNA expression of c-myc and hTERT decreased by (67.3±2.1)% and (64.4±2.3)%， respectively， and the protein expression of c-myc and hTERT decreased by (69.6±1.9)% and (71.3±2.4)%， respectively. Conclusion： DMC can induce SMMC-7721 cell apoptosis and the apoptosis mechanism may be related to the decreased mRNA and protein expression of c-myc and hTERT.
[Key words] Cleistocalyx operculatus； apoptosis； flavonoids； tumor； telomerase
doi：10.4268/cjcmm20141528
[责任编辑 张宁宁], http://www.100md.com(叶春林 赖依峰 刘宣淦 黄琦)
YE Chun-lin， LAI Yi-feng， LIU Xuan-gan， HUANG Qi
(School of Biological and Chemical Engineering， Zhejiang University of Science and Technology， Hangzhou 310023， China)
[Abstract] Objective： To study the in-vitro inducing apoptosis mechanism of human hepatoma SMMC-7721 cells by 2′，4′-dihydroxy-6′-methoxy-3′，5′-dimethylchalcone (DMC)， a chalcone compound from Cleistocalyx operculatus. Method： Quantitative DNA fragmentation assay was carried out to detect the effect of DMC of different concentrations on SMMC-7721 cells，according to the method of Sellins and Cohen with some modifications. Telomerase activities of the cells were determined by PCR-ELISA methods. The expression quantity of c-myc and hTERT mRNA were determined by semi-quantitative RT-PCR. The effect of DMC on expression levels of cmyc and hTERT protein were measured by western blot. Result： The percentage of DNA fragmentation increased with notable concentration dependence， after treatment with DMC for 48 h. Compared with that of control group， the telomerase activity of the cells decreased by (66.2±2.1)% after 48 h treatment with 20 μmol·L-1 DMC， the mRNA expression of c-myc and hTERT decreased by (67.3±2.1)% and (64.4±2.3)%， respectively， and the protein expression of c-myc and hTERT decreased by (69.6±1.9)% and (71.3±2.4)%， respectively. Conclusion： DMC can induce SMMC-7721 cell apoptosis and the apoptosis mechanism may be related to the decreased mRNA and protein expression of c-myc and hTERT.
[Key words] Cleistocalyx operculatus； apoptosis； flavonoids； tumor； telomerase
doi：10.4268/cjcmm20141528
[责任编辑 张宁宁], http://www.100md.com(叶春林 赖依峰 刘宣淦 黄琦)