淹水胁迫对杭菊花色苷及合成相关酶和基因的影响(1)
[摘要] 测定不同开放程度杭菊头状花序花色苷含量及其相关基因表达量和相关酶活性和含量,分析淹水胁迫对杭菊头状花序开放过程中花色苷合成的影响。结果发现杭菊CHS基因表达量和CHS酶含量随杭菊头状花序开放程度的增加呈現先上升后下降的趋势,而花色苷含量、DFR基因表达量和DFR酶活性均随杭菊头状花序开放程度的增加而呈现先下降后上升的趋势,花色苷与DFR基因和DFR酶呈显著正相关,而与CHS基因和CHS酶无显著相关性。说明淹水胁迫对杭菊花色苷的合成及其相关酶和基因有显著影响,但不改变它们在杭菊头状花序开放过程中的变化规律,DFR基因和DFR酶的变化规律与花色苷一致,确实为花色苷合成的关键基因和关键酶,而CHS基因和CHS酶含量与花色苷的含量无显著相关性,CHS基因和CHS酶是否为杭菊花色苷合成关键基因和关键酶还有待进一步研究。
[关键词] 杭菊; 二氢黄酮醇还原酶; 查尔酮合成酶; 花色苷
[Abstract] The study is aimed at determine the content of anthocyanins and expressions of relative genes and activity of relative enzymes. The effects of flood stress on anthocyanins synthesis with relative genes and enzymes of Chrysanthemum morifolium cv. ′Hangju′ were analyzed. The expression of CHS and the content of CHS presented the trend of first rising and after downward with the increase of flowering degree. The content of anthocyanins、the expression of DFR and the activity of DFR presented the trend of first downward and after rising with the increase of flowering degree. There was a positive correlation among anthocyanins ......
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[关键词] 杭菊; 二氢黄酮醇还原酶; 查尔酮合成酶; 花色苷
[Abstract] The study is aimed at determine the content of anthocyanins and expressions of relative genes and activity of relative enzymes. The effects of flood stress on anthocyanins synthesis with relative genes and enzymes of Chrysanthemum morifolium cv. ′Hangju′ were analyzed. The expression of CHS and the content of CHS presented the trend of first rising and after downward with the increase of flowering degree. The content of anthocyanins、the expression of DFR and the activity of DFR presented the trend of first downward and after rising with the increase of flowering degree. There was a positive correlation among anthocyanins ......
您现在查看是摘要页,全文长 3566 字符。