枇杷不同器官及不同物候4种三萜酸含量的动态变化(1)
[摘要] 为考察4种三萜酸成分在枇杷各器官中分布，揭示不同物候枇杷叶三萜酸动态变化规律，观测枇杷物候，采集花、果实以及不同物候期的叶;采用高效液相色谱法测定熊果酸、齐墩果酸、科罗索酸和山楂酸含量。结果表明，4种三萜酸在所测样品器官中的质量分数高低顺序为落叶(23.2 mg·g-1)>成熟叶(21.7 mg·g-1)>嫩叶(17.5 mg·g-1)>果肉(7.36 mg·g-1)>花 (6.40 mg·g-1)，种子中未检出这4种三萜酸成分;在枇杷树萌芽、现蕾、开花、结果等不同物候采收的枇杷叶中4种三萜酸总量发生小幅波动，落叶变化范围17.8～26.2 mg·g-1，成熟叶变化范围在16.5～23.5 mg·g-1，嫩叶变化范围在14.7～21.5 mg·g-1。随叶片发育、成熟、衰老，三萜酸含量有所增加;不同物候阶段对枇杷叶干物质积累及4种三萜酸含量产生一定影响。枇杷成熟叶在果实膨大时的干物质和三萜酸积累较多。文章对地域分布广泛的枇杷等植物药在次生代谢产物和采收期等相关研究上具有一定参考作用。
, 百拇医药
[关键词] 枇杷;物候;不同器官;三萜酸;动态变化
[收稿日期] 2014-09-04
[基金项目] 安徽高校省级自然科学项目(KJ2012A188);安徽中医药大学大学生“药苑之星”科学研究项目(20120306)
[通信作者] 谢晓梅，教授，主要从事中药质量研究，Tel：(0551) 68129153，E-mail：xiexiaomei9401@sina.com
[作者简介] 李继杨，Tel：15956955815，E-mail：949322977@qq.com
Dynamic change of four triterpenic acids contents in different
, http://www.100md.com
organs of loquat (Eriobotrya japonica) and phenology
LI Ji-yang， XIE Xiao-mei， LI Qian-wen， ZHANG Qi， CHEN Sheng-lin，WANG He-qun， YU Wen-xia， YANG Mo
(School of Pharmacy， Anhui University of Chinese Medicine， Hefei 230012， China)
[Abstract] The loquat is widely cultivated in China， its succulent fruits， leaves and flower are used as a traditional medicine for the treatment of many diseases. The study is aimed to analyse the content of the four triterpene compounds ( ursolic acid， corosolic acid， maslinic acid， oleanolic acid) in different organs， and investigate the dynamic changes in different phenological period. The triterpenic acids content in the samples was measured by HPLC based on the plant phenological observations. The results showed that order of four triterpenic acids content in different organs from high to low was defoliation (23.2 mg·g-1)>mature leaves (21.7 mg·g-1)>young leaves (17.5 mg·g-1)>fruits (7.36 mg·g-1) >flowers (6.40 mg·g-1). The triterpenic acids were not detected in the seeds. The total amount of the four triterpenic acids in the loquat leaves collected in the different phenological stages of sprout， flower bud， blossom and fruit varied between 17.8 and 26.2 mg·g-1(defoliation)， 16.5 and 23.5 mg·g-1(mature leaves)， 14.7 and 21.5 mg·g-1(young leaves)， respectively. The content increased progressively with the leaf development， maturation and aging. There was a higher level of the dry material and triterpenic acids accumulation in the mature leaves during fruit enlargement. This paper attempts to present the case for medicinal plants of a broad geographical distribution to study on the secondary metabolites and harvesting time., http://www.100md.com(李继杨 谢晓梅 李倩文 张齐 陈胜林 汪鹤群 余文霞 杨沫)
, 百拇医药
[关键词] 枇杷;物候;不同器官;三萜酸;动态变化
[收稿日期] 2014-09-04
[基金项目] 安徽高校省级自然科学项目(KJ2012A188);安徽中医药大学大学生“药苑之星”科学研究项目(20120306)
[通信作者] 谢晓梅，教授，主要从事中药质量研究，Tel：(0551) 68129153，E-mail：xiexiaomei9401@sina.com
[作者简介] 李继杨，Tel：15956955815，E-mail：949322977@qq.com
Dynamic change of four triterpenic acids contents in different
, http://www.100md.com
organs of loquat (Eriobotrya japonica) and phenology
LI Ji-yang， XIE Xiao-mei， LI Qian-wen， ZHANG Qi， CHEN Sheng-lin，WANG He-qun， YU Wen-xia， YANG Mo
(School of Pharmacy， Anhui University of Chinese Medicine， Hefei 230012， China)
[Abstract] The loquat is widely cultivated in China， its succulent fruits， leaves and flower are used as a traditional medicine for the treatment of many diseases. The study is aimed to analyse the content of the four triterpene compounds ( ursolic acid， corosolic acid， maslinic acid， oleanolic acid) in different organs， and investigate the dynamic changes in different phenological period. The triterpenic acids content in the samples was measured by HPLC based on the plant phenological observations. The results showed that order of four triterpenic acids content in different organs from high to low was defoliation (23.2 mg·g-1)>mature leaves (21.7 mg·g-1)>young leaves (17.5 mg·g-1)>fruits (7.36 mg·g-1) >flowers (6.40 mg·g-1). The triterpenic acids were not detected in the seeds. The total amount of the four triterpenic acids in the loquat leaves collected in the different phenological stages of sprout， flower bud， blossom and fruit varied between 17.8 and 26.2 mg·g-1(defoliation)， 16.5 and 23.5 mg·g-1(mature leaves)， 14.7 and 21.5 mg·g-1(young leaves)， respectively. The content increased progressively with the leaf development， maturation and aging. There was a higher level of the dry material and triterpenic acids accumulation in the mature leaves during fruit enlargement. This paper attempts to present the case for medicinal plants of a broad geographical distribution to study on the secondary metabolites and harvesting time., http://www.100md.com(李继杨 谢晓梅 李倩文 张齐 陈胜林 汪鹤群 余文霞 杨沫)