阿魏酸对巨噬细胞泡沫样化过程中胆固醇流出的影响及可能机制(1)
[摘要]巨噬细胞泡沫样改变是动脉粥样硬化(AS)的特征性变化，胆固醇外流受阻是引起巨噬细胞泡沫样改变的重要环节之一。该文拟采用氧化低密度脂蛋白(ox-LDL)诱导巨噬细胞源性泡沫细胞的形成，然后用不同浓度的阿魏酸对巨噬细胞源性泡沫细胞进行处理，观察阿魏酸对ox-LDL诱导巨噬细胞泡沫样化过程中细胞内脂质代谢、胆固醇流出及介导胆固醇流出的ATP结合盒转运蛋白A1(ABCA1)和ATP结合盒转运蛋白G1(ABCG1)的mRNA和蛋白水平的影响，探讨阿魏酸抗AS的可能机制。结果显示，与对照组相比，ox-LDL 处理组细胞内脂质含量明显增加，主要表现为胆固醇酯的含量增加;用阿魏酸处理泡沫样细胞后，细胞内脂质聚集明显减少，尤其是胆固醇酯的含量明显降低，同时胆固醇的流出也显著增加;研究还发现用不同浓度阿魏酸处理泡沫样细胞后，细胞表面ABCA1和ABCG1的mRNA和蛋白表达水平均升高。综上所述，阿魏酸可能通过增加巨噬细胞源性泡沫细胞表面ABCA1和ABCG1的表达水平，促进胆固醇流出，从而发挥抗AS的作用。
[关键词]阿魏酸;动脉粥样硬化;胆固醇流出;ATP结合盒转运蛋白
, 百拇医药
[收稿日期]2014-05-30
[通信作者]*陈阜新，副主任药师，研究方向为中药药理学，Tel：13907119777， E-mail：whcoltene@sohu.comEffect of ferulic acid on cholesterol efflux in macrophage foam
cell formation and potential mechanismCHEN Fu-xin1*， WANG Lian-kai2
(1.Wuhan University， Wuhan 430072， China; 2. Hubei Institute for Nationalities Major Hospital Affiliated China， Enshi 445000， China)[Abstract]The formation of macrophage-derived foam cells is a typical feature of atherosclerosis (AS). Reverse cholesterol efflux (RCT) is one of important factors for the formation of macrophage foam cells. In this study， macrophage form cells were induced by oxidized low density lipoprotein (ox-LDL) and then treated with different concentrations of ferulic acid， so as to observe the effect of ferulic acid on the intracellular lipid metabolism in the ox-LDL-induced macrophage foam cell formation， the cholesterol efflux and the mRNA expression and protein levels of ATP binding cassette transporter A1 (ABCA1) and ATP binding cassette transporter G1 (ABCG1) that mediate cholesterol efflux， and discuss the potential mechanism of ferulic acid in resisting AS. According to the findings， compared with the control group， the ox-LDL-treated group showed significant increase in intracellular lipid content， especially for the cholesterol content; whereas the intracellular lipid accumulation markedly decreased， after the treatment with ferulic acid. The data also demonstrated that the mRNA and protein expressions of ABCA1 and ABCG1 significantly increased after macrophage foam cells were treated with different concentrations of ferulic acid. In summary， ferulic acid may show the anti-atherosclerosis effect by increasing the surface ABCA1 and ABCG1expressions of macrophage form cells and promoting cholesterol efflux.
[Key words]ferulic acid; atherosclerosis; cholesterol efflux; ATP binding cassette transporter
doi：10.4268/cjcmm20150330
动脉粥样硬化(atherosclerosis， AS)是导致冠状动脉疾病发病和死亡的主要原因， 而巨噬细胞泡沫样改变是AS的特征性病理改变。泡沫样细胞的形成与细胞内脂质代谢异常密切相关，胆固醇摄入过多或者外流减少，均可引起脂质在细胞内的聚集而使巨噬细胞转化为泡沫样细胞[1-3]。, 百拇医药(陈阜新 汪莲开)
[关键词]阿魏酸;动脉粥样硬化;胆固醇流出;ATP结合盒转运蛋白
, 百拇医药
[收稿日期]2014-05-30
[通信作者]*陈阜新，副主任药师，研究方向为中药药理学，Tel：13907119777， E-mail：whcoltene@sohu.comEffect of ferulic acid on cholesterol efflux in macrophage foam
cell formation and potential mechanismCHEN Fu-xin1*， WANG Lian-kai2
(1.Wuhan University， Wuhan 430072， China; 2. Hubei Institute for Nationalities Major Hospital Affiliated China， Enshi 445000， China)[Abstract]The formation of macrophage-derived foam cells is a typical feature of atherosclerosis (AS). Reverse cholesterol efflux (RCT) is one of important factors for the formation of macrophage foam cells. In this study， macrophage form cells were induced by oxidized low density lipoprotein (ox-LDL) and then treated with different concentrations of ferulic acid， so as to observe the effect of ferulic acid on the intracellular lipid metabolism in the ox-LDL-induced macrophage foam cell formation， the cholesterol efflux and the mRNA expression and protein levels of ATP binding cassette transporter A1 (ABCA1) and ATP binding cassette transporter G1 (ABCG1) that mediate cholesterol efflux， and discuss the potential mechanism of ferulic acid in resisting AS. According to the findings， compared with the control group， the ox-LDL-treated group showed significant increase in intracellular lipid content， especially for the cholesterol content; whereas the intracellular lipid accumulation markedly decreased， after the treatment with ferulic acid. The data also demonstrated that the mRNA and protein expressions of ABCA1 and ABCG1 significantly increased after macrophage foam cells were treated with different concentrations of ferulic acid. In summary， ferulic acid may show the anti-atherosclerosis effect by increasing the surface ABCA1 and ABCG1expressions of macrophage form cells and promoting cholesterol efflux.
[Key words]ferulic acid; atherosclerosis; cholesterol efflux; ATP binding cassette transporter
doi：10.4268/cjcmm20150330
动脉粥样硬化(atherosclerosis， AS)是导致冠状动脉疾病发病和死亡的主要原因， 而巨噬细胞泡沫样改变是AS的特征性病理改变。泡沫样细胞的形成与细胞内脂质代谢异常密切相关，胆固醇摄入过多或者外流减少，均可引起脂质在细胞内的聚集而使巨噬细胞转化为泡沫样细胞[1-3]。, 百拇医药(陈阜新 汪莲开)