朱砂安神丸、朱砂、硫化汞、氯化汞、甲基汞对小鼠肾转运体基因表达的影响(1)
[摘要]目的：探讨朱砂安神丸、朱砂、硫化汞(HgS)、氯化汞(HgCl2)和甲基汞(MeHg)对小鼠肾转运体基因表达的影响。方法：将健康雄性小鼠分别ig等量生理盐水、朱砂安神丸1.8 g·kg-1(含汞0.17 g·kg-1)、朱砂0.2 g·kg-1(含汞0.17 g·kg-1)、高剂量朱砂2 g·kg-1(含汞1.7 g·kg-1)、HgS 0.2 g·kg-1(含汞0.17 g·kg-1)、HgCl2 0.032 g·kg-1(含汞0.024 g·kg-1)、MeHg 0.026 g·kg-1(含汞0.024 g·kg-1)，每天1次，连续30 d，记录小鼠体重变化。30 d后处死取肾脏，双道原子荧光光度法检测小鼠肾组织汞蓄积量，RT-PCR方法检测小鼠肾组织有机阴离子转运体基因(Oat1，Oat3，Oat2)、多药耐药相关蛋白基因(Mrp2，Mrp4)、尿酸转运体基因(Urat1)的表达。结果：与正常组相比，HgCl2组和MeHg组小鼠肾汞蓄积量显著升高(P<0.05)，其余各组肾汞蓄积量与正常组无显著差异;HgCl2组和MeHg组Oat1，Oat2表达显著降低(P<0.05);MeHg组Mrp2基因的表达显著升高(P<0.05);HgCl2组和MeHg组Mrp4基因表达显著升高(P<0.05);MeHg组Urat1基因表达显著降低(P<0.05)。结论：HgCl2组和MeHg组小鼠肾汞蓄积量、肾转运体基因表达与正常组有显著差异，其余各组各项检测指标与正常组小鼠相比无显著差异。与HgCl2和MeHg相比，朱砂及其复方对小鼠造成的肾毒性相对较低。
, 百拇医药
[关键词]朱砂;朱砂安神丸;氯化汞;甲基汞;肾转运体
[收稿日期]2014-05-15
[基金项目]贵州省国际合作项目(G2011-7020);贵州省中药现代化项目(2010-5);贵阳中医学院研究生教育创新计划项目(ZYYCX12028);黔科合重大专项字([2010]6019)
[通信作者]*时京珍，Tel：(0851)5622507，E-mail：851839203@qq.com
[作者简介]隋怡，硕士研究生，E-mail：suiyi-de-youxiang@163.comEffect of Zhusha Anshen pill， cinnabar， HgS， HgCl2 and MeHg
on gene expression of renal transporters in miceSUI Yi1， YANG Hong1， TIAN Xing-zhong1， LIU Jie2， SHI Jing-zhen1*
, http://www.100md.com
(1. Experimental Center Basic Medical Laboratory of Experimental Center， Guiyang Traditional Medical College， Guiyang 550002， China;
2. Department of Pharmacology of Zunyi Medical College， Key Laboratory of Basic Pharmacology of Guizhou， Zunyi 563003， China)[Abstract]Objective： To study the effect of Zhusha Anshen pill， cinnabar， HgS， HgCl2 and MeHg on the gene expression of renal transporters in mice. Method： Healthy male mice were given equivalent physiological saline， Zhusha Anshen pill (1.8 g·kg-1， containing 0.17 g·kg-1 of mercury)， cinnabar (0.2 g·kg-1， containing 1.7 g·kg-1 of mercury)， high dose cinnabar (2 g·kg-1， containing 1.7 g·kg-1 of mercury)， HgS (0.2 g·kg-1， containing 0.17 g·kg-1 of mercury)， HgCl2 (0.032 g·kg-1， containing 0.024 g·kg-1 of mercury)， MeHg(0.026 g·kg-1， containing 0.024 g·kg-1 of mercury)， once daily， for 30 d， measuring body mass gain. 30 days later， the mice were sacrificed. The mercury accumulation in kidneys was detected with atomic fluorescence spectrometer. Expressions of Oat1，Oat2， Oat3， Mrp2， Mrp4，Urat1 were detected with RT-PCR. Result： Compared with the normal control group， a significant accumulation of Hg in kidney in HgCl2 and MeHg groups was observed (P<0.05)， but these changes were not found in other groups. Compared with normal control group， mRNA expressions of Oat1 and Oat2 were evidently lower in HgCl2 and MeHg groups， but mRNA expressions of Mrp2 were apparently higher in HgCl2 group (P<0.05)， mRNA expression of Mrp4 was significant higher in HgCl2 and MeHg groups， and mRNA expression of Urat1 was apparently lower in MeHg group. Conclusion： HgCl2 and MeHg groups show significant difference from the normal group in mercury accumulation in kidneys and gene expression of kidney transporters， but with no difference between other groups and the normal group. Compared with HgCl2 and MeHg， cinnabar and its compounds could cause lower renal toxicity to mice., http://www.100md.com(隋怡 杨虹 田兴中 刘杰 时京珍)
, 百拇医药
[关键词]朱砂;朱砂安神丸;氯化汞;甲基汞;肾转运体
[收稿日期]2014-05-15
[基金项目]贵州省国际合作项目(G2011-7020);贵州省中药现代化项目(2010-5);贵阳中医学院研究生教育创新计划项目(ZYYCX12028);黔科合重大专项字([2010]6019)
[通信作者]*时京珍，Tel：(0851)5622507，E-mail：851839203@qq.com
[作者简介]隋怡，硕士研究生，E-mail：suiyi-de-youxiang@163.comEffect of Zhusha Anshen pill， cinnabar， HgS， HgCl2 and MeHg
on gene expression of renal transporters in miceSUI Yi1， YANG Hong1， TIAN Xing-zhong1， LIU Jie2， SHI Jing-zhen1*
, http://www.100md.com
(1. Experimental Center Basic Medical Laboratory of Experimental Center， Guiyang Traditional Medical College， Guiyang 550002， China;
2. Department of Pharmacology of Zunyi Medical College， Key Laboratory of Basic Pharmacology of Guizhou， Zunyi 563003， China)[Abstract]Objective： To study the effect of Zhusha Anshen pill， cinnabar， HgS， HgCl2 and MeHg on the gene expression of renal transporters in mice. Method： Healthy male mice were given equivalent physiological saline， Zhusha Anshen pill (1.8 g·kg-1， containing 0.17 g·kg-1 of mercury)， cinnabar (0.2 g·kg-1， containing 1.7 g·kg-1 of mercury)， high dose cinnabar (2 g·kg-1， containing 1.7 g·kg-1 of mercury)， HgS (0.2 g·kg-1， containing 0.17 g·kg-1 of mercury)， HgCl2 (0.032 g·kg-1， containing 0.024 g·kg-1 of mercury)， MeHg(0.026 g·kg-1， containing 0.024 g·kg-1 of mercury)， once daily， for 30 d， measuring body mass gain. 30 days later， the mice were sacrificed. The mercury accumulation in kidneys was detected with atomic fluorescence spectrometer. Expressions of Oat1，Oat2， Oat3， Mrp2， Mrp4，Urat1 were detected with RT-PCR. Result： Compared with the normal control group， a significant accumulation of Hg in kidney in HgCl2 and MeHg groups was observed (P<0.05)， but these changes were not found in other groups. Compared with normal control group， mRNA expressions of Oat1 and Oat2 were evidently lower in HgCl2 and MeHg groups， but mRNA expressions of Mrp2 were apparently higher in HgCl2 group (P<0.05)， mRNA expression of Mrp4 was significant higher in HgCl2 and MeHg groups， and mRNA expression of Urat1 was apparently lower in MeHg group. Conclusion： HgCl2 and MeHg groups show significant difference from the normal group in mercury accumulation in kidneys and gene expression of kidney transporters， but with no difference between other groups and the normal group. Compared with HgCl2 and MeHg， cinnabar and its compounds could cause lower renal toxicity to mice., http://www.100md.com(隋怡 杨虹 田兴中 刘杰 时京珍)