四逆汤配伍环境下的附子“效—毒网络”交集调控研究(1)
[摘要]目的：采用网络药理学方法探索四逆汤配伍环境下，甘草、干姜对附子作用基因“效-毒网络”交集调控的机制。方法：应用Cytoscape软件和插件Agilent Literature Search，进行文本挖掘并建立附子、甘草、干姜作用的基因相互关系网络;根据附子抗心衰、神经毒性和心脏毒性形成附子“效-毒网络”交集;应用插件Clusterviz进行靶标基因聚类，从DAVID数据库中对甘草、干姜调控附子“效-毒网络”交集的可能通路进行预测。结果：与附子神经毒性、心脏毒性和抗心衰作用同时相关的基因有5个，AKT1，BAX，HCC，IL6，IL8，形成附子“效-毒网络”交集有47个节点基因;甘草、干姜与附子“效-毒网络”交集的重合基因分别有29个和27个，可能调控通路有23条和17条。结论：在四逆汤配伍环境下，甘草、干姜可能通过影响免疫-炎症反应信号通路、细胞凋亡-自噬信号通路、神经细胞与心肌细胞缺血缺氧保护信号通路等途径调控附子的效/毒效应。
[关键词]四逆汤;效-毒网络;网络药理学
, 百拇医药
Study on intersection and regulation mechanism of ″efficacy-toxicity
network″ of aconite in combination environment of Sini decoction
LI Zhi-yong1， BAO Hong-juan2， ZHANG Shuo-feng3， YE Tian-yuan1， YANG Ce4， LI Yan-wen4*
(1. China Minority Traditional Medical Center， Minzu University of China， Beijing 100081， China; 2. Xiamen Medical College，Xiamen 361008， China; 3.School of Chinese Materia Medica of Beijing University of Chinese Medicine， Beijing 100102， China;
, http://www.100md.com
4. Institute of Information on Traditional Chinese Medicine， China Academy of Chinese Medical Sciences， Beijing 100700， China)
[Abstract]Objective： To explore the intersection and regulation mechanism of ″efficacy-toxicity network″ of Glycyrrhizae Radix et Rhizoma， Zingiberis Rhizoma and Aconiti Lateralis Radix Praeparata′s action gene in the combination environment of Sini decoction with the network pharmacological method. Method： The gene interaction network of Aconiti Lateralis Radix Praeparata， Glycyrrhizae Radix et Rhizoma， Zingiberis Rhizoma were mined and established with Cytoscape software and Agilent literature search plug-in. The ″efficiency-toxicity network″ intersection of Aconiti Lateralis Radix Praeparata was formed according to its effects in anti-heart failure， neurotoxicity and cardiotoxicity. The target genes were clustered with Clusterviz plug-in. And the possible pathways of the ″efficacy-toxicity network″ intersection of Glycyrrhizae Radix et Rhizoma， Zingiberis Rhizoma and Aconiti Lateralis Radix Praeparata were forecasted in DAVID database. Result： There were five genes related to neurotoxicity， cardiotoxicity and anti-heart failure function of Aconiti Lateralis Radix Praeparata， namely AKT1， BAX， HCC， IL6 and IL8， which formed 47 nodes genes in the ″efficiency-toxicity network″ intersection of Aconiti Lateralis Radix Praeparata. There were 29 and 27 coincident genes in the ″efficiency-toxicity network″ of Glycyrrhizae Radix et Rhizoma， Zingiberis Rhizoma and Aconiti Lateralis Radix Praeparata. There were 23 and 17 possible regulatory pathways. Conclusion： In the combination environment of Sini decoction， Glycyrrhizae Radix et Rhizoma and Zingiberis Rhizoma may regulate the efficiency-toxicity network of Aconiti Lateralis Radix Praeparata by influencing immune-inflammatory signaling pathway， apoptosis-autophagy signaling pathway， nerve cell and myocardial ischemia and hypoxia protection signaling pathways., http://www.100md.com(李志勇 鲍红娟 张硕峰 叶田园 杨策 李彦文)
[关键词]四逆汤;效-毒网络;网络药理学
, 百拇医药
Study on intersection and regulation mechanism of ″efficacy-toxicity
network″ of aconite in combination environment of Sini decoction
LI Zhi-yong1， BAO Hong-juan2， ZHANG Shuo-feng3， YE Tian-yuan1， YANG Ce4， LI Yan-wen4*
(1. China Minority Traditional Medical Center， Minzu University of China， Beijing 100081， China; 2. Xiamen Medical College，Xiamen 361008， China; 3.School of Chinese Materia Medica of Beijing University of Chinese Medicine， Beijing 100102， China;
, http://www.100md.com
4. Institute of Information on Traditional Chinese Medicine， China Academy of Chinese Medical Sciences， Beijing 100700， China)
[Abstract]Objective： To explore the intersection and regulation mechanism of ″efficacy-toxicity network″ of Glycyrrhizae Radix et Rhizoma， Zingiberis Rhizoma and Aconiti Lateralis Radix Praeparata′s action gene in the combination environment of Sini decoction with the network pharmacological method. Method： The gene interaction network of Aconiti Lateralis Radix Praeparata， Glycyrrhizae Radix et Rhizoma， Zingiberis Rhizoma were mined and established with Cytoscape software and Agilent literature search plug-in. The ″efficiency-toxicity network″ intersection of Aconiti Lateralis Radix Praeparata was formed according to its effects in anti-heart failure， neurotoxicity and cardiotoxicity. The target genes were clustered with Clusterviz plug-in. And the possible pathways of the ″efficacy-toxicity network″ intersection of Glycyrrhizae Radix et Rhizoma， Zingiberis Rhizoma and Aconiti Lateralis Radix Praeparata were forecasted in DAVID database. Result： There were five genes related to neurotoxicity， cardiotoxicity and anti-heart failure function of Aconiti Lateralis Radix Praeparata， namely AKT1， BAX， HCC， IL6 and IL8， which formed 47 nodes genes in the ″efficiency-toxicity network″ intersection of Aconiti Lateralis Radix Praeparata. There were 29 and 27 coincident genes in the ″efficiency-toxicity network″ of Glycyrrhizae Radix et Rhizoma， Zingiberis Rhizoma and Aconiti Lateralis Radix Praeparata. There were 23 and 17 possible regulatory pathways. Conclusion： In the combination environment of Sini decoction， Glycyrrhizae Radix et Rhizoma and Zingiberis Rhizoma may regulate the efficiency-toxicity network of Aconiti Lateralis Radix Praeparata by influencing immune-inflammatory signaling pathway， apoptosis-autophagy signaling pathway， nerve cell and myocardial ischemia and hypoxia protection signaling pathways., http://www.100md.com(李志勇 鲍红娟 张硕峰 叶田园 杨策 李彦文)