铁皮石斛内生真菌对宿主组培苗生长与代谢成分的影响(1)
[摘要]为揭示初筛所得7株活树附生铁皮石斛内生真菌对宿主生长及代谢成分的影响，该研究观察测量了接菌与未接菌铁皮石斛组培苗形态特征与农艺性状，采用苯酚硫酸法与热浸法测定组培苗茎总多糖含量与浸出物含量，柱前衍生HPLC分析茎多糖中单糖组成，HPLC分析茎醇溶性成分。研究表明，供试7个菌株可改变铁皮石斛组培苗茎节变紫进程，并影响茎糖类及醇溶性成分组成及含量积累，除DO34外均能促进组培苗生长与提高多糖含量，除DO12外均能促进甘露糖含量积累，DO18，DO19，DO120可促进醇溶性浸出物含量积累，综合生长及代谢成分多项指标筛选出DO14，DO18，DO19与DO120 4个优良菌株，为铁皮石斛内生真菌和宿主相互作用机制研究与微生物工程研究提供了菌种材料与研究基础。
[关键词]铁皮石斛； 内生真菌； 生长发育； 代谢成分
[Abstract]The paper aims to study the effects of endophytic fungi from D officinale cultivated on living trees on growth and components metabolism of tissue culture seedlings Morphological characteristics and agronomic characters of tissue culture seedlings infected and uninfected by endophytic fungus were observed and measured Polysaccharides and alcoholsoluble extracts contents were determined by phenolsulfuric acid method and hotdipmethod ......
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[关键词]铁皮石斛； 内生真菌； 生长发育； 代谢成分
[Abstract]The paper aims to study the effects of endophytic fungi from D officinale cultivated on living trees on growth and components metabolism of tissue culture seedlings Morphological characteristics and agronomic characters of tissue culture seedlings infected and uninfected by endophytic fungus were observed and measured Polysaccharides and alcoholsoluble extracts contents were determined by phenolsulfuric acid method and hotdipmethod ......
您现在查看是摘要页，全文长 3532 字符。