2.5 Western blotting检测MeCP2，SFRP2蛋白表达

    表1 Real time qPCR引物序列

    Table 1 The sequence of the Real time qPCR primer

    基因引物序列(5′-3′)

    SFRP2F：GGAGACAAAGAGCAAGACCATT;R：GACCAGATAGGGAGCGTTGAT

    MeCP2F：GCAAAGCAGAGACATCAGAAAG;R：AGGTGGGGTCATCATACATAGG

    β-actinF：CCCATCTATGAGGGTTACGC;R：TTTAATGTCACGC ACGATTTC

    表2 MeCP2 siRNA干扰序列

    Table 2 The sequence of the MeCP2 siRNA

    siRNA 引物序列(5′-3′)

    MeCP2 F：GGGACCUAUGUAUGAUGACTT;R：GUCAUCAUACAUAGGUCCCTT

    Scrambled F：UUCUCCGAACGUGUCACGUTT;R：ACGUGACACG UUCGGAGAATT

    Western blotting裂解液充分裂解滑膜组织 ......