瓜蒌薤白半夏汤对ApoE小鼠血脂代谢、氧化应激和主动脉Lox—1表达的影响(1)
[摘要] 观察瓜蒌薤白半夏汤(GXBD)在调节血脂代谢、抗氧化、干预ox-LDL/Lox-1通路的作用，探讨其抗动脉粥样硬化(AS)的作用机制。以高脂饲料饲喂雄性Apo-E-/-小鼠42只，建立AS模型。AS模型小鼠随机分为模型组、辛伐他汀组、GXBD高、低剂量组，以C57BL/6J雄性小鼠作为正常对照组；每组10只，连续灌胃给药8周。末次给药后24 h，检测血清TC，TG，LDL-C，HDL-C及SOD，MDA，GSH-px，ox-LDL水平；HE染色觀察主动脉组织结构变化，Western blot和PCR分别检测主动脉Lox-1蛋白表达和mRNA水平。结果显示模型组小鼠血脂指标含量及血清MDA，ox-LDL水平显著高于正常对照组，SOD，GSH-px显著低于正常对照组，主动脉Lox-1蛋白表达水平及其mRNA水平显著高于正常对照组(P<0.05)，模型组主动脉出现明显粥样硬化斑块；GXBD高、低剂量组和辛伐他汀组小鼠血脂指标含量及MDA，ox-LDL水平显著低于模型组，SOD，GSH-px显著高于模型组，主动脉Lox-1蛋白表达水平及其mRNA水平显著低于模型组(P<0.05)，主动脉组织粥样硬化病变明显减轻。说明调节血脂代谢，抗氧化、抑制Lox-1的表达、干预ox-LDL/Lox-1通路，可能是其抗AS的机制之一。
, 百拇医药
[关键词] 瓜蒌薤白半夏汤； 动脉粥样硬化； 抗氧化； 血脂； ox-LDL/Lox-1通路
[Abstract] To observe the functions of Gualou Xiebai Banxia decoction(GXBD) on regulating lipid metabolism， anti-oxidation， and interposing ox-LDL/Lox-1 pathway， and to explore its anti-atherosclerosis (AS) mechanisms. AS models were established by using 42 Apo-E-/- male mice with high fat diet. AS model mice were randomly divided into the model group， simvastatin group， and GXBD high and low dose groups. C57BL/6J male mice were used as the normal control group， n=10 and the treatment lasted for 8 weeks. The levels of TC， TG， LDL-C， HDL-C， SOD， MDA， GSH-px， and ox-LDL in blood serum were tested 24 h after the last administration. The changes of aortic tissues structure were observed by HE staining； the expression levels of Lox-1 protein and the expression levels of mRNA were detected by Western blot and PCR respectively.Results showed that the blood lipid levels and MDA， ox-LDL levels in blood serum of model group were significantly higher than those in the normal control group， but SOD， GSH-px levels were significantly lower than those in the normal control group， and the Lox-1 protein and mRNA expression levels were also significantly higher than those in the control group(P<0.05)， namely aortic atherosclerosis lesions were obvious in model group.The levels of blood lipid and MDA， ox-LDL of GXBD high and low dose groups and simvastatin group were significantly lower than those in model group， while SOD， GSH-px levels were significantly higher than those in model group， and Lox-1 protein and mRNA expression levels were significantly lower than those in model group(P<0.05)， namely the aortic atherosclerosis lesions were significantly relieved. The above results indicated that GXBD was capable of modulating blood lipid， anti-oxidation， and inhibiting the expression of Lox-1， and interposing ox-LDL/Lox-1 pathway in the AS model Apo-E-/- mice， which may be one of the mechanisms of anti-atherosclerosis., 百拇医药(郭建恩 米树斌 闫秀川 辛思源 高飞 梁广和 李静华)
, 百拇医药
[关键词] 瓜蒌薤白半夏汤； 动脉粥样硬化； 抗氧化； 血脂； ox-LDL/Lox-1通路
[Abstract] To observe the functions of Gualou Xiebai Banxia decoction(GXBD) on regulating lipid metabolism， anti-oxidation， and interposing ox-LDL/Lox-1 pathway， and to explore its anti-atherosclerosis (AS) mechanisms. AS models were established by using 42 Apo-E-/- male mice with high fat diet. AS model mice were randomly divided into the model group， simvastatin group， and GXBD high and low dose groups. C57BL/6J male mice were used as the normal control group， n=10 and the treatment lasted for 8 weeks. The levels of TC， TG， LDL-C， HDL-C， SOD， MDA， GSH-px， and ox-LDL in blood serum were tested 24 h after the last administration. The changes of aortic tissues structure were observed by HE staining； the expression levels of Lox-1 protein and the expression levels of mRNA were detected by Western blot and PCR respectively.Results showed that the blood lipid levels and MDA， ox-LDL levels in blood serum of model group were significantly higher than those in the normal control group， but SOD， GSH-px levels were significantly lower than those in the normal control group， and the Lox-1 protein and mRNA expression levels were also significantly higher than those in the control group(P<0.05)， namely aortic atherosclerosis lesions were obvious in model group.The levels of blood lipid and MDA， ox-LDL of GXBD high and low dose groups and simvastatin group were significantly lower than those in model group， while SOD， GSH-px levels were significantly higher than those in model group， and Lox-1 protein and mRNA expression levels were significantly lower than those in model group(P<0.05)， namely the aortic atherosclerosis lesions were significantly relieved. The above results indicated that GXBD was capable of modulating blood lipid， anti-oxidation， and inhibiting the expression of Lox-1， and interposing ox-LDL/Lox-1 pathway in the AS model Apo-E-/- mice， which may be one of the mechanisms of anti-atherosclerosis., 百拇医药(郭建恩 米树斌 闫秀川 辛思源 高飞 梁广和 李静华)