四物汤对幼鼠缺铁性贫血的改善及其对铁代谢的调节作用(1)
[摘要]觀察四物汤对幼鼠缺铁性贫血的改善作用及其对铁代谢调节的机制。该研究用低铁饲料饲喂断乳的SD大鼠2周后，筛选出血红蛋白少于75 g·L-1的大鼠，随机分为模型组，琥珀酸亚铁组(50 mg·kg-1)，四物汤组(4，8，16 g·kg-1)，灌胃给药4周。制作血涂片和肝脏HE染色，测定血常规、血清铁、总铁结合力、血清铁蛋白、肝脏铁、血清铁调素和肝脏铁调素含量，测定肝脏铁蛋白、转铁蛋白和转铁蛋白受体1的表达。结果发现，与正常组相比，模型组活动量减少，耳廓和脚趾呈苍白色；红细胞数量减少，体积变小，中央淡染区扩大；体重和各血常规参数显著降低；肝脏呈浅红色，中央静脉周围的肝细胞索分界不清晰，排列杂乱；血清铁、血清铁蛋白、肝脏铁含量和肝脏铁蛋白表达显著降低；总铁结合力、血清铁调素、肝脏铁调素、转铁蛋白和转铁蛋白受体1表达显著升高，显示造模成功。与模型组相比，四物汤组活动量增多，耳廓和脚趾色泽红润；红细胞数量增多，体积变大，中央淡染区缩小；体重和血常规各参数显著升高；肝脏颜色变红，中央静脉周围的肝细胞索分界较清晰，排列趋于整齐；血清铁、血清铁蛋白、肝脏铁含量和肝脏铁蛋白表达显著升高；总铁结合力、血清铁调素、肝脏铁调素、转铁蛋白和转铁蛋白受体1表达显著降低。研究表明，四物汤对幼鼠缺铁性贫血具有较好的改善作用，其机制可能与改善铁调素对铁代谢的调节有关。
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[关键词] 缺铁性贫血； 四物汤； 幼鼠； 铁代谢； 铁调素
[Abstract] To investigate the effect of Siwu decoction on improving iron deficiency anemia in infant rats and observe its regulatory effects on iron metabolism. SD rats were fed with low iron fodder for 2 weeks， and then the rats with hemoglobin level less than 75 g·L-1 were screened out and randomly divided into model group， Ferrous succinate 50 mg·kg-1 group， Siwu decoction 4 g·kg-1， 8 g·kg-1 and 16 g·kg-1 groups. After 4 weeks′ gavage administration， Wright-Giemsa′s staining of blood smear and HE staining of the livers were conducted， and all rats were tested for blood routine， serum iron， total iron binding capacity， serum ferritin， serum hepcidin and liver hepcidin. The expression levels of liver ferritin， transferrin and transferrin receptor 1 were also detected. The results showed that as compared with normal group， the activity level of model group was decreased， and the color and lustre of auricles and toes were pale white； the number of red blood cells was decreased； the volume was smaller， with an increased zone of central pallor； the body weight and blood routine parameters were decreased significantly； the livers were pale red， and the hepatic cords around thecentral veins were unclear and misaligned； the serum iron， serum ferritin， liver iron levels and the expression of liver ferritin were decreased significantly； the total iron binding capacity， serum hepcidin， liver hepcidin， the expression levels of liver transferrin and transferrin receptor 1 were significantly increased， indicating successful establishment of models.As compared with the model group， activity was increased in Siwu decoction group； the color and lustre of auricles and toes were ruddy； the number of red blood cells was increased； the volume was larger， with a decreased zone of central pallor； the body weight and blood routine parameters were increased significantly； the livers were red， hepatic cords around the central veins were clear and aligned；the serum iron， serum ferritin， liver iron levels and the expression of liver ferritin were significantly increased， the total iron binding capacity， serum hepcidin， liver hepcidin， the expression of liver transferrin and transferrin receptor 1 were decreased significantly. The results demonstrated that Siwu decoction had a certain effect on improving iron deficiency anemia in infant rats， and the mechanism may be associated with the regulatory effect of hepcidin iron metabolism., 百拇医药(何然 汪宏锦 周莹 李晶晶 徐晓玉)
, http://www.100md.com
[关键词] 缺铁性贫血； 四物汤； 幼鼠； 铁代谢； 铁调素
[Abstract] To investigate the effect of Siwu decoction on improving iron deficiency anemia in infant rats and observe its regulatory effects on iron metabolism. SD rats were fed with low iron fodder for 2 weeks， and then the rats with hemoglobin level less than 75 g·L-1 were screened out and randomly divided into model group， Ferrous succinate 50 mg·kg-1 group， Siwu decoction 4 g·kg-1， 8 g·kg-1 and 16 g·kg-1 groups. After 4 weeks′ gavage administration， Wright-Giemsa′s staining of blood smear and HE staining of the livers were conducted， and all rats were tested for blood routine， serum iron， total iron binding capacity， serum ferritin， serum hepcidin and liver hepcidin. The expression levels of liver ferritin， transferrin and transferrin receptor 1 were also detected. The results showed that as compared with normal group， the activity level of model group was decreased， and the color and lustre of auricles and toes were pale white； the number of red blood cells was decreased； the volume was smaller， with an increased zone of central pallor； the body weight and blood routine parameters were decreased significantly； the livers were pale red， and the hepatic cords around thecentral veins were unclear and misaligned； the serum iron， serum ferritin， liver iron levels and the expression of liver ferritin were decreased significantly； the total iron binding capacity， serum hepcidin， liver hepcidin， the expression levels of liver transferrin and transferrin receptor 1 were significantly increased， indicating successful establishment of models.As compared with the model group， activity was increased in Siwu decoction group； the color and lustre of auricles and toes were ruddy； the number of red blood cells was increased； the volume was larger， with a decreased zone of central pallor； the body weight and blood routine parameters were increased significantly； the livers were red， hepatic cords around the central veins were clear and aligned；the serum iron， serum ferritin， liver iron levels and the expression of liver ferritin were significantly increased， the total iron binding capacity， serum hepcidin， liver hepcidin， the expression of liver transferrin and transferrin receptor 1 were decreased significantly. The results demonstrated that Siwu decoction had a certain effect on improving iron deficiency anemia in infant rats， and the mechanism may be associated with the regulatory effect of hepcidin iron metabolism., 百拇医药(何然 汪宏锦 周莹 李晶晶 徐晓玉)