基于网络药理学的五味子木脂素类主要药效作用研究(1)
[摘要]五味子木脂素是五味子的主要药效物质，该研究对木脂素类6个主要成分五味子甲素、乙素、丙素、醇甲、醇乙和酯甲进行文献检索，收集这些成分作用的基因，并采用网络药理学的方法构建成分-基因-通路网络，经网络分析得到124个基因，共富集到88条通路，其中与炎症相关的通路有32条，共涉及80个基因，提示抗炎可能是6个成分的主要药效作用。随后采用LPS诱导RAW264.7细胞的炎症模型考察发现，6个成分中除五味子醇乙外皆能降低NO含量，表现出抗炎作用，与网络分析结果大致相同。进一步考察五味子甲素、丙素、醇甲和酯甲对炎性因子TNF-α，IL-1β，IL-6，PGE2分泌及iNOS，COX-2蛋白表达的影响，结果显示五味子甲素对4个炎性因子分泌均有抑制作用，并能同时减少iNOS，COX-2蛋白的表达，具有最强的抗炎活性。该研究为系统研究五味子的药效作用及其机制提供了参考。
[关键词]五味子木脂素;网络药理学;炎症;基因;通路
[收稿日期]2014-11-11
, http://www.100md.com
[基金项目]国家自然科学基金项目(81173467)
[通信作者]*赵筱萍，教授，博士生导师，Tel：(0571)86633138， E-mail： zhaoxiaoping@zcmu.edu.cn
[作者简介]邱宏涛，硕士研究生，E-mail：qiuhongtao767@163.comStudy on main pharmacodynamic effects for Schisandra lignans
based upon network pharmacology
QIU Hong-tao1， ZHAO Xiao-ping1*， LI Zheng2， WANG Lin-li3， WANG Yi3
(1. Zhejiang Chinese Medical University， Hangzhou 310053， China;
, 百拇医药
2. State Key Laboratory of Modern Chinese Medicine， Tianjin University of Traditional Chinese Medicine， Tianjin 300193， China;
3. Department of Chinese Medicine Science & Engineering， College of Pharmaceutical Sciences， Zhejiang University， Hangzhou 310058， China)
[Abstract]In this study， we focused on the study of pharmacodynamic effects for 6 major bioactive lignans of Schisandra chinensis， namely deoxyschizandrin， schisandrin B， schisandrin C， schisandrin， schizandrol B and schisantherin A. A compound-gene-pathway network， which contained 124 related genes and 88 pathways， was constructed by collecting drug genes through mining relevant literatures and network pharmacology analysis. Based on the network analysis， 32 pathways and 80 related gene were associated with inflammation， which implied that anti-inflammatory might be the major pharmacodynamic effects of these compounds. All lignans except schizandrol B reduced LPS-induced NO production in RAW264.7 cells， which validated the anti-inflammatory hypothesis generated from network analysis. Furthermore， we investigated the effects of deoxyschizandrin， schisandrin C， schisandrin and schisantherin A on the secretion of inflammatory cytokines TNF-α， IL-1β， IL-6， PGE2 and protein expressions of iNOS， COX-2. As a result， deoxyschizandrin showed the strongest anti-inflammatory activity with inhibitory effect on all 4 inflammatory cytokines secretions and protein expressions of iNOS， COX-2. This study provided evidences for systematic exploration on the pharmacolgical actions and mechanisms of schisandra.
[Key words]Schisandra lignans; network pharmacology; inflammation; gene; pathway, 百拇医药(邱宏涛 赵筱萍 李正 王林丽 王毅)
[关键词]五味子木脂素;网络药理学;炎症;基因;通路
[收稿日期]2014-11-11
, http://www.100md.com
[基金项目]国家自然科学基金项目(81173467)
[通信作者]*赵筱萍，教授，博士生导师，Tel：(0571)86633138， E-mail： zhaoxiaoping@zcmu.edu.cn
[作者简介]邱宏涛，硕士研究生，E-mail：qiuhongtao767@163.comStudy on main pharmacodynamic effects for Schisandra lignans
based upon network pharmacology
QIU Hong-tao1， ZHAO Xiao-ping1*， LI Zheng2， WANG Lin-li3， WANG Yi3
(1. Zhejiang Chinese Medical University， Hangzhou 310053， China;
, 百拇医药
2. State Key Laboratory of Modern Chinese Medicine， Tianjin University of Traditional Chinese Medicine， Tianjin 300193， China;
3. Department of Chinese Medicine Science & Engineering， College of Pharmaceutical Sciences， Zhejiang University， Hangzhou 310058， China)
[Abstract]In this study， we focused on the study of pharmacodynamic effects for 6 major bioactive lignans of Schisandra chinensis， namely deoxyschizandrin， schisandrin B， schisandrin C， schisandrin， schizandrol B and schisantherin A. A compound-gene-pathway network， which contained 124 related genes and 88 pathways， was constructed by collecting drug genes through mining relevant literatures and network pharmacology analysis. Based on the network analysis， 32 pathways and 80 related gene were associated with inflammation， which implied that anti-inflammatory might be the major pharmacodynamic effects of these compounds. All lignans except schizandrol B reduced LPS-induced NO production in RAW264.7 cells， which validated the anti-inflammatory hypothesis generated from network analysis. Furthermore， we investigated the effects of deoxyschizandrin， schisandrin C， schisandrin and schisantherin A on the secretion of inflammatory cytokines TNF-α， IL-1β， IL-6， PGE2 and protein expressions of iNOS， COX-2. As a result， deoxyschizandrin showed the strongest anti-inflammatory activity with inhibitory effect on all 4 inflammatory cytokines secretions and protein expressions of iNOS， COX-2. This study provided evidences for systematic exploration on the pharmacolgical actions and mechanisms of schisandra.
[Key words]Schisandra lignans; network pharmacology; inflammation; gene; pathway, 百拇医药(邱宏涛 赵筱萍 李正 王林丽 王毅)