桑椹多糖的结构表征及其对乙醇脱氢酶活性的影响研究(1)
[摘要] 该实验采用回流法提取桑椹多糖，经脱蛋白及DEAE-Cellulose 52分离纯化得到4种多糖组分(中性多糖MFP-1，微酸性多糖MFP-2，酸性多糖MFP-3和MFP-4)。通过化学法和仪器分析法对各多糖组分进行理化性质测定和结构表征，并研究其对乙醇脱氢酶(ADH)活性的影响。结果表明，MFP-1，MFP-2，MFP-3和MFP-4中的多糖量分别为75.3%，83.7%，79.1%，74.3%，其平均相对分子质量分别为112.2，128.8，199.5，181.9 kDa。MFP-1主要含有半乳糖、葡萄糖、阿拉伯糖、鼠李糖、甘露糖(26.8∶20.4∶8.7∶5∶1)；MFP-2主要含有阿拉伯糖、半乳糖、鼠李糖、葡萄糖、半乳糖醛酸(34.2∶38.2∶8∶17.5∶15.1)；MFP-3主要含有半乳糖醛酸、半乳糖、葡萄糖、鼠李糖、阿拉伯糖(28.3∶22.6∶20.9∶18.6∶12.5)；MFP-4主要含有葡萄糖、半乳糖、鼠李糖、半乳糖醛酸、阿拉伯糖、葡萄糖醛酸(47.4∶34.9∶36.1∶33.1∶19.9∶4.1)。红外光谱分析结果表明，MFP-1，MFP-2，MFP-3和MFP-4中均有多糖的特征吸收峰，其中MFP-3和MFP-4是以β苷键为主的吡喃型多糖。活性结果表明，4种多糖对ADH均有激活作用，其ADH激活能力顺序为：联苯双酯>MFP-3>MFP-1>MFP-2>硫普罗宁>MFP-4，MFP-1，MFP-2和MFP-3对ADH的激活作用均大于阳性对照硫普罗宁，同时发现MFP-3对ADH的激活效果与阳性对照联苯双酯的激活效果相当，推测其与之连接的糖醛酸种类有关，这为桑椹多糖的“构-效”研究提供参考依据。
[关键词] 桑椹多糖； 理化性质； 结构表征； ADH活性
[Abstract] Mori Fructus polysaccharides (MFPs) have been used as a source of therapeutic agents. The most promising activities of these biopolymers are their immunomodulation and anti-cancer effects. It was reported that polysaccharides were a potential drug against liver injury， but the hepatoprotective effect of MFPs was ambiguous. In this study， the fractionation of crude polysaccharides on DEAE-Cellulose 52 gave four fractions (MFP-1， MFP-2， MFP-2 and MFP-4). The results showed that the contents of carbohydrates were 75.3%， 83.7%， 79.1%， 74.3%， and the molecular weight of them were 112.2， 128.8， 199.5， 181.9 kDa. Monosaccharide component analysis indicated that MFP-1 was composed of galactose， glucose， arabinose， rhamnose and mannose with the molarity rate of 26.8∶20.4∶8.74∶5∶1； MFP-2 contained arabinose， galactose， rhamnose， glucose and galacturonic acid with the molarity rate of 34.2∶38.2∶8∶17.5∶15.1； MFP-3 was composed of galacturonic acid， galactose， glucose， rhamnose and arabinose with the molarity rate of 28.3∶22.6∶20.9∶18.6∶15.1； MFP-4 contained glucose， galactose， rhamnose， galacturonic acid， arabinose and glucuronic acid with the molarity rate of 47.4∶34.9∶36.1∶33.1∶19.9∶4.1. IR analysis′s results indicated that MFP-3 and MFP-4 may be polysaccharides containing β-glycosides. The alcohol dehydrogenase (ADH) activity text showed that， all these four MFPs were found have a positive effect on the activity of ADH， with order： bifendate>MFP-3>MFP-1>MFP-2>tioprnin>MFP-4， and the MFP-3 had the highest activity and demonstrated outstanding hepatic protecting activity.
[Key words] Mori Fructus polysaccharides； physic-chemical characters； characterization； activity effect of ADH
據2015年版药典记载[1]，桑椹具有滋阴补血、生津润燥的药效，用于肝肾阴虚、眩晕耳鸣、心悸失眠、须发早白、津伤口渴、内热消渴、肠燥便秘等。此外，还有少量文献报道[2]桑椹对酒精/四氯化碳所致的肝损伤有潜在的保护作用，国家已把桑椹列为药食同源的保健品行列。 (王杏 邓青芳 陈华国 周欣)
[关键词] 桑椹多糖； 理化性质； 结构表征； ADH活性
[Abstract] Mori Fructus polysaccharides (MFPs) have been used as a source of therapeutic agents. The most promising activities of these biopolymers are their immunomodulation and anti-cancer effects. It was reported that polysaccharides were a potential drug against liver injury， but the hepatoprotective effect of MFPs was ambiguous. In this study， the fractionation of crude polysaccharides on DEAE-Cellulose 52 gave four fractions (MFP-1， MFP-2， MFP-2 and MFP-4). The results showed that the contents of carbohydrates were 75.3%， 83.7%， 79.1%， 74.3%， and the molecular weight of them were 112.2， 128.8， 199.5， 181.9 kDa. Monosaccharide component analysis indicated that MFP-1 was composed of galactose， glucose， arabinose， rhamnose and mannose with the molarity rate of 26.8∶20.4∶8.74∶5∶1； MFP-2 contained arabinose， galactose， rhamnose， glucose and galacturonic acid with the molarity rate of 34.2∶38.2∶8∶17.5∶15.1； MFP-3 was composed of galacturonic acid， galactose， glucose， rhamnose and arabinose with the molarity rate of 28.3∶22.6∶20.9∶18.6∶15.1； MFP-4 contained glucose， galactose， rhamnose， galacturonic acid， arabinose and glucuronic acid with the molarity rate of 47.4∶34.9∶36.1∶33.1∶19.9∶4.1. IR analysis′s results indicated that MFP-3 and MFP-4 may be polysaccharides containing β-glycosides. The alcohol dehydrogenase (ADH) activity text showed that， all these four MFPs were found have a positive effect on the activity of ADH， with order： bifendate>MFP-3>MFP-1>MFP-2>tioprnin>MFP-4， and the MFP-3 had the highest activity and demonstrated outstanding hepatic protecting activity.
[Key words] Mori Fructus polysaccharides； physic-chemical characters； characterization； activity effect of ADH
據2015年版药典记载[1]，桑椹具有滋阴补血、生津润燥的药效，用于肝肾阴虚、眩晕耳鸣、心悸失眠、须发早白、津伤口渴、内热消渴、肠燥便秘等。此外，还有少量文献报道[2]桑椹对酒精/四氯化碳所致的肝损伤有潜在的保护作用，国家已把桑椹列为药食同源的保健品行列。 (王杏 邓青芳 陈华国 周欣)