妇科千金片对阿奇霉素在慢性盆腔炎大鼠体内药物动力学的影响(1)
[摘要] 研究妇科千金片对阿奇霉素(AZM)在慢性盆腔炎大鼠体内多次给药药物动力学的影响，评价联合用药的合理性。以雌性SD大鼠为实验对象，采用子宫内接种混合菌液加机械损伤法建立慢性盆腔炎动物模型。将模型大鼠随机分为对照组和联合用药组，对照组灌胃给予阿奇霉素10 mg·kg-1·d-1，联合用药组给予等剂量阿奇霉素2 h后灌胃妇科千金片166 g·kg-1·d-1，每日1次，连续7 d。于不同时间点采集血浆样本，HPLCMS测定血浆中阿奇霉素浓度。采用ADAPT 51软件反向高斯模型计算药动学参数，SPSS软件对各组参数进行统计学分析。结果显示妇科千金片对阿奇霉素的药动学参数CLt，CLd，MIT，F均无显著影响，研究表明临床妇科千金片与阿奇霉素联合应用于慢性盆腔炎的治疗具有一定的合理性。
[关键词] 妇科千金片； 阿奇霉素； 多次给药； 药动学； 联合用药
Effect of Fuke Qianjin tablets on pharmacokinetics of Azithromycin in
rats with chronic pelvic inflammatory disease
LI Wei1， ZOU Liang1*， GONG Yun2， ZHANG Peng2， WANG Ping3， ZHANG Yan3， QIU Lu1
(1 Chengdu University， Chengdu 610106， China；
2 Zhuzhou Qianjin Pharmaceutical Co， Ltd， Zhuzhou 412003， China；
3 Chengdu University of Traditional Chinese Medicine， Chengdu 611137， China)
[Abstract] To investigate the effect of Fuke Qianjin tablets on the pharmacokinetics of Azithromycin(AZM) in rats with chronic pelvic inflammation， and evaluate the rationality of the drug combination The animal models of chronic pelvic inflammatory disease were established by intrauterine injection of mixed bacterial suspension plus mechanic injury in female SpragueDawley(SD) rats The model rats were randomly divided into control group and drug combination group The rats in control group were given with Azithromycin 10 mg·kg1·d1 by intragastric administration， while the rats in drug combination group were given with Fuke Qianjin tablets 166 g·kg1·d1by intragastric administration 2 hours after the equivalent dose of azithromycin， once a day， consecutively for 7 days Plasma samples were collected at different time points and the concentration of AZM in plasma was determined by high performance liquid chromatography coupled with mass spectrometry(HPLCMS) ADAPT 51 software was used to calculate the pharmacokinetic parameters of each group by Inverse Gaussian Function model SPSS software was used for the statistical analysis of data in each group The results showed that Fuke Qianjin tablets had no significant effects on the main pharmacokinetic parameters of AZM， including CLt， CLd， MIT and F The study showed that Fuke Qianjin tablets can be combined with azithromycin for treatment of chronic pelvic inflammation disease
[Key words] Fuke Qianjin tablets； Azithromycin； multiple dosing； pharmacokinetics； drug combination
doi：10.4268/cjcmm20161227
慢性盆腔炎(chronic pelvic inflammatory disease，CPID)为妇科常见病，临床主要表现为下腹坠痛、腰骶酸痛等。其发病机制较为复杂，现代医学研究认为该病主要是由致病菌引起，以抗生素治疗为主。我国传统医学认为湿热瘀结、气滞血瘀是导致该病的主要原因。由于部分慢性盆腔炎发展过程中并无病原体存在[1]，同时抗生素常具有耐药性，因此西医学单独应用抗生素治疗的效果并非特别理想。近年来，随着中医药现代化进程加速，中西药联合应用日趋广泛，临床对慢性盆腔炎的治疗亦常采用中西医结合疗法[23]或中药与抗生素联合应用[4]。有研究报道妇科千金片与抗生素联合应用治疗慢性盆腔炎疗效显著提高，且不良反应发生率明显降低[57]。妇科千金片由千斤拔、穿心莲、金樱根、功劳木、单面针、当归、鸡血藤、党参8味药组成，具有清热除湿、益气化瘀之效，为临床治疗慢性盆腔炎的常用药。妇科千金片联合阿奇霉素对慢性妇科炎症的疗效优于单用阿奇霉素，因此临床上常将两者联合应用于治疗慢性妇科炎症疾病[8]。 (李维 邹亮 龚云 张鹏 王平 张艳 邱露)
[关键词] 妇科千金片； 阿奇霉素； 多次给药； 药动学； 联合用药
Effect of Fuke Qianjin tablets on pharmacokinetics of Azithromycin in
rats with chronic pelvic inflammatory disease
LI Wei1， ZOU Liang1*， GONG Yun2， ZHANG Peng2， WANG Ping3， ZHANG Yan3， QIU Lu1
(1 Chengdu University， Chengdu 610106， China；
2 Zhuzhou Qianjin Pharmaceutical Co， Ltd， Zhuzhou 412003， China；
3 Chengdu University of Traditional Chinese Medicine， Chengdu 611137， China)
[Abstract] To investigate the effect of Fuke Qianjin tablets on the pharmacokinetics of Azithromycin(AZM) in rats with chronic pelvic inflammation， and evaluate the rationality of the drug combination The animal models of chronic pelvic inflammatory disease were established by intrauterine injection of mixed bacterial suspension plus mechanic injury in female SpragueDawley(SD) rats The model rats were randomly divided into control group and drug combination group The rats in control group were given with Azithromycin 10 mg·kg1·d1 by intragastric administration， while the rats in drug combination group were given with Fuke Qianjin tablets 166 g·kg1·d1by intragastric administration 2 hours after the equivalent dose of azithromycin， once a day， consecutively for 7 days Plasma samples were collected at different time points and the concentration of AZM in plasma was determined by high performance liquid chromatography coupled with mass spectrometry(HPLCMS) ADAPT 51 software was used to calculate the pharmacokinetic parameters of each group by Inverse Gaussian Function model SPSS software was used for the statistical analysis of data in each group The results showed that Fuke Qianjin tablets had no significant effects on the main pharmacokinetic parameters of AZM， including CLt， CLd， MIT and F The study showed that Fuke Qianjin tablets can be combined with azithromycin for treatment of chronic pelvic inflammation disease
[Key words] Fuke Qianjin tablets； Azithromycin； multiple dosing； pharmacokinetics； drug combination
doi：10.4268/cjcmm20161227
慢性盆腔炎(chronic pelvic inflammatory disease，CPID)为妇科常见病，临床主要表现为下腹坠痛、腰骶酸痛等。其发病机制较为复杂，现代医学研究认为该病主要是由致病菌引起，以抗生素治疗为主。我国传统医学认为湿热瘀结、气滞血瘀是导致该病的主要原因。由于部分慢性盆腔炎发展过程中并无病原体存在[1]，同时抗生素常具有耐药性，因此西医学单独应用抗生素治疗的效果并非特别理想。近年来，随着中医药现代化进程加速，中西药联合应用日趋广泛，临床对慢性盆腔炎的治疗亦常采用中西医结合疗法[23]或中药与抗生素联合应用[4]。有研究报道妇科千金片与抗生素联合应用治疗慢性盆腔炎疗效显著提高，且不良反应发生率明显降低[57]。妇科千金片由千斤拔、穿心莲、金樱根、功劳木、单面针、当归、鸡血藤、党参8味药组成，具有清热除湿、益气化瘀之效，为临床治疗慢性盆腔炎的常用药。妇科千金片联合阿奇霉素对慢性妇科炎症的疗效优于单用阿奇霉素，因此临床上常将两者联合应用于治疗慢性妇科炎症疾病[8]。 (李维 邹亮 龚云 张鹏 王平 张艳 邱露)