主成分分析用于清肺消痤凝胶体外经皮渗透的研究(1)
[摘要]该文运用主成分分析法建立一种中药复方透皮给药的评价方法，并以此方法优选清肺消痤凝胶的最佳透皮吸收促进剂。实验中以离体小鼠腹部皮肤为透皮屏障，采用改良Franz扩散池装置进行透皮吸收试验，HPLC建立接受液的指纹图谱，确定共有峰，求出各共有峰的面积，应用主成分分析法求得各组不同时间点的总因子得分，并以各因子得分代替药物浓度求得药物累积释放量Q12，算出增渗倍数ER，以此比较各促进剂对药物的透皮促进作用。结果显示，与空白组相比，各促进剂均对药物有促进作用，而以2.5%氮酮+2.5%薄荷醇混合使用时的促进作用最强。主成分分析法可以较全面的反应接受液中药物的信息，以此建立的评价方法可准确的优选出透皮吸收促进剂，并为后续中药复方经皮给药提供了可借鉴的研究思路。
[关键词]中药复方;经皮吸收;主成分分析;透皮促进剂
In vitro transdermal delivery of Qingfei Xiaocuo gel based on
, 百拇医药
principal component analysis
REN Wei-gao， PENG Lin-xiu， LEI Fei-fei， SUN Cheng-xiang， PAN Jin-huo*
(Nanjing University of Chinese Medicine， Nanjing 210023， China)
[Abstract]The objective of the present study was to establish a method based on principal component analysis (PCA) for the study of transdermal delivery of Chinese medicinal formulae， and to choose the best penetration enhancers for Qingfei Xiaocuo gel depend on this method. Using improved Franz type diffusion cell and excised rat skin in vitro as transdermal barrier， the receptive solution fingerprint was established by HPLC， harvesting the areas of the common peaks in the fingerprint， then the total factor scores of the concentrations at different times were calculated using PCA and were employed instead of the concentrations to compute the cumulative amounts(Q12) and enhancement ratio (ER)， the latter of which were considered as the indexes for optimizing penetration enhancers. Compare to the control group， the ER of the other groups increased significantly and furthermore， 2.5% azone with 2.5% menthol manifested the best effect. PCA represent most information in the receptive solution， the method above could choose the best penetration enhancers， it could be a reference for the study of transdermal delivery of Chinese medicinal formulae.
, 百拇医药
[Key words]Chinese medicinal formulae; transdermal delivery; principal component analysis; penetration enhancers
doi：10.4268/cjcmm20150213
清肺消痤凝胶处方最早源自于《医宗金鉴》之“颠倒散”，在长期的临床实践中经化裁而成。本方主要由大黄、黄芩、硫磺、桑白皮等药味组成，具有清肺解毒、化湿消肿、祛瘀散结的功效，主治肺经血热及湿热瘀滞之痤疮。本方利用现代药剂学方法，开发成经皮给药新剂型——清肺消痤凝胶，充分发挥经皮给药系统用药方便、减少给药次数的优势，并可以避免肝脏首过效应，提供可预订的和较长的给药时间，维持稳定持久的血药浓度，降低药物的毒性和副作用[1]。
目前常用的促进药物透过角质层屏障的方法是使用透皮吸收促进剂，然而，现在大多数学者在研究促进剂对于中药经皮给药制剂透皮促进作用时，常常选择某一个或几个指标成分作为指标[2-3]，而促进剂对于不同中药成分的促进作用往往不一，甚至相反，简单的加和难以有说服力。本文运用主成分分析法对于中药复方经皮给药建立评价方法，利用其对所测得的多个中药组分进行综合评价，以消除各指标权重的不确定性及某些指标间存在的相关性将给综合评价带来的困难[4]。并将此方法用于清肺消痤凝胶透皮吸收研究中来，为后续中药经皮给药的研究提供可借鉴的方法。, http://www.100md.com(任卫高 彭琳秀 雷飞飞 孙成祥 潘金火)
[关键词]中药复方;经皮吸收;主成分分析;透皮促进剂
In vitro transdermal delivery of Qingfei Xiaocuo gel based on
, 百拇医药
principal component analysis
REN Wei-gao， PENG Lin-xiu， LEI Fei-fei， SUN Cheng-xiang， PAN Jin-huo*
(Nanjing University of Chinese Medicine， Nanjing 210023， China)
[Abstract]The objective of the present study was to establish a method based on principal component analysis (PCA) for the study of transdermal delivery of Chinese medicinal formulae， and to choose the best penetration enhancers for Qingfei Xiaocuo gel depend on this method. Using improved Franz type diffusion cell and excised rat skin in vitro as transdermal barrier， the receptive solution fingerprint was established by HPLC， harvesting the areas of the common peaks in the fingerprint， then the total factor scores of the concentrations at different times were calculated using PCA and were employed instead of the concentrations to compute the cumulative amounts(Q12) and enhancement ratio (ER)， the latter of which were considered as the indexes for optimizing penetration enhancers. Compare to the control group， the ER of the other groups increased significantly and furthermore， 2.5% azone with 2.5% menthol manifested the best effect. PCA represent most information in the receptive solution， the method above could choose the best penetration enhancers， it could be a reference for the study of transdermal delivery of Chinese medicinal formulae.
, 百拇医药
[Key words]Chinese medicinal formulae; transdermal delivery; principal component analysis; penetration enhancers
doi：10.4268/cjcmm20150213
清肺消痤凝胶处方最早源自于《医宗金鉴》之“颠倒散”，在长期的临床实践中经化裁而成。本方主要由大黄、黄芩、硫磺、桑白皮等药味组成，具有清肺解毒、化湿消肿、祛瘀散结的功效，主治肺经血热及湿热瘀滞之痤疮。本方利用现代药剂学方法，开发成经皮给药新剂型——清肺消痤凝胶，充分发挥经皮给药系统用药方便、减少给药次数的优势，并可以避免肝脏首过效应，提供可预订的和较长的给药时间，维持稳定持久的血药浓度，降低药物的毒性和副作用[1]。
目前常用的促进药物透过角质层屏障的方法是使用透皮吸收促进剂，然而，现在大多数学者在研究促进剂对于中药经皮给药制剂透皮促进作用时，常常选择某一个或几个指标成分作为指标[2-3]，而促进剂对于不同中药成分的促进作用往往不一，甚至相反，简单的加和难以有说服力。本文运用主成分分析法对于中药复方经皮给药建立评价方法，利用其对所测得的多个中药组分进行综合评价，以消除各指标权重的不确定性及某些指标间存在的相关性将给综合评价带来的困难[4]。并将此方法用于清肺消痤凝胶透皮吸收研究中来，为后续中药经皮给药的研究提供可借鉴的方法。, http://www.100md.com(任卫高 彭琳秀 雷飞飞 孙成祥 潘金火)