珍珠菜中黄酮苷类成分研究(1)
[摘要]研究珍珠菜Lysimachia clethroides的黄酮苷类成分。珍珠菜乙醇提取物经硅胶、Sephadex LH-20和Pre-HPLC等色谱技术分离纯化，通过质谱和核磁共振等波谱学方法鉴定化合物的结构。从珍珠菜中分离得到11个黄酮苷类化合物，分别鉴定为紫云英苷(1)，异槲皮苷(2)，异鼠李素-3-O-β-D-吡喃葡萄糖苷(3)，槲皮素-3-O-β-D-6″-乙酰基吡喃葡萄糖苷(4)，槲皮素-7-O-β-D-吡喃葡萄糖苷(5)，江户樱花苷(6)，2-羟基-柚皮素-5-O-β-D-吡喃葡萄糖苷(7)，山柰酚-3-O-芸香糖苷(8)，山柰酚-3-O-洋槐糖苷(9)，芦丁(10)，山柰酚-3，7-二-O-β-D-吡喃葡萄糖苷(11)。化合物4，7和11为首次从该属植物中分离得到，化合物3、 5和9为首次从该种植物中分离得到。体外活性测试结果显示化合物2，6，8具有一定的醛糖还原酶抑制活性，IC50分别为2.69，1.00，1.80 μmol·L-1;化合物1～11对5种人肿瘤细胞均没有明显的细胞毒活性(IC50 > 10 μmol·L-1)。
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[关键词]珍珠菜;黄酮苷;醛糖还原酶抑制活性;细胞毒活性
[收稿日期]2014-10-07
[基金项目]教育部创新团队项目(IRT1007);广西自然科学基金项目(2014GXNSFBA118042);广西高校科学技术研究项目(ZD2014024);广西师范大学校重点项目(2013ZD001)
[通信作者]*于德泉，研究方向为天然药物化学，Tel：(010)63165224，E-mail：dqyu@imm.ac.cn
Flavonol glycosides from Lysimachia clethroides
LIANG Dong1，2， LIU Yan-fei1， HAO Zhi-you1， LUO Huan1， WANG Yan1， ZHANG Chun-lei1，ZHANG Qing-jian1， CHEN Ruo-yun1， YU De-quan1*
, 百拇医药
(1. State Key Laboratory of Bioactive Substances and Functions of Natural Medicines，Institute of Materia Medica， Chinese Academy of Medical Sciences & Peking Union Medical College， Beijing 100050， China;
2. State Key Laboratory Cultivation Base for the Chemistry and Molecular Engineering of Medicinal Resources，School of Chemistry and Pharmaceutical Sciences， Guangxi Normal University， Guilin 541004， China)
[Abstract]Eleven flavonol glycosides were isolated from the ethanol extract of Lysimachia clethroides by a combination of various chromatographic techniques including column chromatography over silica gel， Sephadex LH-20， and reversed-phase HPLC. Their structures were identified as astragalin(1)， isoquercitrin(2)， isorhamnetin-3-O-β-D-glucopyranoside(3)， quercetin-3-O-β-D-6″-acetylglucopyranoside(4)， quercetin-7-O-β-D-glucopyranoside(5)， prunin(6)， 2-hydroxynaringin-5-O-β-D-glucopyranoside(7)， kaempferol-3-O-rutinonoside(8)， kaempferol-3-O-robinobioside(9)， rutin(10) and kaempferol-3，7-di-O-β-D-glucopyranoside(11). Among them， compounds 4， 7 and 11 were obtained from the Lysimachia genus for the first time， while compounds 3， 5 and 9 were firstly reported from this plant. In the preliminary assays， compounds 2， 6 and 8 possessed significant inhibition against aldose reductase， with IC50 values of 2.69， 1.00，1.80 μmol·L-1， respectively; none of compounds 1-11 exhibited obvious cytotoxic activity(IC50 > 10 μmol·L-1)., 百拇医药(梁东 刘彦飞 郝志友 罗桓 王艳 张春磊 张庆建 陈若芸 于德泉)
, http://www.100md.com
[关键词]珍珠菜;黄酮苷;醛糖还原酶抑制活性;细胞毒活性
[收稿日期]2014-10-07
[基金项目]教育部创新团队项目(IRT1007);广西自然科学基金项目(2014GXNSFBA118042);广西高校科学技术研究项目(ZD2014024);广西师范大学校重点项目(2013ZD001)
[通信作者]*于德泉，研究方向为天然药物化学，Tel：(010)63165224，E-mail：dqyu@imm.ac.cn
Flavonol glycosides from Lysimachia clethroides
LIANG Dong1，2， LIU Yan-fei1， HAO Zhi-you1， LUO Huan1， WANG Yan1， ZHANG Chun-lei1，ZHANG Qing-jian1， CHEN Ruo-yun1， YU De-quan1*
, 百拇医药
(1. State Key Laboratory of Bioactive Substances and Functions of Natural Medicines，Institute of Materia Medica， Chinese Academy of Medical Sciences & Peking Union Medical College， Beijing 100050， China;
2. State Key Laboratory Cultivation Base for the Chemistry and Molecular Engineering of Medicinal Resources，School of Chemistry and Pharmaceutical Sciences， Guangxi Normal University， Guilin 541004， China)
[Abstract]Eleven flavonol glycosides were isolated from the ethanol extract of Lysimachia clethroides by a combination of various chromatographic techniques including column chromatography over silica gel， Sephadex LH-20， and reversed-phase HPLC. Their structures were identified as astragalin(1)， isoquercitrin(2)， isorhamnetin-3-O-β-D-glucopyranoside(3)， quercetin-3-O-β-D-6″-acetylglucopyranoside(4)， quercetin-7-O-β-D-glucopyranoside(5)， prunin(6)， 2-hydroxynaringin-5-O-β-D-glucopyranoside(7)， kaempferol-3-O-rutinonoside(8)， kaempferol-3-O-robinobioside(9)， rutin(10) and kaempferol-3，7-di-O-β-D-glucopyranoside(11). Among them， compounds 4， 7 and 11 were obtained from the Lysimachia genus for the first time， while compounds 3， 5 and 9 were firstly reported from this plant. In the preliminary assays， compounds 2， 6 and 8 possessed significant inhibition against aldose reductase， with IC50 values of 2.69， 1.00，1.80 μmol·L-1， respectively; none of compounds 1-11 exhibited obvious cytotoxic activity(IC50 > 10 μmol·L-1)., 百拇医药(梁东 刘彦飞 郝志友 罗桓 王艳 张春磊 张庆建 陈若芸 于德泉)