紫草素对IL—17诱导角质形成细胞增殖及趋化因子表达的影响(1)
[摘要] 目的：观察紫草素(shikonin)对IL-17诱导人角质形成细胞增殖及其分泌趋化因子的影响，探讨紫草素治疗银屑病的作用机制。方法：采用IL-17A(200 μg·L-1)刺激体外培养的HaCaT 细胞，同时加入不同浓度紫草素(2，1mg·L-1)共同作用24 h。CCK-8法测定细胞增殖;ELISA法测定细胞分泌炎症因子IL-23;Real-time PCR法测定细胞内趋化因子CXCL1，CXCL2和CCL20以及β-防御素4(β-defensin4，DEFB4)的表达。结果：紫草素2，1 mg·L-1能显著抑制IL-17A诱导的HaCaT细胞增殖，具有统计学意义(P<0.01);紫草素各浓度组均能减少IL-23分泌，并对细胞内CXCL1，CXCL2，CCL20和DEFB4的表达有一定的抑制作用。结论：紫草素能抑制IL-17A诱导的HaCaT细胞增殖和相关细胞因子的分泌，并可以通过抑制趋化因子募集白细胞，从而达到对银屑病的治疗作用。
[关键词] 紫草素;IL-17;HaCaT细胞;趋化因子;银屑病
, http://www.100md.com
[收稿日期] 2014-08-11
[基金项目] 国家自然科学基金项目(81072810);北京市中医管理局青年基金(QN2012-10)
[通信作者] 李萍，研究员，主要从事中药治疗皮外科疾病的研究，Tel： (010)52176679，E-mail：liping411@yahoo.com.cn
[作者简介] 解欣然，助理研究员，E-mail：xiexinran@sohu.com
Effect of shikonin on proliferation of keratinocytes induced by
interleukin-17 and expression of chemokines
, 百拇医药
XIE Xin-ran， ZHANG Lei， LIU Xin， LIN Yan， ZHANG Lu， LI Ping
(Beijing Institute of Chinese Medicine， Capital Medical University， Beijing Hospital of Traditional
Chinese Medicine， Beijing 100010， China)
[Abstract] Objective： To observe the effect of shikonin on the proliferation of human keratinocytes induced by IL-17 and secretion of chemokines， in order to discuss the mechanism of Shikonin in the treatment of psoriasis. Method： In vitro cultured HaCaT cells were stimulated by IL-17A (200 μg·L-1) and mixed with different concentrations (2， 1 mg·L-1) of shikonin for 24 hours. The cell proliferation was detected by CCK-8 assay. Cell secretion inflammatory factor interleukin-23 (IL-23) was detected by ELISA. The expressions of intracellular chemokines CXCL1， CXCL2， CCL20 and β-defensin 4 (DEFB4) were detected by Real-time PCR. Result： Shikonin (2，1 mg·L-1) could distinctly inhibit HaCaT cell proliferation induced by IL-17A， with statistical difference (P<0.01). Each shikonin group showed decreases in the secretion of IL-23 and inhibition in expressions of intracellular CXCL1， CXCL2， CCL20 and DEFB4. Conclusion： Shikonin could inhibit HaCaT cells proliferation induced by IL-17 and secretion of relevant cytokines and recruit leukocytes by inhibiting chemokines， so as to show the effect in treating psoriasis.
, 百拇医药
[Key words] shikonin; IL-17A; HaCaT cell; chemokine; psoriasis
doi：10.4268/cjcmm20150530
银屑病(psoriasis)是一种免疫性皮肤病，以表皮过度增殖、慢性炎症和T淋巴细胞浸润为主要特征。目前，IL-23/Th17轴被认为是银屑病发生的重要环节。Th17细胞分泌的主要效应细胞因子即IL-17，能够募集中性粒细胞和参与炎症反应，在银屑病患者皮损呈阳性表达[1-3]。紫草素是清热凉血中药紫草的主要有效成分，紫草是治疗银屑病的常用中药之一。已有文献报道紫草素抑制角质形成细胞增殖的作用[4-5]。本研究通过建立IL-17A诱导HaCaT细胞增殖模型，观察紫草素对细胞增殖和分泌趋化因子的影响，尝试从炎症方面探讨紫草素治疗银屑病的可能作用机制。, 百拇医药(解欣然 张蕾 刘欣 林燕 张璐 李萍)
[关键词] 紫草素;IL-17;HaCaT细胞;趋化因子;银屑病
, http://www.100md.com
[收稿日期] 2014-08-11
[基金项目] 国家自然科学基金项目(81072810);北京市中医管理局青年基金(QN2012-10)
[通信作者] 李萍，研究员，主要从事中药治疗皮外科疾病的研究，Tel： (010)52176679，E-mail：liping411@yahoo.com.cn
[作者简介] 解欣然，助理研究员，E-mail：xiexinran@sohu.com
Effect of shikonin on proliferation of keratinocytes induced by
interleukin-17 and expression of chemokines
, 百拇医药
XIE Xin-ran， ZHANG Lei， LIU Xin， LIN Yan， ZHANG Lu， LI Ping
(Beijing Institute of Chinese Medicine， Capital Medical University， Beijing Hospital of Traditional
Chinese Medicine， Beijing 100010， China)
[Abstract] Objective： To observe the effect of shikonin on the proliferation of human keratinocytes induced by IL-17 and secretion of chemokines， in order to discuss the mechanism of Shikonin in the treatment of psoriasis. Method： In vitro cultured HaCaT cells were stimulated by IL-17A (200 μg·L-1) and mixed with different concentrations (2， 1 mg·L-1) of shikonin for 24 hours. The cell proliferation was detected by CCK-8 assay. Cell secretion inflammatory factor interleukin-23 (IL-23) was detected by ELISA. The expressions of intracellular chemokines CXCL1， CXCL2， CCL20 and β-defensin 4 (DEFB4) were detected by Real-time PCR. Result： Shikonin (2，1 mg·L-1) could distinctly inhibit HaCaT cell proliferation induced by IL-17A， with statistical difference (P<0.01). Each shikonin group showed decreases in the secretion of IL-23 and inhibition in expressions of intracellular CXCL1， CXCL2， CCL20 and DEFB4. Conclusion： Shikonin could inhibit HaCaT cells proliferation induced by IL-17 and secretion of relevant cytokines and recruit leukocytes by inhibiting chemokines， so as to show the effect in treating psoriasis.
, 百拇医药
[Key words] shikonin; IL-17A; HaCaT cell; chemokine; psoriasis
doi：10.4268/cjcmm20150530
银屑病(psoriasis)是一种免疫性皮肤病，以表皮过度增殖、慢性炎症和T淋巴细胞浸润为主要特征。目前，IL-23/Th17轴被认为是银屑病发生的重要环节。Th17细胞分泌的主要效应细胞因子即IL-17，能够募集中性粒细胞和参与炎症反应，在银屑病患者皮损呈阳性表达[1-3]。紫草素是清热凉血中药紫草的主要有效成分，紫草是治疗银屑病的常用中药之一。已有文献报道紫草素抑制角质形成细胞增殖的作用[4-5]。本研究通过建立IL-17A诱导HaCaT细胞增殖模型，观察紫草素对细胞增殖和分泌趋化因子的影响，尝试从炎症方面探讨紫草素治疗银屑病的可能作用机制。, 百拇医药(解欣然 张蕾 刘欣 林燕 张璐 李萍)