金线莲基原植物花粉活力和柱头可授性及结实特征研究(1)
[摘要]采用TTC法和过氧化物酶法测定花粉活力及寿命，用联苯胺-过氧化氢法测定柱头可授性，并对不同授粉方式的植株进行结实特征调查。结果表明：①金线兰与台湾金线莲在花粉活力上表现出相同的趋势，先上升后下降;贮藏温度与贮藏时间对金线莲花粉活力存在显著的影响，随着贮藏时间的延长，花粉活力降低，4 ℃冰箱贮藏可适当延长花粉活力。②柱头于开花当天就具有可授性，第4天最强，随后逐渐降低，金线兰于花后第8天不具可授性，而台湾金线莲于花后第10天不具可授性。③不同授粉方式对金线莲结实率影响显著，人工异花授粉的结实率远远高于人工自花授粉。因此，采集花后第3天花粉对花后第4天的柱头进行人工异花授粉杂交，可显著提高结实率，为金线莲新品种的选育和良种繁育提供技术保证。
[关键词]金线兰;台湾金线莲;花粉活力;柱头可授性;结实
Pollen viability， stigma receptivity and fruiting characteristics of
, 百拇医药
botanical origin of Jinxianlian
SHAO Qing-song/1， WANG Yong/1， HU Run-huai1*， ZHANG Wang-shu/1， LI Ming-yan/1
(1. Nurturing Station for the State Key Laboratory of Subtropical Silviculture， Zhejiang A & F University， Hangzhou 311300， China;
2. Modern Agricultural Research Center in Ningbo， Industrial Technology Research Institute of Zhejiang University，Ningbo 315033， China; 3. Rare Herbal Medicine and Engineering Institute of Zhejiang Province， Wuyi 321200， China)
, 百拇医药
[Abstract]The viability and life span of pollen were evaluated by TTC (2，3，5-triphenyl tetrazlium chloride) and the peroxidase solution， the stigma receptivity were estimated by benzidine-H2O2 method and the fruiting characteristics were investigated. The results showed that ①Anoectochilus roxburghii and A. formosanus appeared the same up-and-down trend of the pollen viability， increased and then decreased. The storage temperature and storage time had significant impact on the pollen viability. With the extension of storage time， the pollen activity decreased. 4 ℃ refrigerator storage may be extended the pollen vitality. ②The stigma had receptivity in 1st day and reached the highest level in the 4th day after blooming. A. roxburghii lost receptivity in the 8th day while A. formosanus lost receptivity in the 10th day after blooming.③The different pollination had significant impact on seed setting rate. The seed setting rate of artificial cross-pollination was higher than that of the artificial self-pollination. Collecting pollen in the 3rd day and carrying out artificial cross-pollination in the 4th day after blooming can significantly improve seed setting rate. The results provided technical assurance for A. roxburghii and A. formosanus breeding of new varieties and seed breeding.
[Key words]Anoectochilus roxburghii; Anoectochilus formosanus; pollen viability; stigma receptivity; seed
doi：10.4268/cjcmm20150614, http://www.100md.com(邵清松 王勇 胡润淮 张望舒 李明焱)
[关键词]金线兰;台湾金线莲;花粉活力;柱头可授性;结实
Pollen viability， stigma receptivity and fruiting characteristics of
, 百拇医药
botanical origin of Jinxianlian
SHAO Qing-song/1， WANG Yong/1， HU Run-huai1*， ZHANG Wang-shu/1， LI Ming-yan/1
(1. Nurturing Station for the State Key Laboratory of Subtropical Silviculture， Zhejiang A & F University， Hangzhou 311300， China;
2. Modern Agricultural Research Center in Ningbo， Industrial Technology Research Institute of Zhejiang University，Ningbo 315033， China; 3. Rare Herbal Medicine and Engineering Institute of Zhejiang Province， Wuyi 321200， China)
, 百拇医药
[Abstract]The viability and life span of pollen were evaluated by TTC (2，3，5-triphenyl tetrazlium chloride) and the peroxidase solution， the stigma receptivity were estimated by benzidine-H2O2 method and the fruiting characteristics were investigated. The results showed that ①Anoectochilus roxburghii and A. formosanus appeared the same up-and-down trend of the pollen viability， increased and then decreased. The storage temperature and storage time had significant impact on the pollen viability. With the extension of storage time， the pollen activity decreased. 4 ℃ refrigerator storage may be extended the pollen vitality. ②The stigma had receptivity in 1st day and reached the highest level in the 4th day after blooming. A. roxburghii lost receptivity in the 8th day while A. formosanus lost receptivity in the 10th day after blooming.③The different pollination had significant impact on seed setting rate. The seed setting rate of artificial cross-pollination was higher than that of the artificial self-pollination. Collecting pollen in the 3rd day and carrying out artificial cross-pollination in the 4th day after blooming can significantly improve seed setting rate. The results provided technical assurance for A. roxburghii and A. formosanus breeding of new varieties and seed breeding.
[Key words]Anoectochilus roxburghii; Anoectochilus formosanus; pollen viability; stigma receptivity; seed
doi：10.4268/cjcmm20150614, http://www.100md.com(邵清松 王勇 胡润淮 张望舒 李明焱)