当归对阴虚哮喘Balb/c小鼠Th1/Th2及Th17/Treg的影响及机制研究(1)
[摘要] 当归是临床常用中药，具有补阴养血、活血润燥的功能。为研究当归对阴虚哮喘Balb/c小鼠Th1/Th2及Th17/Treg的影响及机制，该实验通过卵清白蛋白加甲状腺素复制阴虚哮喘Balb/c小鼠模型，造模同时分别给予地塞米松(DXM)、当归提取液、当归提取液+DXM灌胃处理，HE染色进行肺组织病理学检查，ELISA法检测支气管肺泡灌洗液(BALF)中IL-4，IL-17，IFN-γ，TGF-β水平及维甲酸受体相关孤儿受体(RORγt)的表达情况。结果显示，当归可显著减轻肺组织炎症浸润程度，升高IFN-γ/IL-4及TGF-β/IL-17的比值，降低肺组织中RORγt的表达强度，当归与DXM配伍的治疗效果最为显著。提示，当归可通过抑制肺组织RORγt表达、调节Th1/Th2及Th17/Treg的功能平衡，改善气道的炎症状态，发挥对阴虚型哮喘的治疗作用。
[关键词] 当归；阴虚哮喘；Balb/c小鼠；Th1/Th2；Th17/Treg
[Abstract] Angelicae Sinensis Radix， with nourishing Yin， promoting blood circulation， and moisturizing dryness functions， is commonly used in clinical medicine. In order to investigate the effects and mechanism of Angelica sinensis(AS) on Th1/Th2 and Th17/Treg in mice with asthma and Yin deficiency syndrome， asthmatic and Yin deficiency syndrome Balb/c mice models were established by injecting and inhaling ovalbumin(OVA) and thyroxin. The models were treated with dexamethasone(DXM)， AS extract and AS extract+DXM， respectively. Pathological examination of lung tissues was conducted by HE staining， and ELISA was used to detect the levels of IL-4， IL-17， IFN-γ， TGF-β as well as retinoic acid receptor-related orphan receptor (RORγt). Results showed that AS could significantly improve the situation of inflammation infiltration， increase ratios of IFN-γ/IL-4 and TGF-β/IL-17， decrease the levels of RORγt in lung tissues. The AS+DXM group showed a best treatment effect. The results indicated that AS played a therapeutic role for asthma with Yin deficiency syndrome and improved airway inflammation by inhibiting the expression of RORγt in lung tissues and regulating the balance of Th1/Th2 and Th17/Treg.
, 百拇医药
[Key words] Angelicae Sinensis Radix； asthma with Yin deficiency syndrome； Balb/c mouse； Th1/Th2； Th17/Treg
哮喘(asthma)是我國临床中常见的一种呼吸系统疾病，其临床发作具有周期性、反复性、迁延不愈的特点[1-2]。2013年哮喘流行病学调查数据显示，我国大陆地区哮喘总患病率高达1.24%，严重威胁公众健康[3]。研究表明，哮喘发病的主要病理学机制是辅助性T细胞(Th)亚群的免疫功能失调，其中，Th1/Th2与Th17/调节性T细胞(Treg)的功能平衡是影响呼吸道炎性因子分泌及促炎症反应的关键因素[4-5]。近年来，中药以其多途径、多靶点调节机体免疫功能的特点在临床哮喘的治疗中取得优势。当归(Angelica sinensis，AS)为补血养阴活血的常用中药之一，《本草经疏》记载当归有“主咳逆上气”之功效[6]，以往实验研究报道当归挥发油对支气管平滑肌有明显的解痉作用[7]。然而，当归对机体T细胞亚群的免疫调节机制目前尚未完全明确。本研究通过复制阴虚哮喘Balb/c小鼠模型，研究当归对Th1/Th2及Th17/Treg细胞因子的影响，探讨当归治疗哮喘的免疫调节机制，为当归用于临床哮喘的防治提供实验依据。
, 百拇医药
1 材料与方法
1.1 动物、药物与试剂 50只雌性Balb/c小鼠，周龄(7.2±0.8)周，体重(20.15±1.62) g，供应单位为北京中医药大学，合格证0014036，许可证号SCXK(京)2016-0024。当归提取液由上海和黄药业有限公司生产(国药准字Z31020419)。致敏试剂卵清白蛋白(OVA)购自美国Grade V Sigma公司(批号101069301)。甲状腺片购自山东中泰药业有限公司(国药准字H37021712，批号14080303)，地塞米松磷酸钠注射液(DXM)由河南科伦药业有限公司提供(国药准字H41023598，批号13011911)，ELISA细胞因子试剂盒购自R&D systems(美国)，免疫组化试剂盒购自北京博奥森生物技术有限公司(批号AD082522)。, http://www.100md.com(马婷婷 冯兴中 王学艳)
[关键词] 当归；阴虚哮喘；Balb/c小鼠；Th1/Th2；Th17/Treg
[Abstract] Angelicae Sinensis Radix， with nourishing Yin， promoting blood circulation， and moisturizing dryness functions， is commonly used in clinical medicine. In order to investigate the effects and mechanism of Angelica sinensis(AS) on Th1/Th2 and Th17/Treg in mice with asthma and Yin deficiency syndrome， asthmatic and Yin deficiency syndrome Balb/c mice models were established by injecting and inhaling ovalbumin(OVA) and thyroxin. The models were treated with dexamethasone(DXM)， AS extract and AS extract+DXM， respectively. Pathological examination of lung tissues was conducted by HE staining， and ELISA was used to detect the levels of IL-4， IL-17， IFN-γ， TGF-β as well as retinoic acid receptor-related orphan receptor (RORγt). Results showed that AS could significantly improve the situation of inflammation infiltration， increase ratios of IFN-γ/IL-4 and TGF-β/IL-17， decrease the levels of RORγt in lung tissues. The AS+DXM group showed a best treatment effect. The results indicated that AS played a therapeutic role for asthma with Yin deficiency syndrome and improved airway inflammation by inhibiting the expression of RORγt in lung tissues and regulating the balance of Th1/Th2 and Th17/Treg.
, 百拇医药
[Key words] Angelicae Sinensis Radix； asthma with Yin deficiency syndrome； Balb/c mouse； Th1/Th2； Th17/Treg
哮喘(asthma)是我國临床中常见的一种呼吸系统疾病，其临床发作具有周期性、反复性、迁延不愈的特点[1-2]。2013年哮喘流行病学调查数据显示，我国大陆地区哮喘总患病率高达1.24%，严重威胁公众健康[3]。研究表明，哮喘发病的主要病理学机制是辅助性T细胞(Th)亚群的免疫功能失调，其中，Th1/Th2与Th17/调节性T细胞(Treg)的功能平衡是影响呼吸道炎性因子分泌及促炎症反应的关键因素[4-5]。近年来，中药以其多途径、多靶点调节机体免疫功能的特点在临床哮喘的治疗中取得优势。当归(Angelica sinensis，AS)为补血养阴活血的常用中药之一，《本草经疏》记载当归有“主咳逆上气”之功效[6]，以往实验研究报道当归挥发油对支气管平滑肌有明显的解痉作用[7]。然而，当归对机体T细胞亚群的免疫调节机制目前尚未完全明确。本研究通过复制阴虚哮喘Balb/c小鼠模型，研究当归对Th1/Th2及Th17/Treg细胞因子的影响，探讨当归治疗哮喘的免疫调节机制，为当归用于临床哮喘的防治提供实验依据。
, 百拇医药
1 材料与方法
1.1 动物、药物与试剂 50只雌性Balb/c小鼠，周龄(7.2±0.8)周，体重(20.15±1.62) g，供应单位为北京中医药大学，合格证0014036，许可证号SCXK(京)2016-0024。当归提取液由上海和黄药业有限公司生产(国药准字Z31020419)。致敏试剂卵清白蛋白(OVA)购自美国Grade V Sigma公司(批号101069301)。甲状腺片购自山东中泰药业有限公司(国药准字H37021712，批号14080303)，地塞米松磷酸钠注射液(DXM)由河南科伦药业有限公司提供(国药准字H41023598，批号13011911)，ELISA细胞因子试剂盒购自R&D systems(美国)，免疫组化试剂盒购自北京博奥森生物技术有限公司(批号AD082522)。, http://www.100md.com(马婷婷 冯兴中 王学艳)