延龄草与吉林延龄草根茎及须根中3种皂苷成分的含量测定(1)
[摘要]比较延龄草与吉林延龄草药材根茎与须根间主要成分的含量差异，利用HPLCCAD同时测定了3种主要成分重楼皂苷Ⅶ、偏诺皂苷元3βOαL吡喃鼠李糖基(1→4)[αL吡喃鼠李糖基(1→2)]OβD葡萄糖、重楼皂苷Ⅵ的含量。色谱条件：TSK gel ODS(46 mm×250 mm， 5 μm)色谱柱，乙腈水(43∶57)等度洗脱，流速为1 mL·min-1，进样量为20 μL。电雾式检测器(CAD)：工作气压为35 psi(1 psi=6895 kPa)，雾化器温度为35 ℃。3种甾体皂苷良好分离，线性关系、稳定性、精密度、重复性、加样回收试验均符合中药质量分析要求。运用IBM SPSS Statistics 20分析软件进行主成分分析，结果显示，头顶一颗珠的来源包含了2种延龄草属植物，且吉林延龄草为主要资源；7批延龄草样品和9批吉林延龄草的根茎之间3种皂苷成分的含量存在不显著差异；但是根茎与须根之间3种皂苷成分的含量存在显著的差异。
[关键词]HPLCCAD； 延龄草与吉林延龄草； 根茎与须根； 甾体皂苷； 含量测定； 主成分分析
, 百拇医药
[Abstract]To compare the differences of main components between in rhizoma and fibrous root of Trillium tschonoskii and T kamtschaticum， a simple， accurate and reliable high performance liquid chromatography coupled with the charged aerosol detector (HPLCCAD) method was developed and then successfully applied for simultaneous quantitative analysis of three compounds， including polyphyllin Ⅶ (T1)，pennogenin 3OαLrhamnopyranosyl(1→2) [αLrhamnopyranosyl(1→4)]βDglucopyranoside (T2)，polyphyllin Ⅵ (T3)， in 16 batches of rhizome and 14 batches of fibrous root The analytes were well separated from other constituents on TSK gel ODS (46 mm×250 mm， 5 μm) with acetonitrilewater (43∶57) at a flow rate of 10 mL·min-1 The injection volume was 20 μL The nitrogen inlet pressure for the CAD system was 35 psi and the nebulizer chamber temperature was 35 ℃The method was validated for linearity (r>0999 0)， intra and interday precision (029%30%)， repeatability (045%14%)， stability (19%26%)， recovery (1001%1002%， 12%18%)， limits of detection (0002 g·L-1)， and limits of quantification (0005 g·L-1)The obtained datasets were processed by principal component analysis (PCA) and it showed that there was almost no difference in rhizoma of T tschonoskii and T kamtschaticum from different areas of China However， the 3 major compounds existed in rhizoma were different from those in fibrous root of T tschonoskii and T kamtschaticum
, 百拇医药
[Key words]HPLCCAD； Trillium tschonoskii and Trillium kamtschaticum； rhizoma and fibrous root； steroid saponin； determination； principal component analysis (PCA)
延龄草为百合科延龄草属植物，全世界共有约50种，我国有3种，即延龄草Trillium tschonoskii Maxim、吉林延龄草T kamtschaticum Pall，分布于吉林、西藏延龄草T govanianum Wall(仅见于西藏卡马河下游)[1]。其中，延龄草T tschonoskii被《湖北省中药材标准》收录，药材名头顶一颗珠，又名芋儿七、佛手七、狮儿七等，药用部位为根及根茎。其味甘、性平、有小毒，有延年益寿的功效，主治头晕目眩、跌打损伤、神经衰弱、高血压病和脑震荡后遗症等疾病，为土家族常用药材[23]。本课题组在进行头顶一颗珠的产地和市场资源调查时发现，目前河北安国、安徽亳州、四川成都和广西玉林等药材市场流通的头顶一颗珠药材均为来自吉林的吉林延龄草，安徽亳州市场的药材也以黑龙江产為主，中药材天地网诚实通平台销售的头顶一颗珠也是吉林延龄草，表明头顶一颗珠药材的来源值得研究。
延龄草中主要的化学成分为甾体皂苷，黄酮苷、倍半萜苷、苯丙素苷等[4]，目前已有延龄草药材中总皂苷及主要甾体皂苷成分含量测定的研究报道[56]，但迄今为止尚未有吉林延龄草的质量研究和相关报道。本研究利用HPLCCAD测定2种延龄草药用部位(根状茎和须根)中重楼皂苷Ⅶ、偏诺皂苷元3βOαL吡喃鼠李糖基(1→4)[αL吡喃鼠李糖基(1→2)]OβD葡萄糖、重楼皂苷Ⅵ等3种主要皂苷的含量，其中重楼皂苷Ⅶ和重楼皂苷Ⅵ也是重楼中的重要活性成分[78]。同时，利用统计学方法分析几个重要产区的延龄草与吉林延龄草之间及根茎与须根之间化学成分的差异，为延龄草属药材合理利用及质量评价提供了科学依据。, http://www.100md.com(杨印军 孙欣光 杨杰 李齐 张洁 赵阳 马百平 郭宝林)
[关键词]HPLCCAD； 延龄草与吉林延龄草； 根茎与须根； 甾体皂苷； 含量测定； 主成分分析
, 百拇医药
[Abstract]To compare the differences of main components between in rhizoma and fibrous root of Trillium tschonoskii and T kamtschaticum， a simple， accurate and reliable high performance liquid chromatography coupled with the charged aerosol detector (HPLCCAD) method was developed and then successfully applied for simultaneous quantitative analysis of three compounds， including polyphyllin Ⅶ (T1)，pennogenin 3OαLrhamnopyranosyl(1→2) [αLrhamnopyranosyl(1→4)]βDglucopyranoside (T2)，polyphyllin Ⅵ (T3)， in 16 batches of rhizome and 14 batches of fibrous root The analytes were well separated from other constituents on TSK gel ODS (46 mm×250 mm， 5 μm) with acetonitrilewater (43∶57) at a flow rate of 10 mL·min-1 The injection volume was 20 μL The nitrogen inlet pressure for the CAD system was 35 psi and the nebulizer chamber temperature was 35 ℃The method was validated for linearity (r>0999 0)， intra and interday precision (029%30%)， repeatability (045%14%)， stability (19%26%)， recovery (1001%1002%， 12%18%)， limits of detection (0002 g·L-1)， and limits of quantification (0005 g·L-1)The obtained datasets were processed by principal component analysis (PCA) and it showed that there was almost no difference in rhizoma of T tschonoskii and T kamtschaticum from different areas of China However， the 3 major compounds existed in rhizoma were different from those in fibrous root of T tschonoskii and T kamtschaticum
, 百拇医药
[Key words]HPLCCAD； Trillium tschonoskii and Trillium kamtschaticum； rhizoma and fibrous root； steroid saponin； determination； principal component analysis (PCA)
延龄草为百合科延龄草属植物，全世界共有约50种，我国有3种，即延龄草Trillium tschonoskii Maxim、吉林延龄草T kamtschaticum Pall，分布于吉林、西藏延龄草T govanianum Wall(仅见于西藏卡马河下游)[1]。其中，延龄草T tschonoskii被《湖北省中药材标准》收录，药材名头顶一颗珠，又名芋儿七、佛手七、狮儿七等，药用部位为根及根茎。其味甘、性平、有小毒，有延年益寿的功效，主治头晕目眩、跌打损伤、神经衰弱、高血压病和脑震荡后遗症等疾病，为土家族常用药材[23]。本课题组在进行头顶一颗珠的产地和市场资源调查时发现，目前河北安国、安徽亳州、四川成都和广西玉林等药材市场流通的头顶一颗珠药材均为来自吉林的吉林延龄草，安徽亳州市场的药材也以黑龙江产為主，中药材天地网诚实通平台销售的头顶一颗珠也是吉林延龄草，表明头顶一颗珠药材的来源值得研究。
延龄草中主要的化学成分为甾体皂苷，黄酮苷、倍半萜苷、苯丙素苷等[4]，目前已有延龄草药材中总皂苷及主要甾体皂苷成分含量测定的研究报道[56]，但迄今为止尚未有吉林延龄草的质量研究和相关报道。本研究利用HPLCCAD测定2种延龄草药用部位(根状茎和须根)中重楼皂苷Ⅶ、偏诺皂苷元3βOαL吡喃鼠李糖基(1→4)[αL吡喃鼠李糖基(1→2)]OβD葡萄糖、重楼皂苷Ⅵ等3种主要皂苷的含量，其中重楼皂苷Ⅶ和重楼皂苷Ⅵ也是重楼中的重要活性成分[78]。同时，利用统计学方法分析几个重要产区的延龄草与吉林延龄草之间及根茎与须根之间化学成分的差异，为延龄草属药材合理利用及质量评价提供了科学依据。, http://www.100md.com(杨印军 孙欣光 杨杰 李齐 张洁 赵阳 马百平 郭宝林)