桂枝茯苓胶囊及其活性成分组合物抗炎作用与机制研究(1)
[摘要]该研究旨在脂多糖(LPS)刺激RAW264.7细胞模型上，对桂枝茯苓胶囊及活性成分组合物(15个成分组成)进行体外抗炎活性量效评价，并对其作用机制进行初步探索。采用MTT法检测桂枝茯苓胶囊及活性成分组合物对RAW264.7细胞活力的影响，ELISA法检测桂枝茯苓胶囊及活性成分组合物对LPS诱导RAW264.7细胞释放IL-1β，TNF-α和PGE2的影响，Western blot法检测桂枝茯苓胶囊及活性成分组合物对IL-1β和mPGES-1表达的影响。结果显示在测定浓度下，桂枝茯苓胶囊及活性成分组合物对RAW264.7细胞活力无显著影响;ELISA检测结果表明桂枝茯苓胶囊及活性成分组合物能有效抑制IL-1β，TNF-α和PGE2的释放，且均呈现一定的浓度-效应依赖关系;Western blot结果表明桂枝茯苓胶囊及活性成分组合物能有效降低IL-1β与mPGES-1的表达。以上结果表明桂枝茯苓胶囊及活性成分组合物能有效抑制LPS诱导的炎症因子释放，其作用机制可能与降低IL-1β与mPGES-1的表达有关。该研究为阐明桂枝茯苓胶囊的抗炎活性和相关机制奠定了实验基础，同时对桂枝茯苓胶囊的效应物质基础解析提供了一定的理论依据。
, 百拇医药
[关键词]桂枝茯苓胶囊;脂多糖;抗炎
Study on anti-inflammation effect and involved mechanism of Guizhi Fuling
capsule and its active complex
ZHANG Zhen-zhen1，2， ZHANG Xin-zhuang1，2， LI Na1，2， CAO Liang1，2， DING Gang1，2， WANG Zhen-zhong1，2， XIAO Wei1，2*
(1. Jiangsu Kanion Pharmaceutical Co.， Ltd.， Lianyungan 222001， China;
2. State Key Laboratory of Pharmaceutical Process New-tech for Chinese Medicine， Lianyungang 222001， China)
, 百拇医药
[Abstract]The aim of this study was to investigate the anti-inflammatory effect of Guizhi Fuling capsule and its active complex (consistent of 15 active compounds) on LPS-induced RAW264.7 cells. The effect of Guizhi Fuling capsule and its active complex on cell viability in RAW264.7 cells were determined by MTT assay. The inhibitory effect of Guizhi Fuling capsule and active complex on the releasing of IL-1β， TNF-αand PGE2 induced by LPS in RAW264.7 cells was detected by ELISA assay. The expression of IL-1β and mPGES-1 in Guizhi Fuling capsule or active complex treated RAW264.7 cells was examined by Western blot assay. Guizhi Fuling capsule and active complex showed no significant effect on the cell viability in RAW264.7 cells at doses range from 12.5 to 400 mg·L-1. Compared with LPS treated group， Guizhi Fuling capsule and active complex dose dependently reduced the releasing of IL-1β， TNF-α and PGE2 induced by LPS in RAW264.7 cells. Moreover， the expression of IL-1β and mPGES-1 was decreased after Guizhi Fuling capsule and active complex treatment， which might contribute to the inhibitory effect of Guizhi Fuling capsule in the releasing of IL-1β， TNF-α and PGE2. This study provided the evidence that Guizhi Fuling capsule and active complex remarkably inhibited the releasing of IL-1β， TNF-α and PGE2 induced by LPS in RAW264.7 cells by reducing the expression IL-1β and mPGES-1.This study provided an experimental basis of Guizhi Fuling capsule for the treatment of inflammation and a theoretical basis for the development of effective compounds of Guizhi Fuling capsule., http://www.100md.com(张珍珍 张新庄 李娜 曹亮 丁岗 王振中 萧伟)
, 百拇医药
[关键词]桂枝茯苓胶囊;脂多糖;抗炎
Study on anti-inflammation effect and involved mechanism of Guizhi Fuling
capsule and its active complex
ZHANG Zhen-zhen1，2， ZHANG Xin-zhuang1，2， LI Na1，2， CAO Liang1，2， DING Gang1，2， WANG Zhen-zhong1，2， XIAO Wei1，2*
(1. Jiangsu Kanion Pharmaceutical Co.， Ltd.， Lianyungan 222001， China;
2. State Key Laboratory of Pharmaceutical Process New-tech for Chinese Medicine， Lianyungang 222001， China)
, 百拇医药
[Abstract]The aim of this study was to investigate the anti-inflammatory effect of Guizhi Fuling capsule and its active complex (consistent of 15 active compounds) on LPS-induced RAW264.7 cells. The effect of Guizhi Fuling capsule and its active complex on cell viability in RAW264.7 cells were determined by MTT assay. The inhibitory effect of Guizhi Fuling capsule and active complex on the releasing of IL-1β， TNF-αand PGE2 induced by LPS in RAW264.7 cells was detected by ELISA assay. The expression of IL-1β and mPGES-1 in Guizhi Fuling capsule or active complex treated RAW264.7 cells was examined by Western blot assay. Guizhi Fuling capsule and active complex showed no significant effect on the cell viability in RAW264.7 cells at doses range from 12.5 to 400 mg·L-1. Compared with LPS treated group， Guizhi Fuling capsule and active complex dose dependently reduced the releasing of IL-1β， TNF-α and PGE2 induced by LPS in RAW264.7 cells. Moreover， the expression of IL-1β and mPGES-1 was decreased after Guizhi Fuling capsule and active complex treatment， which might contribute to the inhibitory effect of Guizhi Fuling capsule in the releasing of IL-1β， TNF-α and PGE2. This study provided the evidence that Guizhi Fuling capsule and active complex remarkably inhibited the releasing of IL-1β， TNF-α and PGE2 induced by LPS in RAW264.7 cells by reducing the expression IL-1β and mPGES-1.This study provided an experimental basis of Guizhi Fuling capsule for the treatment of inflammation and a theoretical basis for the development of effective compounds of Guizhi Fuling capsule., http://www.100md.com(张珍珍 张新庄 李娜 曹亮 丁岗 王振中 萧伟)