黄芩苷对鱼藤酮致PC12细胞损伤的保护作用研究(4)
Protective effect of baicalin against rotenone induced injury on PC12 cells
JI Hai-jie， TONG Li-guo， BAI Chong-zhi， SONG Mei-qing， CHEN Nai-hong， FENG Ma-li
(1.Shanxi Province Academy of Traditional Chinese Medicine， Taiyuan 030012， China；
2.Institute of Materia Medica， Chinese Academy of Medical Sciences & Peking Union Medical College， Beijing 100050， China)
[Abstract] Objective： To explore the protective effect of baicalin against rotenone-induced injury on PC12 cells， and the potential mechanism of action was also explored. Method： PC12 cells were injured by rotenone and were treated with different concentrations (0.1， 1， 10 μmol·L-1) of baicalin at the same time. Cell viability was analyzed by MTT， and morphology was observed by phase-contrast microscopy. The cell apoptosis was detected by flow cytometry by Annexin V-FITC/PI staining. The intracellular ROS level was determined by fluorescence microscope with DCF-DA staining. The expression of Bcl-2， Bax and Caspase-3 was analyzed by Western blot. Result： The viability of PC12 cells exposure to rotenone for 24 hour was gradually decreased with dose escalating and 1.5 μmol·L-1 was adopted to do the following experiment. Baicalin increased cell viability， improved cell morphology and decreased intracellular ROS level. Moreover， FACS indicated baicalin attenuated the apoptosis induced by rotenone significantly. Western blot showed that Bcl-2， Bax and Caspase-3 expression in rotenone-induced PC12 cells was reversed by baicalin. Conclusion： This study has demonstrated that baicalin protects PC12 cells against rotenone-induced apoptosis， at least in part， by scavenging excessive ROS and inhibiting the mitochondrion-dependent apoptotic pathway.
[Key words] PC12 cells； Parkinson′s disease； rotenone； baicalin
doi：10.4268/cjcmm20141529
[责任编辑 张宁宁], http://www.100md.com(吉海杰 仝立国 白崇智 宋美卿 陈乃宏 冯玛莉)
JI Hai-jie， TONG Li-guo， BAI Chong-zhi， SONG Mei-qing， CHEN Nai-hong， FENG Ma-li
(1.Shanxi Province Academy of Traditional Chinese Medicine， Taiyuan 030012， China；
2.Institute of Materia Medica， Chinese Academy of Medical Sciences & Peking Union Medical College， Beijing 100050， China)
[Abstract] Objective： To explore the protective effect of baicalin against rotenone-induced injury on PC12 cells， and the potential mechanism of action was also explored. Method： PC12 cells were injured by rotenone and were treated with different concentrations (0.1， 1， 10 μmol·L-1) of baicalin at the same time. Cell viability was analyzed by MTT， and morphology was observed by phase-contrast microscopy. The cell apoptosis was detected by flow cytometry by Annexin V-FITC/PI staining. The intracellular ROS level was determined by fluorescence microscope with DCF-DA staining. The expression of Bcl-2， Bax and Caspase-3 was analyzed by Western blot. Result： The viability of PC12 cells exposure to rotenone for 24 hour was gradually decreased with dose escalating and 1.5 μmol·L-1 was adopted to do the following experiment. Baicalin increased cell viability， improved cell morphology and decreased intracellular ROS level. Moreover， FACS indicated baicalin attenuated the apoptosis induced by rotenone significantly. Western blot showed that Bcl-2， Bax and Caspase-3 expression in rotenone-induced PC12 cells was reversed by baicalin. Conclusion： This study has demonstrated that baicalin protects PC12 cells against rotenone-induced apoptosis， at least in part， by scavenging excessive ROS and inhibiting the mitochondrion-dependent apoptotic pathway.
[Key words] PC12 cells； Parkinson′s disease； rotenone； baicalin
doi：10.4268/cjcmm20141529
[责任编辑 张宁宁], http://www.100md.com(吉海杰 仝立国 白崇智 宋美卿 陈乃宏 冯玛莉)