基于DNA条形码技术的维吾尔药材桑葚基原鉴定研究(1)
[摘要]該研究通过分析多途径来源的新疆桑属植物及药材样本的ITS2，psbAtrnH序列，为药材分子鉴定提供依据。以51个新疆桑属植物及药材为样本，对其ITS2，psbAtrnH序列进行PCR扩增和测序，用MEGA 60计算其种内、种间Kimura 2parameter(K2P)距离，分析变异位点，并构建NJ鉴别树。ITS2序列分析结果显示，桑Morus alba、鞑靼桑M alba var tatarica、黑桑M nigra种内无变异；桑与鞑靼桑种间无变异；桑与黑桑种间存在13个变异位点，种间平均KP2遗传距离为004；桑与药材样本间无信息变异位点，NJ鉴别树可将桑及鞑靼桑与黑桑区分。psbAtrnH序列分析结果显示，桑与黑桑种内各有1个变异位点，3种植物种间存在插入/缺失变异，可相互区分；种间变异与药材样本内变异一致。因此，ITS2序列可将来源于桑、鞑靼桑的药材样本与黑桑区分，psbAtrnH序列可将来源于三者的药材样本区分，为维吾尔药材真伪鉴别及市场监管提供依据。
[关键词]维吾尔药； 桑； DNA条形码； ITS2； psbAtrnH
, http://www.100md.com
Identification of origin plant of Uygur medicine mulberry
based on DNA barcode
FAN Congzhao1， XU Jianguo1， LI Yawei2， LI Xiaojin1*
(1Xinjiang Institute of Chinese Materia Medica and Ethnical Materia State Administration of Traditional Chinese
Medicine， Key Laboratory of Traditional Chinese Medicine and Ethnic Medicine Resources， Urumqi 830002， China；
, http://www.100md.com
2 Testing Center of Xinjiang Entry Exit Inspection and Quarantine Portal， Urumqi 830063， China)
[Abstract]To provide molecular evidence for medical material identification， we analyzed the nucleotide sequence of ITS2， psbAtrnH gene in Morus genus plants and commercial products which were obtained from different places in Xinjiang The sequence of ITS2 and psbAtrnH in fiftyone samples were amplified and sequenced， MEGA 60 was used to analyze the intra and interspecific K2P distances， neighborjoining (NJ) tree was used to constructing clustering tree ITS2 sequence analyzed results showed that there is no intraspecific variation among Morus alba， M alba var tatarica and M nigra， but 13 variations sites were exist between M alba and M nigra and their interspecific K2P distances was 004， which indicated that there had significant variation in them We didn′t find informative variation sites between Morus genus plants and commercial products， and we also found that M nigra can be distinguished from other two species by NJ Tree PsbAtrnH analysis results showed there was only one variation site between M alba and M nigra， but insertion or deletion variation were remarkable evidence among M alba， M alba var tatarica and M Nigra Interspecific variation was accordance with intraspecific variation of commercial products So ITS2 and psbAtrnH gene were important marker for M alba， M alba var tatarica and M nigra identification This study provided important evidence for Uygur medicine identification and market supervision
[Key words]Uygur medicine； mulberry； DNA barcode； ITS2； psbAtrnH, http://www.100md.com(樊丛照徐建国李亚伟李晓瑾)
[关键词]维吾尔药； 桑； DNA条形码； ITS2； psbAtrnH
, http://www.100md.com
Identification of origin plant of Uygur medicine mulberry
based on DNA barcode
FAN Congzhao1， XU Jianguo1， LI Yawei2， LI Xiaojin1*
(1Xinjiang Institute of Chinese Materia Medica and Ethnical Materia State Administration of Traditional Chinese
Medicine， Key Laboratory of Traditional Chinese Medicine and Ethnic Medicine Resources， Urumqi 830002， China；
, http://www.100md.com
2 Testing Center of Xinjiang Entry Exit Inspection and Quarantine Portal， Urumqi 830063， China)
[Abstract]To provide molecular evidence for medical material identification， we analyzed the nucleotide sequence of ITS2， psbAtrnH gene in Morus genus plants and commercial products which were obtained from different places in Xinjiang The sequence of ITS2 and psbAtrnH in fiftyone samples were amplified and sequenced， MEGA 60 was used to analyze the intra and interspecific K2P distances， neighborjoining (NJ) tree was used to constructing clustering tree ITS2 sequence analyzed results showed that there is no intraspecific variation among Morus alba， M alba var tatarica and M nigra， but 13 variations sites were exist between M alba and M nigra and their interspecific K2P distances was 004， which indicated that there had significant variation in them We didn′t find informative variation sites between Morus genus plants and commercial products， and we also found that M nigra can be distinguished from other two species by NJ Tree PsbAtrnH analysis results showed there was only one variation site between M alba and M nigra， but insertion or deletion variation were remarkable evidence among M alba， M alba var tatarica and M Nigra Interspecific variation was accordance with intraspecific variation of commercial products So ITS2 and psbAtrnH gene were important marker for M alba， M alba var tatarica and M nigra identification This study provided important evidence for Uygur medicine identification and market supervision
[Key words]Uygur medicine； mulberry； DNA barcode； ITS2； psbAtrnH, http://www.100md.com(樊丛照徐建国李亚伟李晓瑾)