白芷中3个主要活性成分含量测定及其质量评价研究(1)
[摘要] 采用高效液相色谱(HPLC)的方法，以Platisil ODS C18色谱柱(4.6 mm×250 mm，5 μm)，流动相乙腈-水，流速1.0 mL·min-1，柱温30 ℃，检测波长254 nm的色谱条件下同时测定白芷药材中的白当归素、欧前胡素和异欧前胡素等3个指标成分的含量。结果显示白当归素、欧前胡素和异欧前胡素的分离度良好，在选定的浓度范围内线性关系良好(R2≥0.999 8)，平均加样回收率分别为100.83%，100.10%，103.52%，RSD分别为1.7%，0.77%，0.41%(n= 6)。表明所建立的方法重复性、耐用性良好，并对不同批次不同产地的白芷药材进行分析，结果显示产自安徽亳州和河南长葛的白芷中所测定的3个成分高于其他产地，为白芷药材的质量评价和控制提供科学依据。
[关键词] 白芷;高效液相色谱;含量测定
[收稿日期] 2014-10-30
, http://www.100md.com
[基金项目] 国家自然科学基金青年项目(81202793);国家自然科学基金面上项目(81473414)
[通信作者] 刘峰，主任药师，博士研究生，主要从事中药新药研究与开发，Tel：(029)88318318，E-mail：liu1720@163.com;杨洪军，博士，研究员，主要从事中药新药设计研究，Tel：(010)64014411-2948，E-mail：hongjun0420@vip.sina.com
Quantitative determination of three active ingredients for
quality evaluation of Angelica dahurica
SHI Yang， LEI Yun， XU Hai-yu， ZHANG Yi， LI De-feng，LIU Feng， YANG Hong-jun
, http://www.100md.com
(1. Shaanxi University of Chinese Medicine， Xi′an 712046， China;
2. Institute of Chinese Materia Medica， China Academy of Chinese Medical Sciences， Beijing 100700， China;
3.Shaanxi Buchang Pharmaceutical Co.， Ltd.， Xi′an 712000， China;
4. Shaanxi Institude of International Trade & Commerce， Xianyang 712046， China)
[Abstract] A HPLC method has been developed in the current investigation for simultaneous determination of three chemical markers of by akangelicin，imperatorin and isoimperatorin in Radix Angelicae Dahuricae. Separation was performed at 30 ℃ on achromatographic column of Platisil ODS C18(4.6 mm×250 mm，5 μm).The mobile phase was acetonitrile-water.The flow rate was 1.0 mL·min-1 and the detection wavelength was 254 nm.The results showed that the three chemical markers could be well resolved and that in the selected linear range， all calibration curves of the three chemical markers showed good linearity (R2≥0.999 8). The recoveries of byakangelicin，imperatorin and isoimperatorin were 100.83%，100.10% and 103.52%， respectively，and RSD were 1.7%， 0.77% and 0.41%(n=6)， respectively. The data suggested that the developed HPLC method had good reproducibility，robustness，and accuracy， which was suitable for the quality control of Radix Angelicae Dahuricae. Applications of this method showed that the three chemical markers had higher contents in the Bozhou Anhui and Changge Henan than others.
[Key words] Angelhca dahurica; HPLC; quantitative determination, http://www.100md.com(史洋 雷云 许海玉 张毅 李德凤 刘峰 杨洪军)
[关键词] 白芷;高效液相色谱;含量测定
[收稿日期] 2014-10-30
, http://www.100md.com
[基金项目] 国家自然科学基金青年项目(81202793);国家自然科学基金面上项目(81473414)
[通信作者] 刘峰，主任药师，博士研究生，主要从事中药新药研究与开发，Tel：(029)88318318，E-mail：liu1720@163.com;杨洪军，博士，研究员，主要从事中药新药设计研究，Tel：(010)64014411-2948，E-mail：hongjun0420@vip.sina.com
Quantitative determination of three active ingredients for
quality evaluation of Angelica dahurica
SHI Yang， LEI Yun， XU Hai-yu， ZHANG Yi， LI De-feng，LIU Feng， YANG Hong-jun
, http://www.100md.com
(1. Shaanxi University of Chinese Medicine， Xi′an 712046， China;
2. Institute of Chinese Materia Medica， China Academy of Chinese Medical Sciences， Beijing 100700， China;
3.Shaanxi Buchang Pharmaceutical Co.， Ltd.， Xi′an 712000， China;
4. Shaanxi Institude of International Trade & Commerce， Xianyang 712046， China)
[Abstract] A HPLC method has been developed in the current investigation for simultaneous determination of three chemical markers of by akangelicin，imperatorin and isoimperatorin in Radix Angelicae Dahuricae. Separation was performed at 30 ℃ on achromatographic column of Platisil ODS C18(4.6 mm×250 mm，5 μm).The mobile phase was acetonitrile-water.The flow rate was 1.0 mL·min-1 and the detection wavelength was 254 nm.The results showed that the three chemical markers could be well resolved and that in the selected linear range， all calibration curves of the three chemical markers showed good linearity (R2≥0.999 8). The recoveries of byakangelicin，imperatorin and isoimperatorin were 100.83%，100.10% and 103.52%， respectively，and RSD were 1.7%， 0.77% and 0.41%(n=6)， respectively. The data suggested that the developed HPLC method had good reproducibility，robustness，and accuracy， which was suitable for the quality control of Radix Angelicae Dahuricae. Applications of this method showed that the three chemical markers had higher contents in the Bozhou Anhui and Changge Henan than others.
[Key words] Angelhca dahurica; HPLC; quantitative determination, http://www.100md.com(史洋 雷云 许海玉 张毅 李德凤 刘峰 杨洪军)