基于剂量的雷公藤抗小鼠免疫性炎症“效毒”关联性评价(1)
[摘要]目的：研究雷公藤水煎液发挥抗免疫炎症药效同时产生的伴随毒性作用，为其“药效-毒性”相关性研究提供实验依据。方法：建立二硝基氟苯诱导小鼠迟发型超敏反应模型，连续给予不同剂量的雷公藤水煎液5d，观察小鼠耳肿胀抑制率及其毒副作用，于末次给药后检测血中PGE2，TNF-α，IL-2，ALT，AST，PA，TBA，TBIL等生化指标水平的变化，并计算心、肝、肾、脾、肾等脏器指数。结果：连续多次给小鼠灌胃雷公藤水煎液可明显抑制DNFB致小鼠耳肿胀，其ED50及其95%可信限为0.34(0.21～0.42)g·kg-1。血中PGE2，TNF-α，IL-2含量降低，呈现一定的剂量依赖关系;小鼠血中AST，ALT，TBA，TBIL活性升高，PA含量降低。结论：雷公藤水煎液在0.59～2.34g·kg-1具有较强的抗免疫炎症作用，呈现“量-效”关系，同时可对小鼠肝脏产生一定的毒副作用，高剂量(2.34～4.68g·kg-1)可引起实质性肝损伤，肝功能指标呈现剂量依赖性。因此，雷公藤的“功效-毒性”具有一定的依赖于剂量的相关性，为今后临床预防药品不良反应的预警方案提供参考，确保雷公藤临床用药安全。
, 百拇医药
[关键词]雷公藤;剂量;免疫炎症;功效;毒性
Evaluationondosage-basedefficacy-toxicitycorrelationofTripterygium
wilfordiiagainstimmuneinflammationinmice
ZHAOQing-hua1，2，LIXiao-yu/1，FENGQun/1，SUNRong1*
(1.ShandongAcademyofChineseMedicine，Ji′nan250014，China;2.ShandongUniversityofTraditionalChineseMedicine，Ji′nan250355，China;3.TianjinUniversityofTraditionalChineseMedicine，Tianjin300193，China)
, http://www.100md.com
[Abstract]Objective：Tostudytheanti-immuneinflammationefficacyandtoxicityofTripterygiumwilfordiidecoction，inordertoprovideexperimentalbasisforstudiesonits″efficacy-toxicity″correlation.Method：Thedelayedhypersensitivitymodelwasestablishedbydinitrofluorobenzeneinmice.DifferentdosesofT.wilfordiidecoctionwasadministeredfor5consecutivedays.Theearswellinginhibitionratioandthetoxicactionwereobserved.Afterthefinaladministration，thebiochemicalindexesofPGE2，TNF-α，IL-2，ALT，AST，PA，TBA，TBILinserumweredetected，andthevisceralindexesofheart，liver，spleenandkidneyweremeasured.Result：TheDNFB-inducedearswellingcouldbenotablyinhibitedbymultipleoraladministrationofT.wilfordiidecoction，withtheED50andits95%confidencelimitof0.34(0.21-0.42)g·kg-1.ThecontentsofPGE2，TNF-α，IL-2inserumdecreasedinadose-dependentmanner.TheactivitiesofserumAST，ALT，TBA，TBILandthePAcontentreduced.Conclusion：T.wilfordiidecoctionshowsasignificantanti-immuneinflammationefficacywithinthedosagerangebetween0.59and2.34g·kg-1inadose-dependentmanner.Withacertainhepatotoxicity，highdose(2.34-4.68g·kg-1)ofT.wilfordiidecoctioncancausesubstantialliverinjury，withadosedependenceinliverfunctionindex.Therefore，theefficacyandtoxicityofT.wilfordiiisdosedependent，whichprovidesreferenceforpreventingadversedrugreactionsinclinicanddevelopingearly-warningschemesandensuretheclinicalmedicationsafetyofT.wilfordii.
[Keywords]Tripterygiumwilfordii;dose;immuneinflammation;efficacy;toxicity, http://www.100md.com(赵庆华 李晓宇 冯群 孙蓉)
, 百拇医药
[关键词]雷公藤;剂量;免疫炎症;功效;毒性
Evaluationondosage-basedefficacy-toxicitycorrelationofTripterygium
wilfordiiagainstimmuneinflammationinmice
ZHAOQing-hua1，2，LIXiao-yu/1，FENGQun/1，SUNRong1*
(1.ShandongAcademyofChineseMedicine，Ji′nan250014，China;2.ShandongUniversityofTraditionalChineseMedicine，Ji′nan250355，China;3.TianjinUniversityofTraditionalChineseMedicine，Tianjin300193，China)
, http://www.100md.com
[Abstract]Objective：Tostudytheanti-immuneinflammationefficacyandtoxicityofTripterygiumwilfordiidecoction，inordertoprovideexperimentalbasisforstudiesonits″efficacy-toxicity″correlation.Method：Thedelayedhypersensitivitymodelwasestablishedbydinitrofluorobenzeneinmice.DifferentdosesofT.wilfordiidecoctionwasadministeredfor5consecutivedays.Theearswellinginhibitionratioandthetoxicactionwereobserved.Afterthefinaladministration，thebiochemicalindexesofPGE2，TNF-α，IL-2，ALT，AST，PA，TBA，TBILinserumweredetected，andthevisceralindexesofheart，liver，spleenandkidneyweremeasured.Result：TheDNFB-inducedearswellingcouldbenotablyinhibitedbymultipleoraladministrationofT.wilfordiidecoction，withtheED50andits95%confidencelimitof0.34(0.21-0.42)g·kg-1.ThecontentsofPGE2，TNF-α，IL-2inserumdecreasedinadose-dependentmanner.TheactivitiesofserumAST，ALT，TBA，TBILandthePAcontentreduced.Conclusion：T.wilfordiidecoctionshowsasignificantanti-immuneinflammationefficacywithinthedosagerangebetween0.59and2.34g·kg-1inadose-dependentmanner.Withacertainhepatotoxicity，highdose(2.34-4.68g·kg-1)ofT.wilfordiidecoctioncancausesubstantialliverinjury，withadosedependenceinliverfunctionindex.Therefore，theefficacyandtoxicityofT.wilfordiiisdosedependent，whichprovidesreferenceforpreventingadversedrugreactionsinclinicanddevelopingearly-warningschemesandensuretheclinicalmedicationsafetyofT.wilfordii.
[Keywords]Tripterygiumwilfordii;dose;immuneinflammation;efficacy;toxicity, http://www.100md.com(赵庆华 李晓宇 冯群 孙蓉)