鼠尾草酚抗乳腺癌细胞增殖活性及其雌激素受体亚型介导和调节机制的研究(4)
ZHAO Pi-wen1， 2， David Yue-Wei LEE2， Zhong-ze MA 2， SUN Yan-ling1，TAO Shi-ying1， ZANG Jin-feng 1， NIU Jian-zhao1*
(1. School of Preclinical Medicine， Beijing University of Chinese Medicine， Beijing 100029， China；
2. Mclean Hospital， Harvard Medical School， Belmont MA 02478， USA)
[Abstract]Carnosol has been proved to have anti-breast cancer effect in previous research. But its ER subtype′s specific regulation and mediation mechanisms remain unclear. The aim of this study is to observe the effect of carnosol on cell proliferation and its estrogen receptor α and β′s specific regulation and mediation mechanisms with ER positive breast cancer T47D cell. With estrogen receptor α and β antagonists MPP and PHTPP as tools， the MTT cell proliferation assay was performed to observe the effect of carnosol on T47D cell proliferation. The changes in the T47D cell proliferation cycle were detected by flow cytometry. The effect of carnosol on ERα and ERβ expressions of T47D cells was measured by Western blot. The findings showed that 1×10-5-1×10-7 mol·L-1 carnosol could significantly inhibit the T47D cell proliferation， which could be enhanced by MPP or weakened by PHTPP. Meanwhile， 1×10-5 mol·L-1 or 1×10-6 mol·L-1 carnosol could significantly increase ERα and ERβ expressions of T47D cells， and remarkably increase ERα/ERβ ratio. The results showed that carnosol showed the inhibitory effect on the proliferation of ER positive breast cancer cells through target cell ER， especially ERβ pathway. In the meantime， carnosol could regulate expressions and proportions of target cell ER subtype ERα and ERβ.
[Key words]carnosol； breast cancer； T47D cell； cell proliferation； cell cycle； estrogen receptor
doi：10.4268/cjcmm20141727
[责任编辑 张宁宁], 百拇医药(赵丕文 David Yue-Wei LEE Zhongze Ma 孙艳玲 陶仕英)
(1. School of Preclinical Medicine， Beijing University of Chinese Medicine， Beijing 100029， China；
2. Mclean Hospital， Harvard Medical School， Belmont MA 02478， USA)
[Abstract]Carnosol has been proved to have anti-breast cancer effect in previous research. But its ER subtype′s specific regulation and mediation mechanisms remain unclear. The aim of this study is to observe the effect of carnosol on cell proliferation and its estrogen receptor α and β′s specific regulation and mediation mechanisms with ER positive breast cancer T47D cell. With estrogen receptor α and β antagonists MPP and PHTPP as tools， the MTT cell proliferation assay was performed to observe the effect of carnosol on T47D cell proliferation. The changes in the T47D cell proliferation cycle were detected by flow cytometry. The effect of carnosol on ERα and ERβ expressions of T47D cells was measured by Western blot. The findings showed that 1×10-5-1×10-7 mol·L-1 carnosol could significantly inhibit the T47D cell proliferation， which could be enhanced by MPP or weakened by PHTPP. Meanwhile， 1×10-5 mol·L-1 or 1×10-6 mol·L-1 carnosol could significantly increase ERα and ERβ expressions of T47D cells， and remarkably increase ERα/ERβ ratio. The results showed that carnosol showed the inhibitory effect on the proliferation of ER positive breast cancer cells through target cell ER， especially ERβ pathway. In the meantime， carnosol could regulate expressions and proportions of target cell ER subtype ERα and ERβ.
[Key words]carnosol； breast cancer； T47D cell； cell proliferation； cell cycle； estrogen receptor
doi：10.4268/cjcmm20141727
[责任编辑 张宁宁], 百拇医药(赵丕文 David Yue-Wei LEE Zhongze Ma 孙艳玲 陶仕英)