不同气候区丹参生物量、有效成分变化与气象因子的相关性研究(1)
[摘要]通过在陕西境内由南向北的3种典型气候区以及过渡带布设22个试验区，将同一种源丹参种苗于春季栽植于上述试验区，统一规范人工栽培管理，采用HPLC检测样品的二萜醌类成分(二氢丹参酮、隐丹参酮、丹参酮Ⅰ和丹参酮ⅡA)，并测定生物量数据(根长、分根数、根直径和根干重)。由中国气象科学数据共享中心平台和陕西省农业气象服务中心获取试验区的降雨量、年均温、昼夜温差等10个关键农业生态气象因子。试验结果表明：同一种源的丹参在不同气候生境条件下获得的所有试验区丹参样品经检测均符合国家药典的限量标准，二氢丹参酮含量的变异系数最大。不同气候区的丹参根系形态差别较大。相关性分析结果表明：4—10月相对湿度、4—10月水气压、4—10月累积日照时数、4—10月昼夜温差和年均温是影响丹参品质的主要限制性气象因子，而4—10月20 cm平均地温、年活动积温、年均温、4—10月水气压、4—10月昼夜温差是影响丹参产量的主要限制性气象因子。丹参酮类物质均随纬度降低而升高，根的干物质积累量基本随纬度升高而降低，且随海拔升高而降低。相对于水分和温度条件，丹参对日照时数长短的要求并不严格。丹参可以适应多种气候条件，完成其正常生命过程，但与陕西北部的半干旱季风气候、中部的暖温带半湿润气候相比，南部的北亚热带湿润季风气候的相对温暖湿润气候条件更利于丹参的生长和有效成分的累积。
, http://www.100md.com
[关键词]生态环境;气象因子;丹参;丹参酮;次生代谢;产量;品质
Correlation analysis between meteorological factors， biomass， and active
components of Salvia miltiorrhiza in different climatic zones
ZHANG Chen-lu1，2， LIANG Zong-suo1，3*， GUO Hong-bo1， LIU Jing-ling1， LIU Yan4， LIU Feng-hua4， WEI Liang-zhu4
(1. Northwest Agriculture and Forestry University of Science and Technology， College of Life Science， Yangling 712100 China;
, 百拇医药
2. Shaanxi University of Technology，College of Biological Science & Engineering， Hanzhong 723000， China;
3. Zhejiang Science Technology University， College of Life Science， Hangzhou 310018， China;
4. Tianjin Tasly Modern Traditional Chinese Medicine Resources Co.， Ltd.， Tianjin 300410， China)
[Abstract]In this study， the growth and accumulation of active components of Salvia miltiorrhiza in twenty two experimental sites which crossing through three typical climate zones. The S. miltiorrhiza seedlings with the same genotype were planted in each site in spring， which were cultivated in fields with uniform management during their growing seasons till to harvest. The diterpene ketones (dihydrotanshinone， cryptotanshinone， tanshinoneⅠ and tanshinone ⅡA) in S. miltiorrhiza root samples were determined by using high-performance liquid chromatography (HPLC) method. The biomass of root (root length， number of root branches， root width and dry weight) was also measured. The results showed that tanshinone ⅡA in all samples of each site were higher than the standards required by China Pharmacopoeia. It has been found there is a relationship between root shape and climate change. The correlation analysis between active components and meteorological factors showed that the accumulation of tanshinones were effected by such meteorological factors as average relative humidity from April to October> average vapor pressure from April to October > average temperature difference day and night from April to October > annual average temperature and so on. The correlation analysis between root biomass and meteorological factors exhibited that root shape and accumulation of dry matter were affected by those factors， such as average annual aboveground (0-20 cm) temperature from April to October > annual average temperature > average vapor pressure from April to October > annual active accumulated temperature> annual average temperature > average vapor pressure from April to October. The accumulation of tanshinones and biomass was increased with the decrease of latitude. At the same time， the dry matter and diameter of root decreased if altitude rises. In addition， S. miltiorrhiza required sunlight is not sophisticated， when compared with humid and temperature. To sum up， S. miltiorrhiza can adapt to a variety of climatic conditions and the southern warm humid climate is more conducive to its growth and accumulation of active components., http://www.100md.com(张辰露 梁宗锁 郭宏波 刘景玲 刘岩 刘峰华 魏良柱)
, http://www.100md.com
[关键词]生态环境;气象因子;丹参;丹参酮;次生代谢;产量;品质
Correlation analysis between meteorological factors， biomass， and active
components of Salvia miltiorrhiza in different climatic zones
ZHANG Chen-lu1，2， LIANG Zong-suo1，3*， GUO Hong-bo1， LIU Jing-ling1， LIU Yan4， LIU Feng-hua4， WEI Liang-zhu4
(1. Northwest Agriculture and Forestry University of Science and Technology， College of Life Science， Yangling 712100 China;
, 百拇医药
2. Shaanxi University of Technology，College of Biological Science & Engineering， Hanzhong 723000， China;
3. Zhejiang Science Technology University， College of Life Science， Hangzhou 310018， China;
4. Tianjin Tasly Modern Traditional Chinese Medicine Resources Co.， Ltd.， Tianjin 300410， China)
[Abstract]In this study， the growth and accumulation of active components of Salvia miltiorrhiza in twenty two experimental sites which crossing through three typical climate zones. The S. miltiorrhiza seedlings with the same genotype were planted in each site in spring， which were cultivated in fields with uniform management during their growing seasons till to harvest. The diterpene ketones (dihydrotanshinone， cryptotanshinone， tanshinoneⅠ and tanshinone ⅡA) in S. miltiorrhiza root samples were determined by using high-performance liquid chromatography (HPLC) method. The biomass of root (root length， number of root branches， root width and dry weight) was also measured. The results showed that tanshinone ⅡA in all samples of each site were higher than the standards required by China Pharmacopoeia. It has been found there is a relationship between root shape and climate change. The correlation analysis between active components and meteorological factors showed that the accumulation of tanshinones were effected by such meteorological factors as average relative humidity from April to October> average vapor pressure from April to October > average temperature difference day and night from April to October > annual average temperature and so on. The correlation analysis between root biomass and meteorological factors exhibited that root shape and accumulation of dry matter were affected by those factors， such as average annual aboveground (0-20 cm) temperature from April to October > annual average temperature > average vapor pressure from April to October > annual active accumulated temperature> annual average temperature > average vapor pressure from April to October. The accumulation of tanshinones and biomass was increased with the decrease of latitude. At the same time， the dry matter and diameter of root decreased if altitude rises. In addition， S. miltiorrhiza required sunlight is not sophisticated， when compared with humid and temperature. To sum up， S. miltiorrhiza can adapt to a variety of climatic conditions and the southern warm humid climate is more conducive to its growth and accumulation of active components., http://www.100md.com(张辰露 梁宗锁 郭宏波 刘景玲 刘岩 刘峰华 魏良柱)