酸枣仁皂苷A人工抗原的制备及鉴定(1)
[摘要]采用高碘酸钠氧化法首次合成酸枣仁皂苷A(jujuboside A)人工抗原，利用基质辅助激光解析电离飞行时间质谱法(MALDITOFMS)鉴定酸枣仁皂苷A人工抗原偶联成功，通过酶联免疫吸附测定法(enzymelinked immunosorbent assay，ELISA)检测免疫小鼠血清中抗酸枣仁皂苷A抗体效价及其特异性。竞争抑制曲线表明，血清中的抗体可与酸枣仁皂苷A产生特异性结合，血清抗体效价在1∶4 000。成功合成的酸枣仁皂苷A人工抗原，为酸枣仁皂苷A单克隆抗体的制备及研究酸枣仁皂苷A在机体内的药物代谢过程奠定了基础。
[关键词]酸枣仁皂苷A；人工抗原；高碘酸钠法；单克隆抗体；基质辅助激光解析电离飞行时间质谱法
[Abstract]Immunogenic antigen (jujuboside ABSA) and coating antigen (jujuboside AOVA) of jujuboside A were synthesized by sodium periodate oxidation method for the first time Jujuboside A artificial antigen was confirmed by matrixassisted laser desorption ionization/timeofflight mass spectrometry (MALDITOFMS) The titer and specificity of the antibody in serum of immunized mice were detected by enzymelinked immunosorbent assay (ELISA) The corrected relation curve of inhibition rate showed that the antibody against Jujuboside A obtained from immunized mice could bind to jujuboside A and the titer was up to 1∶4 000 The jujuboside A artificial antigen was synthesized ......
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[关键词]酸枣仁皂苷A；人工抗原；高碘酸钠法；单克隆抗体；基质辅助激光解析电离飞行时间质谱法
[Abstract]Immunogenic antigen (jujuboside ABSA) and coating antigen (jujuboside AOVA) of jujuboside A were synthesized by sodium periodate oxidation method for the first time Jujuboside A artificial antigen was confirmed by matrixassisted laser desorption ionization/timeofflight mass spectrometry (MALDITOFMS) The titer and specificity of the antibody in serum of immunized mice were detected by enzymelinked immunosorbent assay (ELISA) The corrected relation curve of inhibition rate showed that the antibody against Jujuboside A obtained from immunized mice could bind to jujuboside A and the titer was up to 1∶4 000 The jujuboside A artificial antigen was synthesized ......
您现在查看是摘要页，全文长 3490 字符。