壮腰健肾丸对肾虚多尿大鼠膀胱组织中P2X1与P2X3 mRNA表达的影响(1)
[摘要] 为了探讨壮腰健肾丸的缩尿作用及机制，该实验采用皮下注射150 mg·kg-1剂量的D-半乳糖诱导亚急性衰老模型，并将其分为模型组、缩泉丸组(1.17 g·kg-1·d-1)、壮腰健肾丸高、中、低剂量组(2.39，1.20，0.60 g·kg-1·d-1)，另取正常动物设为空白组，连续给药8周。选用代谢笼法测量大鼠24 h尿量及水负荷5 h尿量;采用全自动生化仪检测尿中Na+，Cl-，K+浓度;应用ELISA法测定血清中醛固酮(aldosterone，ALD)、抗利尿激素(antidiuretic hormone，ADH)的含量;采用RT-PCR检测大鼠膀胱组织中P2X1与P2X3 mRNA表达量的变化情况。结果显示壮腰健肾丸高、中剂量组对肾虚多尿大鼠24 h尿量及水负荷后5 h内尿量均显著下调(P<0.05，P<0.01);对尿中Na+和Cl-浓度均显著降低(P<0.01);血浆中ALD，ADH含量显著性升高(P<0.05，P<0.01);膀胱组织中P2X1与P2X3 mRNA表达量显著下调(P<0.01);壮腰健肾丸低剂量组尿中Na+和Cl-浓度均显著降低(P<0.01)。结果说明壮腰健肾丸可能是通过下调肾虚多尿大鼠膀胱组织中P2X1与P2X3 mRNA表达量从而发挥缩尿的作用。
[关键词] 壮腰健肾丸;肾虚多尿;P2X1;P2X3
[收稿日期] 2014-12-08
[基金项目] 中央财政支持地方高校发展专项资金项目 (财教[2013]338号)
[通信作者] *操红缨，副教授，医学博士，主要从事中药复方药理研究，E-mail：hycao@gzhtcm.edu.cn
[作者简介] 陈家艺，硕士研究生，主要从事中药新药与保健品研发，E-mail：13694237210@163.com
Effect of Zhuangyao Jianshen Wan (ZYJCW) on P2X1 and P2X3
mRNA expressions in rats with diuresis caused by kidney deficiency
CHEN Jia-yi1， JIANG Wei-wen2， HE Feng-lei3， MO Guo-qiang3， GUO Zhong-hui3， WANG Xiao-dan3， WU Qing-he2， CAO Hong-yin2*
(1.University Hospital of Hubei University for Nationalities， Ehshi 445000， China;
2. Guangzhou University of Chinese Medicine， Guangzhou 510405， China;
3. Guangzhou Baiyunshan Pharmaceutical Co. Chenliji， Guangzhou 510290， China)
[Abstract] To investigate the urination-reducing effect and mechanism of Zhuangyao Jianshen Wan (ZYJCW). In this study， SD rats were subcutaneously injected with 150 mg·kg-1 dose of D-galactose to prepare the sub-acute aging model and randomly divided into the model group， the Suoquan Wan group (1.17 g·kg-1·d-1)， and ZYJCW high， medium and low dose groups (2.39， 1.20， 0.60 g·kg-1·d-1)， with normal rats in the blank group. They were continuously administered with drugs for eight weeks. The metabolic cage method was adopted to measure the 24 h urine volume and 5 h water load urine volume in rats. The automatic biochemistry analyzer was adopted to detect urine concentrations of Na+， Cl-， K+. The ELISA method was used to determine serum aldosterone (ALD) and antidiuretic hormone (ADH). The changes in P2X1 and P2X3 mRNA expressions in bladder tissues of rats were detected by RT-PCR. According to the results， both ZYJCW high and medium dose groups showed significant down-regulations in 24 h urine volume and 5 h water load urine volume in (P<0.05， P<0.01)， declines in Na+ and Cl- concentrations in urine (P<0.01)， notable rises in plasma ALD and ADH contents (P<0.05， P<0.01) and remarkable down-regulations in the P2X1 and P2X3 mRNA expressions in bladder tissues (P<0.01). The ZYJCW low dose group revealed obvious reductions in Na+ and Cl- concentrations in urine(P<0.01). The results indicated that ZYJCW may show the urination-reducing effect by down-regulating the P2X1 and P2X3 mRNA expressions in bladder tissues of rats with diuresis caused by kidney deficiency. (陈家艺 江伟雯 何风雷 莫国强 郭钟慧王笑丹 吴清和 操红缨)
[关键词] 壮腰健肾丸;肾虚多尿;P2X1;P2X3
[收稿日期] 2014-12-08
[基金项目] 中央财政支持地方高校发展专项资金项目 (财教[2013]338号)
[通信作者] *操红缨，副教授，医学博士，主要从事中药复方药理研究，E-mail：hycao@gzhtcm.edu.cn
[作者简介] 陈家艺，硕士研究生，主要从事中药新药与保健品研发，E-mail：13694237210@163.com
Effect of Zhuangyao Jianshen Wan (ZYJCW) on P2X1 and P2X3
mRNA expressions in rats with diuresis caused by kidney deficiency
CHEN Jia-yi1， JIANG Wei-wen2， HE Feng-lei3， MO Guo-qiang3， GUO Zhong-hui3， WANG Xiao-dan3， WU Qing-he2， CAO Hong-yin2*
(1.University Hospital of Hubei University for Nationalities， Ehshi 445000， China;
2. Guangzhou University of Chinese Medicine， Guangzhou 510405， China;
3. Guangzhou Baiyunshan Pharmaceutical Co. Chenliji， Guangzhou 510290， China)
[Abstract] To investigate the urination-reducing effect and mechanism of Zhuangyao Jianshen Wan (ZYJCW). In this study， SD rats were subcutaneously injected with 150 mg·kg-1 dose of D-galactose to prepare the sub-acute aging model and randomly divided into the model group， the Suoquan Wan group (1.17 g·kg-1·d-1)， and ZYJCW high， medium and low dose groups (2.39， 1.20， 0.60 g·kg-1·d-1)， with normal rats in the blank group. They were continuously administered with drugs for eight weeks. The metabolic cage method was adopted to measure the 24 h urine volume and 5 h water load urine volume in rats. The automatic biochemistry analyzer was adopted to detect urine concentrations of Na+， Cl-， K+. The ELISA method was used to determine serum aldosterone (ALD) and antidiuretic hormone (ADH). The changes in P2X1 and P2X3 mRNA expressions in bladder tissues of rats were detected by RT-PCR. According to the results， both ZYJCW high and medium dose groups showed significant down-regulations in 24 h urine volume and 5 h water load urine volume in (P<0.05， P<0.01)， declines in Na+ and Cl- concentrations in urine (P<0.01)， notable rises in plasma ALD and ADH contents (P<0.05， P<0.01) and remarkable down-regulations in the P2X1 and P2X3 mRNA expressions in bladder tissues (P<0.01). The ZYJCW low dose group revealed obvious reductions in Na+ and Cl- concentrations in urine(P<0.01). The results indicated that ZYJCW may show the urination-reducing effect by down-regulating the P2X1 and P2X3 mRNA expressions in bladder tissues of rats with diuresis caused by kidney deficiency. (陈家艺 江伟雯 何风雷 莫国强 郭钟慧王笑丹 吴清和 操红缨)