基于LCQTOFMS/MS技术分析不同产地杜仲的差异化学成分(1)
[摘要]为了探讨生态环境对杜仲代谢物合成积累的影响，本文采用液相色谱串联三重四级杆飞行时间高分辨质谱(LCQTOF MS/MS)结合多元统计分析技术对不同产地杜仲中化学成分差异性进行研究。通过二级串联质谱分析，对其质谱数据进行峰匹配、峰对齐、滤噪处理等，并进行特征峰提取；用主成分分析(PCA)和偏最小二乘法判别分析(PLSDA)进行数据处理；根据一级质谱精确质荷比和二级质谱碎片信息，结合软件数据库搜索、标准品比对及相关文献进行成分鉴定。结果显示，3个不同产地杜仲样品间的化学组成得到有效区分；初步鉴定出23个差异化学成分，其中14种共有差异化学成分呈现不同的变化规律。该结果为揭示生态环境对杜仲代谢物合成积累的影响规律提供基础资料。
[关键词]杜仲； 液相色谱串联三重四极杆飞行时间高分辨质谱； 产地； 化学成分
[Abstract]In order to study the influence of ecological environment regarding the synthesis and accumulation of metabolites in Eucommiae Cortex， LCQTOF MS/MS method combined with multivariate statistical analysis was used to analyze the differences of chemical constituents in Eucommiae Cortex from different habitats Through the analysis of the multistage tandem mass spectrometry， the characteristic peaks were extracted with mass spectrometry data peak matching， peak alignment， and noise filtering Principal component analysis (PCA) and partial leastsquares discriminant analysis (PLSDA) were used for data processing The chemical constituents were identified or tentative presumed according to MS accurate mass and MS/MS spectrometry fragmentation information， combined with the software of database search， comparison with reference standards and literature The results show the differences among samples of Eucommiae Cortex from different habitats are distinguishable A total of 23 chemical constituents in Eucommiae Cortex were identified or tentative presumed Among of them， 14 kinds of common differential chemical constituents (aucubin， geniposidic acid， neochlorogenic acid， syringin， olivil4′，4′diOβDglucopyranoside， chlorogenic acid， cryptochlorogenic acid， 1hydroxypinoresinol 4′，4′diOβDglucopyranoside， caffeic acid， pinoresinoldiOβDglucopyranoside， syringaresionldiOβDglucopyranoside， pinoresinol4′OβDglucopyranoside， eucommiol， isochlorogenic acid C and asiatic acid) presented different changing laws This study provides basic information for revealing the influence law of ecological environment on the biosynthesis of metabolites in Eucommiae Cortex
, http://www.100md.com
[Key words]Eucommiae Cortex； LCQTOF MS/MS； habitats； chemical constituents
杜仲為我国特有的名贵药材，药用历史悠久，始载于《神农本草经》，列为上品，来源于杜仲科植物杜仲Eucommia ulmoides Oilv的干燥树皮，具有补肝肾、强筋骨、安胎之功效，用于肝肾不足，腰膝酸痛，筋骨无力，头晕目眩，妊娠漏血，胎动不安等症[1]。现代研究表明，杜仲含有木脂素类、环烯醚萜类、苯丙素类、多糖等多类药效活性成分，具有降血压、降血脂、降血糖、抗肿瘤、抗菌抗病毒、保肝护肾、抗骨质疏松等多方面药理作用[23]。杜仲属落叶乔木，在我国主要分布在秦岭以南华中地区的四川、贵州、湖南、湖北、陕西等省。近年来我国杜仲引种栽培，资源不断扩大，市售杜仲药材主要为栽培品。由于杜仲植物地理分布广，形体变化大，药材品质差异大，药材质量参差不齐，难以实现商品药材标准化[4]。目前对杜仲药材的研究主要集中于化学成分、药理作用和品质评价方面，对于不同产地杜仲药材的研究主要集中于主要成分含量变化，亦有指纹图谱研究，紫外谱线组鉴别等[57]，运用植物代谢组学技术分析不同产地杜仲中化学成分差异的研究还很少。, 百拇医药(严颖 赵慧 邹立思 刘训红 柴川 王胜男 华愉教)
[关键词]杜仲； 液相色谱串联三重四极杆飞行时间高分辨质谱； 产地； 化学成分
[Abstract]In order to study the influence of ecological environment regarding the synthesis and accumulation of metabolites in Eucommiae Cortex， LCQTOF MS/MS method combined with multivariate statistical analysis was used to analyze the differences of chemical constituents in Eucommiae Cortex from different habitats Through the analysis of the multistage tandem mass spectrometry， the characteristic peaks were extracted with mass spectrometry data peak matching， peak alignment， and noise filtering Principal component analysis (PCA) and partial leastsquares discriminant analysis (PLSDA) were used for data processing The chemical constituents were identified or tentative presumed according to MS accurate mass and MS/MS spectrometry fragmentation information， combined with the software of database search， comparison with reference standards and literature The results show the differences among samples of Eucommiae Cortex from different habitats are distinguishable A total of 23 chemical constituents in Eucommiae Cortex were identified or tentative presumed Among of them， 14 kinds of common differential chemical constituents (aucubin， geniposidic acid， neochlorogenic acid， syringin， olivil4′，4′diOβDglucopyranoside， chlorogenic acid， cryptochlorogenic acid， 1hydroxypinoresinol 4′，4′diOβDglucopyranoside， caffeic acid， pinoresinoldiOβDglucopyranoside， syringaresionldiOβDglucopyranoside， pinoresinol4′OβDglucopyranoside， eucommiol， isochlorogenic acid C and asiatic acid) presented different changing laws This study provides basic information for revealing the influence law of ecological environment on the biosynthesis of metabolites in Eucommiae Cortex
, http://www.100md.com
[Key words]Eucommiae Cortex； LCQTOF MS/MS； habitats； chemical constituents
杜仲為我国特有的名贵药材，药用历史悠久，始载于《神农本草经》，列为上品，来源于杜仲科植物杜仲Eucommia ulmoides Oilv的干燥树皮，具有补肝肾、强筋骨、安胎之功效，用于肝肾不足，腰膝酸痛，筋骨无力，头晕目眩，妊娠漏血，胎动不安等症[1]。现代研究表明，杜仲含有木脂素类、环烯醚萜类、苯丙素类、多糖等多类药效活性成分，具有降血压、降血脂、降血糖、抗肿瘤、抗菌抗病毒、保肝护肾、抗骨质疏松等多方面药理作用[23]。杜仲属落叶乔木，在我国主要分布在秦岭以南华中地区的四川、贵州、湖南、湖北、陕西等省。近年来我国杜仲引种栽培，资源不断扩大，市售杜仲药材主要为栽培品。由于杜仲植物地理分布广，形体变化大，药材品质差异大，药材质量参差不齐，难以实现商品药材标准化[4]。目前对杜仲药材的研究主要集中于化学成分、药理作用和品质评价方面，对于不同产地杜仲药材的研究主要集中于主要成分含量变化，亦有指纹图谱研究，紫外谱线组鉴别等[57]，运用植物代谢组学技术分析不同产地杜仲中化学成分差异的研究还很少。, 百拇医药(严颖 赵慧 邹立思 刘训红 柴川 王胜男 华愉教)