舒郁抑斑方对慢性心理应激促发动脉粥样硬化大鼠的保护作用(1)
[摘要]舒郁抑斑方(SYYB)對伴有慢性心理应激的冠心病患者有较好的治疗作用。为观察SYYB对慢性心理应激促发大鼠动脉粥样硬化(athero-sclerosis，As)早期病变形成及其炎症反应的影响，采用高脂饵食+维生素D3(VD3)腹腔注射建立大鼠As早期病变模型(As)，在此基础上采用不可预知慢性应激刺激法(As+CUMS)，观察慢性心理应激是否加重大鼠病变及SYYB(6.6，13.2，26.4 mg·kg-1)的干预作用。采用悬尾实验和新奇实验法观察大鼠行为学改变，检测血清应激激素促肾上腺素(adrenocortico-tropic hormone，ACTH)、皮质醇(cortisol，Cor)、去甲肾上腺素(noradrenaline，NE)的含量，称量胸腺、肾上腺的质量，综合评价SYYB对模型大鼠心理应激的影响。取主动脉弓HE染色观察血管组织病理改变，测定血清甘油三酯(triglycerides，TG)、总胆固醇(total cholesterol，TC)、高密度脂蛋白(HDL-C)，评价SYYB对大鼠As病变的影响。检测血清高敏C反应蛋白(high-sensitivity C-reactive protein，hsCRP)、白细胞介素-1β(interleukin-1β，IL-1β)、白细胞介素-6(interleukin-6，IL-6)，评价SYYB对As大鼠炎症反应的影响。实验结果显示，与空白组相比，As组TC，TG含量增高，HDL-C明显降低，HE染色可见主动脉壁增厚。As+CUMS组TC，TG含量显著增高，HDL-C明显降低，HE染色可见主动脉弓内膜增厚，泡沫细胞形成，行为学评价及血清应激激素检测结果显示动物存在慢性应激反应，该实验As+CUMS模型复制成功。SYYB可降低血清中TC，TG含量，减轻主动脉泡沫细胞、钙化灶及炎性细胞浸润，对血清中ACTH和Cor，NE有明显的降低作用，同时有效降低了血清中动脉粥样硬化相关的炎性标志物hs-CRP，IL-1和IL-6。提示SYYB对心理应激促发As大鼠的保护作用，其作用与改善神经-内分泌网络平衡及调节相关炎症反应有关。
, http://www.100md.com
[关键词] 舒郁抑斑方； 动脉粥样硬化； 心理应激； 炎症
[Abstract] Shuyu Yiban decoction(SYYB) has significant effect in treating the patients with coronary heart disease combined with chronic psychological stress. In this study， in order to observe the effects of SYYB on early formation of atherosclerosis(As) and inflammation response induced by chronic psychological stress， high-fat diet+intraperitoneal injections of Vitamin D3 were given to establish As early lesion models， and based on these models， chronic unpredictable mild stress(CUMS) was used to observe whether the chronic psychological stress could increase coronary atherosclerotic lesions investigate the intervention effect of SYYB(6.6， 13.2， 26.4 mg·kg-1). The tail suspension test and novelty-feeding test were adopted to detectadrenocortico-tropic hormone(ACTH)， cortisol(Cor) andnoradrenaline(NE) in serum and weigh thymus and adrenal gland， in order to assess the effects of SYYB on CUMS model rats. The pathological changes of vascular tissues in aortic arch were observed by using hematoxylin and eosin(HE) staining， and then the levels of triglycerides(TG)， total cholesterol(TC) and high density lipoprotein(HDL-C) in serum were determined to assess effects of SYYB on As lesions. The effects of SYYB on the inflammatory response in As rats were assessed by detecting high-sensitivity C-reactive protein(hsCRP)， interleukin-1β(IL-1β) and interleukin-6(IL-6) in serum. The results showed that as compared with the blank control group， TC and TG levels in As group were increased while HDL-C was markedly decreased； furthermore， the aortic wall was thickened in HE staining.Meanwhile， foam cells were formed， and the behavioral assessment and serum stress hormone test showed that there was a chronic stress response， indicating successful establishment of As+CUMS models in this study. The experiment demonstrated that SYYB could lower the levels of serum TC and TG， reduce foam cells， calcification and inflammatory cells infiltration. Moreover， SYYB could obviously lower levels of ACTH， Cor and NE and the As related inflammatory indicatorhs-CRP， IL-1β and IL-6 in serum.These results indicated that SYYB had protective effect on chronic psychological stress induced in As rats， and the mechanism was associated with balancing the neuroendocrine-immune network system and regulating inflammation response., http://www.100md.com(郑晓俊 胡志强 栾淑伟 李冉 李赟 李建良 李玉洁 朱晓新)
, http://www.100md.com
[关键词] 舒郁抑斑方； 动脉粥样硬化； 心理应激； 炎症
[Abstract] Shuyu Yiban decoction(SYYB) has significant effect in treating the patients with coronary heart disease combined with chronic psychological stress. In this study， in order to observe the effects of SYYB on early formation of atherosclerosis(As) and inflammation response induced by chronic psychological stress， high-fat diet+intraperitoneal injections of Vitamin D3 were given to establish As early lesion models， and based on these models， chronic unpredictable mild stress(CUMS) was used to observe whether the chronic psychological stress could increase coronary atherosclerotic lesions investigate the intervention effect of SYYB(6.6， 13.2， 26.4 mg·kg-1). The tail suspension test and novelty-feeding test were adopted to detectadrenocortico-tropic hormone(ACTH)， cortisol(Cor) andnoradrenaline(NE) in serum and weigh thymus and adrenal gland， in order to assess the effects of SYYB on CUMS model rats. The pathological changes of vascular tissues in aortic arch were observed by using hematoxylin and eosin(HE) staining， and then the levels of triglycerides(TG)， total cholesterol(TC) and high density lipoprotein(HDL-C) in serum were determined to assess effects of SYYB on As lesions. The effects of SYYB on the inflammatory response in As rats were assessed by detecting high-sensitivity C-reactive protein(hsCRP)， interleukin-1β(IL-1β) and interleukin-6(IL-6) in serum. The results showed that as compared with the blank control group， TC and TG levels in As group were increased while HDL-C was markedly decreased； furthermore， the aortic wall was thickened in HE staining.Meanwhile， foam cells were formed， and the behavioral assessment and serum stress hormone test showed that there was a chronic stress response， indicating successful establishment of As+CUMS models in this study. The experiment demonstrated that SYYB could lower the levels of serum TC and TG， reduce foam cells， calcification and inflammatory cells infiltration. Moreover， SYYB could obviously lower levels of ACTH， Cor and NE and the As related inflammatory indicatorhs-CRP， IL-1β and IL-6 in serum.These results indicated that SYYB had protective effect on chronic psychological stress induced in As rats， and the mechanism was associated with balancing the neuroendocrine-immune network system and regulating inflammation response., http://www.100md.com(郑晓俊 胡志强 栾淑伟 李冉 李赟 李建良 李玉洁 朱晓新)