白芍饮片的化学成分测定及质量评价(1)
[摘要]目的：选择芍药苷、芍药内酯苷、苯甲酰芍药苷和丹皮酚为白芍饮片的含量测定指标，通过建立UPLC测定方法对其含量进行测定，以实现白芍饮片的质量评价。方法：采用ACQUITY UPLC HSS T3色谱柱，以乙腈- 0.05%磷酸水溶液为流动相进行梯度洗脱，柱温30 ℃，检测波长230 nm，流速0.4 mL·min-1。通过主成分分析法获得线性方程，利用主成分得分综合评价白芍饮片的质量。结果：在所建立的色谱条件下，芍药苷、芍药内酯苷、苯甲酰芍药苷、丹皮酚与其他组分能够达到良好分离，专属性、精密度、重复性、线性关系、加样回收、稳定性试验均符合中药质量分析要求。25批白芍饮片样品中有9批不符合2010年版《中国药典》白芍含量测定项下规定。经主成分综合评分法评定，样品中浙江产白芍饮片质量较佳，其次为安徽产、山东产白芍饮片。结论：本方法可用于测定白芍饮片中芍药苷、芍药内酯苷、苯甲酰芍药苷和丹皮酚的含量，并对白芍饮片进行质量评价，为白芍的质量控制提供了更快速、全面的方法。
[关键词]白芍;芍药苷;芍药内酯苷;苯甲酰芍药苷;丹皮酚;UPLC;含量测定;主成分综合评分;质量评价
, http://www.100md.com
[收稿日期]2014-10-15
[通信作者]*郭巧生，Tel：(025)84395980，E-mail：gqs@njau.edu.cn;*叶正良，研究员，Tel：(022)86342066，E-mail：yezl@yahoo.com
[作者简介]金林，硕士研究生，Tel：18105167378，E-mail：2012104202@njau.edu.cnDetermination of chemical components of Paeoniae Radix
Alba decoction pieces and its quality evaluationJIN Lin1， ZHAO Wan-shun2， GUO Qiao-sheng1*， YE Zheng-liang3* ， ZHANG Wen-sheng2
, http://www.100md.com (1. Institute of Chinese Medicinal Materials， Nanjing Agricultural University， Nanjing 210095， China;
2. Tasly Pharmaceutical Group Company Limited， Tianjin 300402， China;
3.Tasly Holding Group Company Limited， Tianjin 300402， China)[Abstract]Objective： An UPLC method was developed to evaluate the quality of Paeoniae Radix by simultaneously determining four components， paeoniflorin， albiflorin， benzoylpaeoniflorin and paeonol in Paeoniae Radix Alba decoction pieces. Method： The UPLC chromatographic column was ACQUITY UPLC HSS T3. The mobile phase was acetonitrile-0.05%phosphoric acid water with gradient elution. The column temperature was 30 ℃ and detection wavelength was 230 nm with a flow rate of 0.4 mL·min-1. A linear model was obtained through principal component analysis (PCA)， and PCA scores were used to evaluate the quality of Radix Paeoniae Alba decoction pieces comprehensively. Result： Paeoniflorin， albiflorin， benzoylpaeoniflorin and paeonol could be well separated from other components， and the results of specificity， precision， repeatability， linearity， recovery rate and stability reached the standards， respectively. The content of paeoniflorin in 9 batches of Paeoniae Radix Alba decoction pieces was below the standard given by Chinese Pharmacopoeia (2010 edition). Using the comprehensive scoring method with principal component analysis， the results showed that the samples from Zhejiang province have better quality than those from Anhui and Shandong provinces. Conclusion： The method established in this study can effectively determine the content of paeoniflorin， albiflorin， benzoylpaeoniflorin and paeonol， which could be used for quality control of Paeoniae Radix Alba., http://www.100md.com(金林 赵万顺 郭巧生 叶正良 张文生)
[关键词]白芍;芍药苷;芍药内酯苷;苯甲酰芍药苷;丹皮酚;UPLC;含量测定;主成分综合评分;质量评价
, http://www.100md.com
[收稿日期]2014-10-15
[通信作者]*郭巧生，Tel：(025)84395980，E-mail：gqs@njau.edu.cn;*叶正良，研究员，Tel：(022)86342066，E-mail：yezl@yahoo.com
[作者简介]金林，硕士研究生，Tel：18105167378，E-mail：2012104202@njau.edu.cnDetermination of chemical components of Paeoniae Radix
Alba decoction pieces and its quality evaluationJIN Lin1， ZHAO Wan-shun2， GUO Qiao-sheng1*， YE Zheng-liang3* ， ZHANG Wen-sheng2
, http://www.100md.com (1. Institute of Chinese Medicinal Materials， Nanjing Agricultural University， Nanjing 210095， China;
2. Tasly Pharmaceutical Group Company Limited， Tianjin 300402， China;
3.Tasly Holding Group Company Limited， Tianjin 300402， China)[Abstract]Objective： An UPLC method was developed to evaluate the quality of Paeoniae Radix by simultaneously determining four components， paeoniflorin， albiflorin， benzoylpaeoniflorin and paeonol in Paeoniae Radix Alba decoction pieces. Method： The UPLC chromatographic column was ACQUITY UPLC HSS T3. The mobile phase was acetonitrile-0.05%phosphoric acid water with gradient elution. The column temperature was 30 ℃ and detection wavelength was 230 nm with a flow rate of 0.4 mL·min-1. A linear model was obtained through principal component analysis (PCA)， and PCA scores were used to evaluate the quality of Radix Paeoniae Alba decoction pieces comprehensively. Result： Paeoniflorin， albiflorin， benzoylpaeoniflorin and paeonol could be well separated from other components， and the results of specificity， precision， repeatability， linearity， recovery rate and stability reached the standards， respectively. The content of paeoniflorin in 9 batches of Paeoniae Radix Alba decoction pieces was below the standard given by Chinese Pharmacopoeia (2010 edition). Using the comprehensive scoring method with principal component analysis， the results showed that the samples from Zhejiang province have better quality than those from Anhui and Shandong provinces. Conclusion： The method established in this study can effectively determine the content of paeoniflorin， albiflorin， benzoylpaeoniflorin and paeonol， which could be used for quality control of Paeoniae Radix Alba., http://www.100md.com(金林 赵万顺 郭巧生 叶正良 张文生)